Abstract: Four 2,4-D concentrations along with three embryo developmental stages were tested to determine the optimum morphogenesis of wheat immature embryo culture. Concentration of 2 mg L -1 2,4-D was found to be optimum level for morphogenesis which was a good indicator of embryogenesis. Compact and nodular calli were observed in first and second developmental stages of 2 mg L 1 2,4-D concentration. In addition, five callus initiation media were tested to determine effects of medium constituents on somatic embryogenesis of wheat (Triticum aestivum L.). The highest embryogenic callus formation was observed on MS+B5 medium (98.3%). Therefore, first and second developmental stages of 2 mg L -1 2,4-D concentration along with MS+B5 medium is suitable for embryogenic callus production in wheat.