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International Journal of Poultry Science

Year: 2015 | Volume: 14 | Issue: 1 | Page No.: 1-12
DOI: 10.3923/ijps.2015.1.12
Proteomic Analysis of Mycoplasma gallisepticum Vaccine Strain F
S.D. Collier, S.L. Branton, J.D. Evans, S.A. Leigh, X.F. Wan, A.M. Cooksey, H.A. Olanrewaju and G.T. Pharr

Abstract: The persistence and displacement abilities of the Mycoplasma gallisepticum vaccine strain F (F-strain) are well documented. Understanding the mechanism(s) of colonization and persistence of F-strain will aid in the current intervention strategies to diagnose and control MG infections in poultry. In the present study, phase partition and liquid chromatography along with electrospray mass spectrometry were used to evaluate the proteome of F-strain. A total of 586, 478 and 339 proteins were recognized from whole cell lysate (total protein), aqueous (cytosolic) and detergent phases (membrane), respectively. The proteins identified with the database searches were then grouped into three categories: (1) proteins from the membrane phase and found in the total proteins (TM); (2) proteins from the cytosolic phase and found in the total proteins (TC) and (3) proteins derived from the membrane and cytosolic phases and found in the total proteins (TMC). There were a total of 93 (33 as putative membrane proteins predicted by SOSUI), 207(13) and 79(6) proteins in the TM, TC and TMC, respectively. The identified proteins were distributed among John Craig Venter Institute (JCVI) categories, with the majority predicted to be involved in protein synthesis. Investigation of the Mycoplasma gallisepticum F-strain proteome may aid in the identification and characterization of F-strain proteins that are important in host colonization.

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How to cite this article
S.D. Collier, S.L. Branton, J.D. Evans, S.A. Leigh, X.F. Wan, A.M. Cooksey, H.A. Olanrewaju and G.T. Pharr, 2015. Proteomic Analysis of Mycoplasma gallisepticum Vaccine Strain F. International Journal of Poultry Science, 14: 1-12.

Keywords: Mycoplasma gallisepticum, F-strain, mass spectrometry and proteomics

REFERENCES
  • Abd-El-Motelib, T.Y. and S.H. Kleven 1993. A comparative study of Mycoplasma gallisepticum vaccines in young chickens. Avian Dis., 37: 981-987.
    CrossRef    Direct Link    

  • Boguslavsky, S., D. Menaker, I. Lysnyansky, T. Liu and S. Levisohn et al., 2000. Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein. Infect. Immun., 68: 3956-3964.
    PubMed    Direct Link    

  • Branton, S.L., B.D. Lott, J.W. Deaton, J.M. Hardin and W.R. Maslin, 1988. F strain Mycoplasma gallisepticum vaccination of post-production-peak commercial Leghorns and its effect on egg and eggshell quality. Avian Dis., 32: 304-307.
    PubMed    Direct Link    

  • Branton, S.L., B.D. Lott, J.D. May, W.R. Maslin, C.R. Boyle and G.T. Pharr, 1997. The effects of F strain Mycoplasma gallisepticum, Mycoplasma synoviae and the dual infection in commercial layer hens over a 44-week laying cycle when challenged before beginning of lay. I. Egg production and selected egg quality parameters. Avian Dis., 41: 832-837.
    PubMed    Direct Link    

  • Chen, H., S. Yu, X. Shen, D. Chen, X. Qiu, C. Song and C. Ding, 2011. The Mycoplasma gallisepticum α-enolase is cell surface-exposed and mediates adherence by binding to chicken plasminogen. Microb. Pathog., 51: 285-290.
    CrossRef    PubMed    Direct Link    

  • Collier, S.D., G.T. Pharr, S.L. Branton, J.D. Evans , S.A. Leigh and B. Felfoldi, 2006. Initial proteomics analysis of differentially expressed proteins from Mycoplasma gallisepticum vaccine strains ts-11 and F detected by Western blotting. Int. J. Poult. Sci., 5: 330-336.
    CrossRef    Direct Link    

  • Davidsen, T., E. Beck, A. Ganapathy, R. Montgomery and N. Zafar et al., 2010. The comprehensive microbial resource. Nucleic Acids Res., 38: D340-D345.
    CrossRef    PubMed    Direct Link    

  • Demina, I.A., M.V. Serebryakova, V.G. Ladygina, M.A. Rogova, V.G. Zgoda, D.A. Korzhenevskyi and V.M. Govorun, 2009. Proteome of the bacterium Mycoplasma gallisepticum. Biochem., 74: 165-174.
    CrossRef    PubMed    Direct Link    

  • Evans, J.D., S.A. Leigh, J.L. Purswell, R. Jacob, E.D. Peebles, S.D. Collier and S.L. Branton, 2012. A comparative study of live attenuated F strain-derived Mycoplasma gallisepticum vaccines. Avian Dis., 56: 396-401.
    PubMed    Direct Link    

