Association of TLR4 Gene Genotype and Resistance Against Salmonella enteritidis Natural Infection in Kampung Chicken
Abstract:
Eggs of kampung chicken play an important role as substance
in jamu preparation
in Indonesia, mostly provided and consumed without cooking. Salmonella
free eggs become significant in producing the safe jamu
preparation and such eggs might
be produced by chickens which have high resistancy to this bacteria. One of
excellent markers showing resistance of chicken against Salmonella is
an active Toll-like Receptor 4 (TLR4) gene. TLR4 is a phagocytes cell surface
receptor that plays a role to recognize lipopolysaccaride (LPS) of gram negative
bacteria including Salmonella enteritidis. It is transcribed by TLR4
gene and conserved in the activation of the non-specific immune system. The
aim of the research was to prove how kampung chicken resistant against natural
infection of S. enteritidis, using TLR4 gene as marker. TLR4 gene was
genotyped in 50 kampung chickens with PCR-RFLP. Then biological assays of resistance
indicator were measured. The genotyping result on exon 2 (220 bp in size) identified
3 genotypes of TLR4 gene in kampung chicken: AA, AG and GG. Concentration of
leucocytes and their differentiation were not significantly different in AG
and GG genotype. The value of it from AA genotype was similar to them. There
was no S. enteritidis finding in blood and eggs produced by AA, AG, GG
chickens. Specific IgY to S. enteritidis was positively found in kampung
chickens serum and egg yolk. Concentration of specific IgY in kampung chicken
egg yolk was found very high. The study postulated that most likely kampung
chicken resistant to S. enteritidis natural infection.
How to cite this article
Niken Ulupi, Muladno ., Cece Sumantri and I. Wayan Teguh Wibawan, 2013. Association of TLR4 Gene Genotype and Resistance Against Salmonella enteritidis Natural Infection in Kampung Chicken. International Journal of Poultry Science, 12: 445-450.
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