HOME JOURNALS CONTACT

Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 6 | Page No.: 591-595
DOI: 10.3923/jbs.2003.591.595
Effect of Nitrogen and Phosphate Sources on the Biosynthesis of β-Fructofuranosidase
Mirza Ahsen Baig, Kiran Shafiq , Shazia Mirza , Sikander Ali and Ikram-ul-Haq

Abstract: Saccharomyces species GCA-II was used to investigate the effect of mineral constituents on the production of β -fructofuranosidase in submerged fermentation. Different organic nitrogen and phosphate sources in varied concentrations were tested for optimal production of enzyme. The results indicated that enzyme production increased from 107.42 to 168.58 U ml-1. Thus, Saccharomyces strain GCA-II gave maximal β -fructofuranosidase in submerged fermentation when urea (3.0 g l-1) as a nitrogen source and K2HPO4 (0.20 g l-1) as phosphate source was supplied in fermentation medium.

Fulltext PDF

How to cite this article
Mirza Ahsen Baig, Kiran Shafiq , Shazia Mirza , Sikander Ali and Ikram-ul-Haq , 2003. Effect of Nitrogen and Phosphate Sources on the Biosynthesis of β-Fructofuranosidase. Journal of Biological Sciences, 3: 591-595.

Keywords: phosphjate, oranic nitrogen, saccharomyces, mineral constituesnts, b- fructofuranosidase and sunmerged fermentation

REFERENCES
  • Ashokkumar, B., N. Kyalvizhi and P. Gunasekaran, 2001. Optimization of media for β-fructofuranosidase production by Aspergillus niger in submerged and solid state fermentation. Process Biochem., 37: 331-338.
    CrossRef    Direct Link    

  • Dworschack, R.G. and L.J. Wickerham, 1960. Extracellular invertase by sucrose-fermenting yeasts. U.S. Patent No. 2953500.

  • Egorov, S.N., I.N. Semenova and V.N. Maksimov, 2000. Mutual effect of invertase and acid phosphatase from the yeast Saccharomyces cerevisiae on their secretion into culture media. Mikrobiologiia, 69: 34-37.

  • Gomez, S.J.R., C. Augur and G. Viniegra-Gozalez, 2000. Invertase production by Aspergillus niger in submerged and solid-state fermentation. Biotechnol. Lett., 22: 1255-1258.
    CrossRef    Direct Link    

  • Haq, I., K. Shafiq, A. Sikander and M.A. Qadeer, 2002. Production of enzyme invertase by Saccharomyces cerevisiae. Indian J. Plant Sci., 1: 5-8.

  • Pitombo, R.N.M., C. Spring, R.F. Passos, M. Tonato and M. Vitole, 1994. Effect of moisture content on invertase activity of freeze-dried Saccharomyces cerevisiae. Cytobiology, 31: 383-392.
    PubMed    Direct Link    

  • Kiran, S., S. Ali and Ikram-ul-Haq, 2002. Effect of different mineral nutrients on invertase production by Saccharomyces cerevisiae GCB-K5. Biotechnology, 1: 40-44.
    CrossRef    Direct Link    

  • Silveira, M.C., E.M. Oliveira, E. Carvajal and E.P. Bon, 2000. Nitrogen regulation of Saccharomyces cerevisiae invertase. Role of the URE2 gene. Applied Biochem. Biotechnol., 84-86: 247-254.
    Direct Link    

  • Sumner, J.B. and S.F. Howell, 1935. A method for determination of saccharase activity. J. Biol. Chem., 108: 51-54.
    Direct Link    

  • Tasun, K., P. Chose and K. Glien, 1970. Sugar determination of DNS method. Biotechnol. Bioeng., 12: 921-921.

    • © Science Alert. All Rights Reserved