Abstract: An investigation was undertaken to study the effect of different levels of chemical and organic fertilizers on the growth, yield and protein content of two wheat varieties. Two varieties of wheat were tested. Aghrani and Kanchan with five levels of chemical and organic fertilizer viz. F1 (control), F2, F3, F4 and F5. Urea was applied in 2 splits in F1 and in 3 splits in F2 to F4 as per recommendation. In F5, it was applied in 4 equal splits. In F1, inorganic fertilizers were applied @ 100 kg N, 36 kg P, 25 kg K and 20 kg S/ha. In F2 to F5, fertilizers were applied @ 120 kg N, 27 kg P, 83 kg K, 30 kg S and 4 kg Zn/ha. Grain Yield and protein content was higher in Kanchan than Aghrani variety. Among the different levels of fertilizer application, F5 had superior performance on all the parameters, whereas, F1 had the most inferior effect on all the parameters including grain and straw yields. The yield, yield contributing characters and protein content of wheat increased with increase in the levels inorganic fertilizers, addition of composts with fertilizer and also by increasing split application of nitrogen.