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Journal of Biological Sciences

Year: 2001 | Volume: 1 | Issue: 7 | Page No.: 541-547
DOI: 10.3923/jbs.2001.541.547
Effect of Some Herbicides as Chemical Pollutants on the Activity of Some Enzymes, Pigments Content and Nucleic Acids Level of Lupinus termis Cotyledons
H. M. El-Shora

Abstract: Two herbicides clomazone and metazachlor were non-competitively inhibited the activities of chorismate mutase (CM, EC 5.4.99.5), prephenate dehydratase (PD, EC 5.2.1.51), acetohydroxyacid synthase (AHAS, 4.1.3.18) and phenylalanine ammonia-lyase (PAL, 4.3.1.5) in cotyledons of Lupinus termis. Inhibition was dependent on the type and concentration of each individual herbicide. L-tryptophan and its analog 5-methyltryptophan activated CM and PD while L-tyrosine, m-fluorotyrosine, p-fluorophenylalanine and L-phenylalanine inhibited both enzymes particularly in herbicide-treated seedlings. PAL was inhibited by phenylpropanoid intermediates involved in flavonol and anthocyanin biosynthetic pathway particularly p-coumaric acid, naringenin and quercetin. PAL inhibition by these intermediates was more pronounced in cotyledons of herbicide-treated seedlings. The levels of chlorophyll a, chlorophyll b, carotenoids, anthocyanin and nucleic acids were decreased by the two tested herbicides.

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How to cite this article
H. M. El-Shora , 2001. Effect of Some Herbicides as Chemical Pollutants on the Activity of Some Enzymes, Pigments Content and Nucleic Acids Level of Lupinus termis Cotyledons. Journal of Biological Sciences, 1: 541-547.

Keywords: pigments, enzyme inhbition, Luplinus termis, metazachlor, Coomazone and nucleic acids

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