Effect of Fermented Ginkgo biloba and Camelia sinensis-Based Probiotics on Growth Performance, Immunity and Caecal Microbiology in Broilers
Abstract:
Present study was conducted to evaluate the effects of dietary supplementation with fermented Ginkgo biloba and Camelia sinensis-based probiotics on growth performance, immunity and caecal microbiology in broilers. A total of three hundred twenty day old Ross broilers were randomly allocated based on completely randomized design into five treatments with eight replications (eight birds per replicate). Dietary treatments included: (1) Control (basal diet); (2) FGB1 = basal diet+0.2% fermented Ginkgo biloba probiotics; (3) FGB2 = basal diet+0.4% fermented Ginkgo biloba probiotics; (4) FCS1 = basal diet+0.2% fermented Camelia sinensis probiotics, (5) FCS2 = basal diet+0.4% fermented Camelia sinensis probiotics. Results of the present study elucidated that average daily gain was higher in FGB2 than FCS1 and FCS2 (p<0.05) during starter period; where feed intake was unaffected after dietary supplementation during starter, finisher and overall period (p>0.05). However, feed conversion ratio was improved in FGB2 during starter period (p<0.05), as well as in FGB1, FGB2, FCS1 and FCS2 during finisher and overall period relative to control (p<0.05). In addition, serum immunoglobulin was elevated in the FGB and FCS supplemented group compared to control (p<0.05). Moreover, dietary supplementation of FGB and FCS significantly suppressed caecal pathogenic E. coli (p<0.05). To sum up, dietary FGB and FCS can be utilized as potential feed additives in broiler nutrition with significant improvement in the growth performance, immunity and suppression of pathogenic caecal E. coli. Further detailed study is required on mechanism and meat quality analysis in broilers.
How to cite this article
Ye-Jin Kim, A.B.M. Rubayet Bostami, M.M. Islam, Hong Seok Mun, S.Y. Ko and Chul-Ju Yang, 2016. Effect of Fermented Ginkgo biloba and Camelia sinensis-Based Probiotics on Growth Performance, Immunity and Caecal Microbiology in Broilers. International Journal of Poultry Science, 15: 62-71.
REFERENCES
Aattouri, N., M. Bouras, D. Tome, A. Marcos and D. Lemonnier, 2002. Oral ingestion of lactic-acid bacteria by rats increases lymphocyte proliferation and interferon-gamma production. Br. J. Nutr., 87: 367-373.
PubMed Direct Link
Abuelsaad, A.S.A., I. Mohamed, G. Allam and A.A. Al-Solumani, 2013. Antimicrobial and immunomodulating activities of hesperidin and ellagic acid against diarrheic Aeromonas hydrophila in a murine model. Life Sci., 93: 714-722.
CrossRef Direct Link
Ahmed, S.T., H.S. Mun, M.M. Islam, S.S. Kim, J.A. Hwang, Y.J. Kim and C.J. Yang, 2014. Effects of Citrus junos by-products fermented with multistrain probiotics on growth performance, immunity, caecal microbiology and meat oxidative stability in broilers. Br. Poult. Sci., 55: 540-547.
CrossRef Direct Link
Alcicek, A., M. Bozkurt and M. Cabuk, 2004. The effect of a mixture of herbal essential oils, an organic acid or a probiotic on broiler performance. S. Afr. J. Anim. Sci., 34: 217-222.
Direct Link
Al-Masad, M., 2012. Effects of vitamin C and zinc on broilers performance of immunocompetence under heat stress. Asian J. Anim. Sci., 6: 76-84.
CrossRef Direct Link
AOAC., 2000. Official Methods of Analysis of the Association of Official Analytical Chemists. 17th Edn., AOAC International, Washington, DC USA
Bedford, M., 2000. Removal of antibiotic growth promoters from poultry diets: Implications and strategies to minimise subsequent problems. World's Poult. Sci. J., 56: 347-365.
CrossRef Direct Link
Borris, R.P., 1996. Natural products research: Perspectives from a major pharmaceutical company. J. Ethnopharmacol., 51: 29-38.
