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Journal of Biological Sciences

Year: 2002 | Volume: 2 | Issue: 6 | Page No.: 399-402
DOI: 10.3923/jbs.2002.399.402
Isolation and Molecular Characterization of Xylanase-Producing Bacteria from Drain Water
Narayan Roy and Md. Zainul Abedin

Abstract: The xylanase-producing bacterial strains were isolated from drain water. The strains were isolated on xylan-agar media and screening was carried out by β-xylanolysis method. The strains were further confirmed by DNS-method. The isolated strains were identified as Aeromonas on the basis of catalase, oxidase, gram staining and morphological cultural characteristics as well as by different biochemical tests. Multi-drug resistant of these strains were confirmed by antibiotic spread plate method. Disc diffusion method and antibiotic spread plate method showed the same multi-drug resistant pattern. Plasmid DNA was isolated from maximum xylanase-producing strain. Agarose gel electrophoresis of the isolated plasmid DNA in 0.8% agarose showed the presence of only one 30 kb plasmid. The plasmid DNA was transformed into nonxylanase-producing sensitive E. coli LE392. The transformants showed xylanase activity as well as multi-drug resistance. Xylanase activity and multi-drug resistance in Aeromonas strain was proposed to be plasmid mediated.

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How to cite this article
Narayan Roy and Md. Zainul Abedin, 2002. Isolation and Molecular Characterization of Xylanase-Producing Bacteria from Drain Water. Journal of Biological Sciences, 2: 399-402.

Keywords: Aeromonas sp, plasmid, dultridrud resistant and xylanase

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