Abstract: Physico-chemical and biological study of three springs were carried out, which are located on the Lakki hills, near Lakki Shah Saddar Railway station, District Dadu. Two of these are euthermal (40-42°C) with significant evolution of hydrogen sulphide (135-152 mg/L) with high electrical conductivity 13000-14300 μS/cm mostly because of sodium chloride and pH 6.35-6.85 at the source. The third spring is chliarothermal having water temperature of 18-30°C and is a fresh water spring and forms seepage pool with pH 7.15, total dissolved solids (TDS) 1088 mg/L and electrical conductivity 1700μS/cm. The study revealed the occurrence of 43 spp. of Cyanophyta, 25 spp. Chlorophyta & 5 spp. Bacillarophyta in all the three springs, Chara zeylanica was present in fresh water spring, Lamprothamnium succinctum, Phragmites communis, Typha domingensis along with zooplankton, insects, Mollusca and Fishes, Barbus tictio were found present at the end of the channel of the two hot springs.