CrossRef Direct Link
Bostami, A.B.M., S.T. Ahmed, M.M. Islam, H.S. Mun, S.Y. Ko, S.S. Kim and C.J. Yang, 2015. Growth performance, fecal noxious gas emission and economic efficacy in broilers fed fermented pomegranate byproducts as residue of fruit industry. Int. J. Adv. Res., 3: 102-114.
Direct Link
Cao, B.H., Y. Karasawa and M. Guo, 2005. Effects of green tea polyphenols and fructo-oligosaccharides in semi-purified diets on broilers' performance and caecal microflora and their metabolites. Asian-Aust. J. Anim. Sci., 18: 85-89.
Direct Link
Cao, F.L., X.H. Zhang, W.W. Yu, L.G. Zhao and T. Wang, 2012. Effect of feeding fermented Ginkgo biloba leaves on growth performance, meat quality and lipid metabolism in broilers. Poult. Sci., 91: 1210-1221.
CrossRef Direct Link
Cho, J.H., Z.F. Zhang and I.H. Kim, 2013. Effects of fermented grains as raw cereal substitutes on growth performance, nutrient digestibility, blood profiles and fecal noxious gas emission in growing pigs. Livestock Sci., 154: 131-136.
CrossRef Direct Link
Cowan, M.M., 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev., 12: 564-582.
CrossRef PubMed Direct Link
Ehrmann, M.A., P. Kurzak, J. Bauer and R.F. Vogel, 2002. Characterization of lactobacilli towards their use as probiotic adjuncts in poultry. J. Applied Microbiol., 92: 966-975.
CrossRef Direct Link
Erdogan, Z., S. Erdogan, O. Aslantas and S. Celik, 2010. Effects of dietary supplementation of synbiotics and phytobiotics on performance, caecal coliform population and some oxidant/antioxidant parameters of broilers. J. Anim. Physiol. Anim. Nutr., 94: E40-E48.
CrossRef Direct Link
Feng, J., X. Liu, Z.R. Xu, Y.P. Lu and Y.Y. Liu, 2007. The effect of aspergillus oryzae fermented soybean meal on growth performance, digestibility of dietary components and activities of intestinal enzymes in weaned piglets. Anim. Feed Sci. Technol., 134: 295-303.
CrossRef Direct Link
Fulton, R.M., B.N. Nersessian and W.M. Reed, 2002. Prevention of Salmonella enteritidis infection in commercial ducklings by oral chicken egg-derived antibody alone or in combination with probiotics. Poult. Sci., 81: 34-40.
CrossRef Direct Link
Grashorn, M.A., 2010. Use of phytobiotics in broiler nutrition-an alternative to infeed antibiotics? J. Anim. Feed Sci., 19: 338-347.
Direct Link
Hara-Kudo, Y., A. Yamasaki, M. Sasaki, T. Okubo and Y. Minai et al., 2005. Antibacterial action on pathogenic bacterial spore by green tea catechins. J. Sci. Food Agric., 85: 2354-2361.
CrossRef Direct Link
Hashemi, S.R., I. Zulkifli, M. Hair Bejo, A. Farida and M.N. Somchit, 2008. Acute toxicity study and phytochemical screening of selected herbal aqueous extract in broiler chickens. Int. J. Pharmacol., 4: 352-360.
CrossRef Direct Link
Heres, L., B. Engel, F. van Knapen, J.A. Wagenaar and B.A.P. Urlings, 2003. Effect of fermented feed on the susceptibility for Campylobacter jejuni colonisation in broiler chickens with and without concurrent inoculation of Salmonella enteritidis. Int. J. Food Microbiol., 87: 75-86.
CrossRef Direct Link
Hong, K.J., C.H. Lee and S.W. Kim, 2004. Aspergillus oryzae GB-107 Fermentation improves nutritional quality of food soybeans and feed soybean meals. J. Med. Food, 7: 430-435.
CrossRef PubMed Direct Link
Hossain, M.E., G.M. Kim, S.K. Lee and C.J. Yang, 2012. Growth performance, meat yield, oxidative stability and fatty acid composition of meat from broilers fed diets supplemented with a medicinal plant and probiotics. Asian-Aust. J. Anim. Sci., 25: 1159-1168.