  • Evans, R.D. and Y.S. Hafez, 1992. Evaluation of a Mycoplasma gallisepticum strain exhibiting reduced virulence for prevention and control of poultry mycoplasmosis. Avian Dis., 36: 197-201.
    PubMed    Direct Link    

  • Fisunov, G.Y., D.G. Alexeev, N.A. Bazaleev, V.G. Ladygina and M.A. Galyamina et al., 2011. Core proteome of the minimal cell: comparative proteomics of three mollicute species. PLoS One.
    CrossRef    

  • Frey, M.L., R.P. Hanson and D.P. Andrson, 1968. A medium for the isolation of avian mycoplasmas. Am. J. Vet. Res., 29: 2163-2171.
    PubMed    Direct Link    

  • Furnkranz, U., K. Siebert-Gulle, R. Rosengarten and M.P. Szostak, 2013. Factors influencing the cell adhesion and invasion capacity of Mycoplasma gallisepticum. Acta Vet. Scand.,
    CrossRef    

  • Gingerich, E., 2002. Vaccine and vaccination for Mycoplasma Gallisepticum (MG). Proceedings of the Cornell Poultry Conference, (CPC'02), Ithaca, pp: 2-5.

  • Glew, M.D., G.F. Browning, P.F. Markham and I.D. Walker, 2000. pMGA phenotypic variation in Mycoplasma gallisepticum occurs in vivo and is mediated by trinucleotide repeat length variation. Infect. Immu., 68: 6027-6033.
    PubMed    Direct Link    

  • Hnatow, L.L., C.L.Jr. Keeler, L.L. Tessmer, K. Czymmek and J.E. Dohms, 1998. Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and Mycoplasma genitalium P32. Infect. Immun., 66: 3436-3442.
    PubMed    Direct Link    

  • Hudson, P., T.S. Gorton, L. Papazisi, K. Cecchini, S. Frasca Jr. and S.J. Geary, 2006. Identification of a virulence-associated determinant, dihydrolipoamide dehydrogenase (lpd), in Mycoplasma gallisepticum through in vivo screening of transposon mutants. Infect. Immun., 74: 931-939.
    CrossRef    PubMed    Direct Link    

  • Indikova, I., P. Much, L. Stipkovits, K. Siebert-Gulle, M.P. Szostak, R. Rosengarten and C. Citti, 2013. Role of the GapA and CrmA cytadhesins of Mycoplasma gallisepticum in promoting virulence and host colonization. Infect. Immun., 81: 1618-1624.
    CrossRef    PubMed    Direct Link    

  • Jenkins, C., S.J. Geary, M. Gladd and S.P. Djordjevic, 2007. The Mycoplasma gallisepticum OsmC-like protein MG1142 resides on the cell surface and binds heparin. Microbiology, 153: 1455-1463.
    CrossRef    Direct Link    

  • Jenkins, C., R. Samudrala, S.J. Geary and S.P. Djordjevic, 2008. Structural and functional characterization of an organic hydroperoxide resistance protein from Mycoplasma gallisepticum. J. Bacteriol., 190 : 2206-2216.
    CrossRef    Direct Link    

  • Kleven, S.H., H.H. Fan and K.S. Turner, 1998. Pen trial studies on the use of live vaccines to displace virulent Mycoplasma gallisepticum in chickens. Avian Dis., 42: 300-306.
    CrossRef    PubMed    Direct Link    

  • Kleven, S.H., M.I. Khan and R. Yamamoto, 1990. Fingerprinting of Mycoplasma gallisepticum strains isolated from multiple-age layers vaccinated with live F strain. Avian Dis., 34: 984-990.
    CrossRef    PubMed    Direct Link    

  • Ley, D.H., A.P. Avakian and J.E. Berkhoff, 1993. Clinical Mycoplasma gallisepticum infection in multiplier breeder and meat Turkeys caused by F strain: Identification by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, restriction endonuclease analysis and the polymerase chain reaction. Avian Dis., 37: 854-862.
    CrossRef    PubMed    Direct Link    

  • Ley, D.H., J.M. Mclaren, A.M. Miles, H.J. Barnes, S.H. Miller and G. Franz, 1997. Transmissibility of live Mycoplasma gallisepticum vaccine strains ts-11 and 6/85 from vaccinated layer pullets to sentinel poultry. Avian Dis., 41: 187-198.
    PubMed    Direct Link    

  • Lin, M.Y. and S.H. Kleven, 1984. Evaluation of attenuated strains of Mycoplasma gallisepticum as vaccines in young chickens. Avian Dis., 28: 88-99.
    CrossRef    PubMed    Direct Link    

  • Liu, L., V.S. Panangala and K. Dybvig, 2002. Trinucleotide GAA repeats dictate pMGA gene expression in Mycoplasma gallisepticum by affecting spacing between flanking regions. J. Bacteriol., 184: 1335-1339.
    CrossRef    PubMed    Direct Link    

  • Liu, T., M. Garcia, S. Levisohn, D. Yogev and S.H. Kleven, 2001. Molecular variability of the adhesin-encoding gene pvpA among Mycoplasma gallisepticum strains and its application in diagnosis. J. Clin. Microbiol., 39: 1882-1888.
    CrossRef    Direct Link    

