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International Journal of Poultry Science

Year: 2014 | Volume: 13 | Issue: 4 | Page No.: 240-245
DOI: 10.3923/ijps.2014.240.245
Genetic Relatedness among Helicobacter pullorum Isolates from Broiler Chickens
Soe Soe Wai, A.A. Saleha, Z. Zunita, L. Hassan, A. Jalila and A.H. Shah

Abstract: Helicobacter pullorum, an enterohepatic helicobacter with the ability to colonize the intestine and liver of avian species, mice and humans, is increasingly recognized as a food borne zoonotic pathogen. The present study aimed to determine the genetic similarity among H. pullorum using pulsed field gel electrophoresis (PFGE) with SacII and SmaI restriction endonucleases (REs) digestion. The H. pullorum were isolated from broiler chickens in eight farms. Within-farm genetic profiling of isolates showed close relationship (>95%); however genetic diversity was observed between isolates from different farms. Digestion of genome with SacII yielded two clusters comprised 16 pulsotypes, 8-14 DNA bands with a molecular weight ranging from 40 to 400 kb whereas SmaI digested genome produced two clusters with 14 pulsotypes, 7 to 13 DNA bands with molecular weight ranging from 40 to 250 kb. The RE SacII showed a higher discriminatory power compared to SmaI. In conclusion, within-farm genetic similarity of isolates suggested the same source of clonal origin.

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How to cite this article
Soe Soe Wai, A.A. Saleha, Z. Zunita, L. Hassan, A. Jalila and A.H. Shah, 2014. Genetic Relatedness among Helicobacter pullorum Isolates from Broiler Chickens. International Journal of Poultry Science, 13: 240-245.

Keywords: Helicobacter pullorum, chickens, genetic relationship and PFGE

REFERENCES
  • Arcangioli, M.A., H. Aslan, F. Tardy, F. Poumarat and D. le Grand, 2011. The use of pulsed-field gel electrophoresis to investigate the epidemiology of Mycoplasma bovis in French calf feedlots. Vet. J., 192: 96-100.
    CrossRef    

  • Casswall, T.H., A. Nemeth, I. Nilsson, T. Wadstrom and H.O. Nilsson, 2010. Helicobacter species DNA in liver and gastric tissues in children and adolescents with chronic liver disease. Scand. J. Gastroentero., 45: 160-167.
    PubMed    

  • Ceelen, L.M., A. Decostere, K. van den Bulck, S.L. On, M. Baele, R. Ducatelle and F. Haesebrouck, 2006. Helicobacter pullorum in chickens, Belgium. Emerg. Infect. Dis., 12: 263-267.
    PubMed    

  • Dingle, K.E., F.M. Colles, D.R.A. Wareing, R. Ure and A.J. Fox et al., 2001. Multilocus sequence typing system for Campylobacter jejuni. J. Clin. Microbiol., 39: 14-23.
    CrossRef    PubMed    Direct Link    

  • Fox, J.G., 1997. The expanding genus of Helicobacter: Pathogenic and zoonotic potential. Semin. Gastrointest. Dis., 8: 124-141.
    PubMed    

  • Gibson, J.R., M.A. Ferrus, D. Woodward, J. Xerry and R.J. Owen, 1999. Genetic diversity in Helicobacter pullorum from human and poultry sources identified by an amplified fragment length polymorphism technique and pulsed-field gel electrophoresis. J. Applied Microbol., 87: 602-610.
    PubMed    Direct Link    

  • Hanninen, M.L., S. Pajarre, M.L. Klossner and H. Rautelin, 1998. Typing of human Campylobacter jejuni isolates in Finland by pulsed-field gel electrophoresis. J. Clin. Microbiol., 36: 1787-1789.
    PubMed    

  • Hume, M.E., R.B. Harvey, L.H. Stanker, R.E. Droleskey, T.E. Poole and H.B. Zhang, 2001. Genotypic variation among Arcobacter isolates from a farrow-to-finish swine facility. J. Food Prot., 64: 645-651.
    PubMed    

  • Hunter, P.R. and M.A. Gaston, 1988. Numerical index of the discriminatory ability of typing systems: An application of Simpson's index of diversity. J. Clin. Microbiol., 26: 2465-2466.
    Direct Link    

  • Jalava, K., M.C. de Ungria, J. O'Rourke, A. Lee, U. Hirvi and M.L. Hanninen, 1999. Characterization of Helicobacter felis by pulsed-field gel electrophoresis, plasmid profiling and ribotyping. Helicobacter, 4: 17-27.
    PubMed    

  • Levesque, S., E. Frost, R.D. Arbeit and S. Michaud, 2008. Multilocus sequence typing of Campylobacter jejuni isolates from humans, chickens, raw milk and environmental water in Quebec, Canada. J. Clin. Microbiol., 46: 3404-3411.
    CrossRef    Direct Link    