CrossRef Direct Link
Hsu, M.F. and B.H. Chiang, 2009. Effect of Bacillus subtilis natto-fermented Radix astragali on collagen production in human skin fibroblasts. Process Biochem., 44: 83-90.
CrossRef Direct Link
Izumi, T., M.K. Piskula, S. Osawa, A. Obata and K. Tobe et al., 2000. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J. Nutr., 130: 1695-1699.
PubMed Direct Link
Jun, K.D., H.J. Kim, K.H. Lee, H.D. Paik and J.S. Kang, 2002. Characterization of bacillus polyfermenticus SCD as a probiotic. Korean J. Microbiol. Biotech., 30: 359-366.
Kanatt, S.R., R. Chander and A. Sharma, 2010. Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products. Int. J. Food Sci. Technol., 45: 216-222.
CrossRef Direct Link
Karaoglu, M. and H. Durdag, 2005. The influence of dietary probiotic (Saccharomyces cerevisiae) supplementation and different slaughter age on the performance, slaughter and carcass properties of broilers. Int. J. Poult. Sci., 4: 309-316.
CrossRef Direct Link
Khalaji, S., M. Zaghari, K.H. Hatami, S. Hedari-Dastjerdi, L. Lotfi and H. Nazarian, 2011. Black cumin seeds, Artemisia leaves (Artemisia sieberi) and Camellia L. plant extract as phytogenic products in broiler diets and their effects on performance, blood constituents, immunity and cecal microbial population. Poult. Sci., 90: 2500-2510.
CrossRef PubMed Direct Link
Khan, N. and H. Mukhtar, 2013. Tea and health: Studies in humans. Curr. Pharm. Des., 19: 6141-6147.
CrossRef Direct Link
Kim, D.W., S.H. Kim, D.J. Yu, G.H. Kang and J.H. Kim et al., 2007. Effects of single or mixed supplements of plant extract, fermented medicinal plants and lactobacillus on growth performance in broilers. Korean J. Poult. Sci., 34: 187-196.
CrossRef Direct Link
Kim, K.S., G.M. Kim, M.E. Hossain, S.W. Park and C.J. Yang, 2010. Effect of Alisma canaliculatum, Viscum albumas and Cornus officinalis probiotics feed additives on growth performance and immunity in growing pigs. Proceeding of the Annual Congress of Korean Society of Animal Sciences and Technology, (ACKSAST'10), Jinju, South Korea, pp: 217-217.
Kyriakis, S.C., V.K. Tsiloyiannis, J. Vlemmas, K. Sarris, A.C. Tsinas, C. Alexopoulos and L. Jansegers, 1999. The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets. Res. Vet. Sci., 67: 223-228.
CrossRef Direct Link
Le Bars, P.L., M.M. Katz, N. Berman, T.M. Itil, A.M. Freedman and A.F. Schatzberg, 1997. A placebo-controlled, double-blind, randomized trial of an extract of ginkgo biloba for dementia. J. Am. Med. Assoc., 278: 1327-1332.
CrossRef Direct Link
Mandalari, G., R.N. Bennett, G. Bisignano, D. Trombetta and A. Saija et al., 2007. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry. J. Appl. Microbiol., 103: 2056-2064.
CrossRef PubMed Direct Link
Mountzouris, K.C., P. Tsirtsikos, E. Kalamara, S. Nitsch, G. Schatzmayr and K. Fegeros, 2007. Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult. Sci., 86: 309-317.
CrossRef PubMed Direct Link
Mukhatar, H. and N. Ahmad, 1999. Mechanism of cancer chemopreventive activity of green tea. Proc. Soc. Exp. Biol. Med., 220: 234-238.
PubMed Direct Link
Ng, C.C., C.Y. Wang, Y.P. Wang, W.S. Tzeng and Y.T. Shyu, 2011. Lactic acid bacterial fermentation on the production of functional antioxidant herbal Anoectochilus formosanus hayata. J. Biosci. Bioeng., 111: 289-293.
