HOME JOURNALS CONTACT

Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 2 | Page No.: 167-171
DOI: 10.3923/jbs.2003.167.171
In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties
M. Asaduzzaman, M. A. Bari, M. H. Rahman, N. Khatun , M.A. Islam and M. Rahman

Abstract: Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.

Fulltext PDF

How to cite this article
M. Asaduzzaman, M. A. Bari, M. H. Rahman, N. Khatun , M.A. Islam and M. Rahman , 2003. In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties. Journal of Biological Sciences, 3: 167-171.

Keywords: Rice, anther culture, rgeneration and in vitro

REFERENCES
  • Cao, M.Q., F. Li, T. Jiang and G.S. Li, 1995. Application of anther culture and isolated micro spore culture to vegetable crops improvement. Acta Hortic., 392: 27-38.

  • Chen, C. and M. Lin, 1976. Induction of rice plantlets from anther culture. Bot. Bull. Acad. Sin., 17: 18-24.

  • Chu, C.C., 1982. Haploid in Plant Improvement. In: Plant Improvement and Somatic Cell Genetics, Vasil, I.K., W.K. Scowcroft and K.J. Frey (Eds.). Academic Press, New York, pp: 129-158

  • Chu, C.C., C.C. Wang, C.S. Sun, K.C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi, 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scient. Sin., 18: 659-668.
    Direct Link    

  • Datta, S.K., K. Datta and I. Potrykus, 1990. Embryogenesis and plant regeneration from micro spores of both Indical and Japonica rice (Oryza sativa). Plant Sci., 7: 83-88.

  • Dunwell, J.M., 1986. Pollen, Ovule and Embryo Culture as Tools in Plant Breeding. In: Plant Tissue Culture and its Agricultural Application, Withers, L.A. and P.G. Alderson (Eds.). Butterworths, London, pp: 375-404

  • Guha-Mukejee, S., 1973. Genotypic differences in vitro formation and embryoids from rice pollen. J. Exp. Bot., 24: 139-144.

  • Iyer, R.D. and S.K. Raina, 1972. The early ontogeny of embryoids and callus from pollen and subsequent organogenesis in anther cultures of Datura metel and rice. Planta, 104: 146-156.

  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
    CrossRef    Direct Link    

  • Niizeki, H. and K. Oono, 1968. Induction of haploid rice plants from another culture. Proc. Jap. Acad., 44: 554-557.

  • Villenx, R.E., 1994. Development of new cultivars via anther culture. Hortic. Sci., 29: 1238-1240.
    Direct Link    

  • Chen, C.C., 1977. In vitro development of plants from micro spores of rice. In vitro, 13: 484-489.
    Direct Link    

  • Karim, N.H.N., A.K.M. Shahjahan, Maksuda, A. Nahar, S.A. Miah and M.Z. Haque, 1991. Improved media for callus induction from anthers of indica rice (Oryza sativa). Plant Tissue Cult., 1: 43-50.

  • Oono, K., 1975. Production of haploid plants of rice by anther culture and their use in breeding. Bull. Nat. Inst. Agric. Sci., 260: 139-222.

    • © Science Alert. All Rights Reserved