  • Lindmark, H., B. Harbom, L. Thebo, L. Andersson and G. Hedin et al., 2004. Genetic characterization and antibiotic resistance of Campylobacter jejuni isolated from meats, water and humans in Sweden. J. Clin. Microbiol., 42: 700-706.
    CrossRef    

  • Manfreda, G., A. Parisi, A. Lucchi, R.G. Zanoni and A. de Cesare, 2011. Prevalence of Helicobacter pullorum in conventional, organic and free-range broilers and typing of isolates. Applied Environ. Microbiol., 77: 479-484.
    CrossRef    

  • Michaud, S., R.D. Arbeit and C. Gaudreau, 2001. Molecular strain typing of Campylobacter jejuni by pulsed-field gel electrophoresis in a single day. Can. J. Microbiol., 47: 667-669.
    CrossRef    

  • Miller, K.A., L.L. Blackall, J.K. Miflin, J.M. Templeton and P.J. Blackall, 2006. Detection of Helicobacter pullorum in meat chickens in Australia. Aust. Vet. J., 84: 95-97.
    CrossRef    

  • Mohamed, M.A., R.S. Ibrahim, M.A. Shahata and E.R.M. El-Refaie, 2010. Helicobacter pullorum among poultry in assiut-Egypt: Genetic characterization, virulence and MIC. Int. J. Poult. Sci., 9: 521-526.
    CrossRef    Direct Link    

  • Nebbia, P., C. Tramuta, M. Ortoffi, E. Bert, S. Cerruti Sola and P. Robino, 2007. Identification of enteric Helicobacter in avian species. Schweizer Arch. Tierheilkunde, 149: 403-407.
    CrossRef    PubMed    

  • Oyarzabal, O.A., S. Backert, L.L. Williams, A.J. Lastovica and R.S. Miller et al., 2008. Molecular typing, serotyping and cytotoxicity testing of Campylobacter jejuni strains isolated from commercial broilers in Puerto Rico. J. Applied Microbiol., 105: 800-812.
    PubMed    

  • Rivas, L., N. Fegan and P. Vanderlinde, 2004. Isolation and characterisation of Arcobacter butzleri from meat. Int. J. Food Microbiol., 91: 31-41.
    CrossRef    

  • Rocha, M., P. Avenaud, A. Menard, B. le Bail and C. Balabaud et al., 2005. Association of Helicobacter species with hepatitis C cirrhosis with or without hepatocellular carcinoma. Gut, 54: 396-401.
    CrossRef    Direct Link    

  • Sails, A.D., B. Swaminathan and P.I. Fields, 2003. Utility of multilocus sequence typing as an epidemiological tool for investigation of outbreaks of gastroenteritis caused by Campylobacter jejuni. J. Clin. Microbiol., 41: 4733-4739.
    PubMed    

  • Saunders, K.E., K.J. McGovern and J.G. Fox, 1997. Use of pulsed-field gel electrophoresis to determine genomic diversity in strains of Helicobacter hepaticus from geographically distant locations. J. Clin. Microbiol., 35: 2859-2863.
    PubMed    

  • Taylor, D.E., N. Chang, N.S. Taylor and J.G. Fox, 1994. Genome conservation in Helicobacter mustelae as determined by pulsed-field gel electrophoresis. FEMS Microbiol., 118: 31-36.
    PubMed    

  • Taylor, D.E., M. Eaton, N. Chang and S.M. Salama, 1992. Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level. J. Bacteriol., 174: 6800-6806.
    Direct Link    

  • Tenover, F.C., R.D. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing and B. Swaminathan, 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J. Clin. Microbiol., 33: 2233-2239.
    PubMed    

  • Varon, C., A. Duriez, P. Lehours, A. Menard and S. Laye et al., 2009. Study of Helicobacter pullorum proinflammatory properties on human epithelial cells in vitro. Gut, 58: 629-635.
    CrossRef    

  • Wassenaar, T.M., B. Geilhausen and D.G. Newell, 1998. Evidence of genomic instability in Campylobacter jejuni isolated from poultry. Applied Environ. Microbiol., 64: 1816-1821.
    Direct Link    

  • Wesley, I.V., 2001. Arcobacter and Helicobacter. In: Guide to Foodborne Pathogens, Labbe, G. and S. Garcin (Eds.). John Wiley and Sons, New York, pp: 23-34

  • Zanoni, R.G., S. Piva, M. Rossi, F. Pasquali, A. Lucchi, A. de Cesare and G. Manfreda, 2011. Occurrence of Helicobacter pullorum in turkeys. Vet. Microbiol., 149: 492-496.
    CrossRef    

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