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Journal of Biological Sciences

Year: 2001 | Volume: 1 | Issue: 12 | Page No.: 1138-1140
DOI: 10.3923/jbs.2001.1138.1140
Varietal Response of Lycopersicon esculentum L. to Callogenesis and Regeneration
Zubeda Chaudary, Imran Feroz, Waseem Ahmed , Hamid Rashid, Bushra Mirza and Azra Quraishi

Abstract: The varietal response of Feston and Nagina was tested using hypocotyl and leaf disc as explant source. The callus formation was achieved on MS medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and on MS medium supplemented with Napthalene Acetic Acid (NAA) and Benzylamino Purine (BAP) from both explant sources. Shoot formation from calli of Feston and Nagina were successfully obtained on different hormonal levels of BAP & NAA on MS medium. Maximums shoot percentages of calli derived from hypocotyl (56.00%) and leaf disc (42.30%) was achieved on MS medium with 0.05mg/l IAA and 4.0mg/l BAP for Feston. For Nagina, maximum shoot percentage was obtained from leaf explant (39.13%) and hypocotyl (32.00%) on similar medium. Shoot regeneration produced 3.137 and 2.353 mean number of shoots/explants for hypocotyl and leaf disc of Feston whereas Nagina responded with 2.187 and 1.783 mean number of shoots/explant for leaf disc and hypocotyl respectively.

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How to cite this article
Zubeda Chaudary, Imran Feroz, Waseem Ahmed , Hamid Rashid, Bushra Mirza and Azra Quraishi , 2001. Varietal Response of Lycopersicon esculentum L. to Callogenesis and Regeneration. Journal of Biological Sciences, 1: 1138-1140.

Keywords: Tomato, hypocotyls, leaf disc and callus regeneration

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