CrossRef Direct Link
Nishida, T., B. Eruden, K. Hosoda, H. Matsuyama, K. Nakagawa, T. Miyazawa and S. Shioya, 2006. Effects of green tea (Camellia sinensis) waste silage and polyethylene glycol on ruminal fermentation and blood components in cattle. Asian-Aust. J. Anim. Sci., 19: 1728-1736.
Direct Link
Patterson, J.A. and K.M. Burkholder, 2003. Application of prebiotics and probiotics in poultry production. Poult. Sci., 82: 627-631.
CrossRef PubMed Direct Link
Rajan, S., S. Mahalakshmi, V.M. Deepa, K. Sathya, S. Shajitha and T. Thirunalasundari, 2011. Antioxidant potentials of Punica granatum fruit rind extracts Int. J. Pharm. Pharm. Sci., 3: 82-88.
Direct Link
Sarkar, M.S.K., S.Y. Ko, G.M. Kim and C.J. Yang, 2010. Effects of Camellia sinensis and mixed probiotics on the growth performance and body composition in broiler. J. Med. Plant. Res., 4: 546-550.
Direct Link
SAS., 2003. SAS/STAT Software. Ver. 9.1, SAS Institute Inc., Cary, NC., USA
Shimizu, T., I. Mifuchi and T. Yokokura, 1981. Mitogenic effect of lactobacilli on murine lymphocytes. Chem. Pharmaceut. Bull., 29: 3731-3734.
Sohaib, M., M.S. Butt, M.A. Shabbir and M. Shahid, 2015. Lipid stability, antioxidant potential and fatty acid composition of broilers breast meat as influenced by quercetin in combination with α-tocopherol enriched diets. Lipids Health Dis., Vol. 14.
CrossRef
Terada, A., H. Hara, S. Nakajyo, H. Ichikawa and Y. Hara et al., 1993. Effect of supplements of tea polyphenols on the caeeal flora and caeeal metabolites of chicks. Microb. Ecol. Health Dis., 6: 3-9.
CrossRef Direct Link
van Beek, T.A. and P. Montoro, 2009. Chemical analysis and quality control of Ginkgo biloba leaves, extracts and phytopharmaceuticals. J. Chromatogr. A, 1216: 2002-2032.
CrossRef Direct Link
Wald, C., 2003. Gewurze and Co.-eine ubersicht. Lohmann Inform., 3: 7-11.
Direct Link
Yang, C.J., I.Y. Yang, D.H. Oh, I.H. Bae and S.G. Cho et al., 2003. Effect of green tea by-product on performance and body composition in broiler chicks. Asian-Australasian J. Anim. Sci., 16: 867-872.
CrossRef Direct Link
Yang, X.Y., Y.X. Lin and Y. Li, 2008. Effect of Ginkgo biloba extract on growth performance,slaughter performance and immune index in broilers. J. Fujian Agric. For. Univ. (Nat. Sci. Edn.), 3: 16-16.
Direct Link
Zhang, X., F. Cao, Z. Sun, W. Yu, L. Zhao, G. Wang and T. Wang, 2012. Effect of feeding Aspergillus niger-fermented Ginkgo biloba-leaves on growth, small intestinal structure and function of broiler chicks. Livestock Sci., 147: 170-180.
CrossRef Direct Link
Zhang, W.F., D.F. Li, W.Q. Lu and G.F. Yi, 2003. Effects of isomalto-oligosaccharides on broiler performance and intestinal microflora. Poult. Sci., 82: 657-663.
CrossRef PubMed Direct Link
Zhao, J., Y. Su, A. Chen, H. Yuan, L. Liu and W. Wu, 2011. Effect of ginkgo leaf parenteral solution on blood and cochlea antioxidant and immunity indexes in OM rats. Molecules, 16: 10433-10442.
CrossRef Direct Link
Zheng, G., L.Z. Yan, J.C. Vederas and P. Zuber, 1999. Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin. J. Bacteriol., 181: 7346-7355.
PubMed Direct Link
Zhou, H., C. Wang, J. Ye, H. Chen and R. Tao, 2015. Effects of dietary supplementation of fermented Ginkgo biloba L. residues on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets. Anim. Sci. J., 86: 790-799.
CrossRef Direct Link
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