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Journal of Applied Sciences

Year: 2002 | Volume: 2 | Issue: 6 | Page No.: 658-660
DOI: 10.3923/jas.2002.658.660
Assay of Cyanide in Biological Materials Using 2,2-Dihydroxy-1,3-Indanedione
Drochioiu, G. and Mangalagiu, I.

Abstract: The paper describes a novel cyanide assay in biological samples based on its reaction with 2,2-dihydroxy-1, 3-indanedione. Being very simple, accurate, fast, selective and sensitive, the method is useful in the determination of as little as 0.025 μg ml-1 CN – in biological samples. The molar absorptivity was found to be 4.8 x 104 | mole-1 cm-1 for 1.0 μg ml-1. The oxidizers such as oxygen, bromine, chlorine and even copper (II) and silver (I) ions interfere seriously reacting with the 2H indene derivative formed. The coefficient of variation for replicate analyses is 2.4%. The procedure could be applied in forensic studies and to follow the cyanide accumulation in smokers.

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How to cite this article
Drochioiu, G. and Mangalagiu, I., 2002. Assay of Cyanide in Biological Materials Using 2,2-Dihydroxy-1,3-Indanedione. Journal of Applied Sciences, 2: 658-660.

Keywords: cyanide, 2, 2-dihydroxy, 1-33indanediione, biological materials and spectrohotometic assay

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