  • Masukagami, Y., K.A. Tivendale, K. Mardani, I. Ben-Barak and P.F. Markham and G.F. Browning, 2013. The Mycoplasma gallisepticum virulence factor lipoprotein MslA is a novel polynucleotide binding protein. Infect. Immun., 81: 3220-3226.
    CrossRef    Direct Link    

  • May, M., L. Papazisi, T.S. Gorton and S.J. Geary, 2006. Identification of fibronectin-binding proteins in Mycoplasma gallisepticum strain R. Infect. Immun., 74: 1777-1785.
    CrossRef    PubMed    Direct Link    

  • Mudahi-Orenstein, S., S. Levisohn, S.J. Geary and D. Yogev, 2003. Cytadherence-deficient mutants of Mycoplasma gallisepticum generated by transposon mutagenesis. Infect. Immun., 71: 3812-3820.
    CrossRef    PubMed    Direct Link    

  • Papazisi, L., S. Frasca, M. Gladd, X. Liao, D.Yogev and S.J. Geary, 2002. GapA and CrmA coexpression is essential for Mycoplasma gallisepticum cytadherence and virulence. Infect. Immun., 70: 6839-6845.
    CrossRef    PubMed    Direct Link    

  • Papazisi, L., T.S. Gorton, G. Kutish, P.F. Markham and G.F. Browning et al., 2003. The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain Rlow. Microbiology, 149: 2307-2316.
    CrossRef    Direct Link    

  • Papazisi, L., K.E. Troy, T.S. Gorton, X. Liao and S.J. Geary, 2000. Analysis of cytadherence-deficient, GapA-negative Mycoplasma gallisepticum strain R. Infect. Immun., 68: 6643-6649.
    Direct Link    

  • Purswell, J.L., J.D. Evans, S.A. Leigh, S.D. Collier and H.A. Olanrewaju et al., 2012. Mycoplasma gallisepticum transmission: comparison of commercial F-strain vaccine versus layer complex-derived field strains in a tunnel ventilated house. Poult. Sci., 91: 3072-3079.
    CrossRef    PubMed    Direct Link    

  • Szczepanek, S.M., S. Frasca, V.L. Schumacher, X. Liao, M. Padula, S.P. Djordjevic and S.J. Geary, 2010. Identification of lipoprotein MslA as a neoteric virulence factor of Mycoplasma gallisepticum. Infect. Immun., 78: 3475-3483.
    CrossRef    Direct Link    

  • Szczepanek, S.M., E.R. Tulman, T.S. Gorton, X. Liao and Z. Lu et al., 2010. Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum. Infect. Immun., 78: 1760-1771.
    CrossRef    PubMed    Direct Link    

  • Tulman, E.R., X. Liao, S.M. Szczepanek, D.H. Ley, G.F. Kutish and S.J. Geary, 2012. Extensive variation in surface lipoprotein gene content and genomic changes associated with virulence during evolution of a novel North American house finch epizootic strain of Mycoplasma gallisepticum. Microbiology, 158: 2073-2088.
    CrossRef    PubMed    Direct Link    

  • Wan, X., S.L. Branton, L.A. Hanson and G.T. Pharr, 2004. Identification and initial characterization of a putative Mycoplasma gallinarum leucine aminopeptidase gene. Curr. Microbiol., 48: 32-38.
    PubMed    Direct Link    

  • Wan, X.F., S.L. Branton, S.D. Collier, J.D. Evans, S.A. Leigh and G.T. Pharr, 2010. Proteomics inference of genes involved in host adaptation of Mycoplasma gallinarum. Veterinary Microbiol., 145: 177-184.
    CrossRef    Direct Link    

  • Whithear, K.G., 1996. Control of Avian Mycoplasmoses by Vaccination. In: Animal Mycoplasmosis and Control, Nicolet, J. (Ed.). Vol. 15. Scientific and Technical Review-International Office of Epizootics, Paris, pp: 1527-1553

  • Whithear, K.G., E. Ghiocas, P.F. Markham and D. Marks, 1990. Examination of Mycoplasma gallisepticum isolates from chickens with respiratory disease in a commercial flock vaccinated with a living M. gallisepticum vaccine. Aust. Vet. J., 67: 459-460.
    CrossRef    Direct Link    

  • Winner, F., I. Markova, P. Much, A. Lugmair and K. Siebert-Gulle et al., 2003. Phenotypic switching in Mycoplasma gallisepticum hemadsorption is governed by a high-frequency, reversible point mutation. Infect. Immun., 71: 1265-1273.
    CrossRef    Direct Link    

  • Yoder, H.W. Jr., 1991. Mycoplasma Gallsepticum Infection. In: Diseases of Poultry, Calnek, B.W., H.J. Barnes, C.W. Beard and H.W. Yoder (Eds.)., Iowa State University Press, USA., pp: 198-212

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