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International Journal of Poultry Science

Year: 2010 | Volume: 9 | Issue: 10 | Page No.: 988-995
DOI: 10.3923/ijps.2010.988.995
Effects of Polyphenols Extracted from Tamarind (Tamarindus indica L.) Seed Coat on Body Weight, White Blood Cells, Bursa of Fabricius and NDV-HI Titer of Broilers under Chronic Heat Stress
T. Srikhun, W. Aengwanich and W. Kongbuntad

Abstract: The purpose of this experiment was to investigate the effects of polyphenols extracted from tamarind (Tamarindus indica L.) seed coat on total body weight, white blood cells, bursa of Fabricius and NDV-HI titer of broilers maintained in high environmental temperature. The broilers under study were divided into two main groups. Those in the first group were maintained at 26"2oC and the second group at 38"2oC. The broilers in the second group were subdivided into six sub-groups, each of which received polyphenols at 0, 100, 200, 300, 400 and 500 mg/kg in their diets. The results revealed that the body weights of the broilers in all groups were not different. The total white blood cell counts and lymphocyte counts of the broilers in all sub-groups of the second group were higher than that of the broilers in the first group (p<0.05). On day 21 of the experimental period, the total number of white blood cells and lymphocytes of the broilers in the first group was not different from those of the broilers in the first, second, fifth and sixth sub-groups of the second group (p>0.05). The relative bursa of Fabricius weight of the broilers in the first group and the third sub-group of the second group were not different (p>0.05). Furthermore, lesion scores of the bursa of Fabricius of the first group and the third and fourth sub-groups of the second group were not different (p>0.05). Moreover, NDV-HI titer of the broilers in the first group and all sub-groups of the second group were not also different (p>0.05). These phenomena indicated that polyphenols could increase the number of lymphocytes and relative bursa of Fabricius weight. On the other hand, the extract could decrease lesion scores of bursa of Fabricius in heat stressed broilers.

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How to cite this article
T. Srikhun, W. Aengwanich and W. Kongbuntad, 2010. Effects of Polyphenols Extracted from Tamarind (Tamarindus indica L.) Seed Coat on Body Weight, White Blood Cells, Bursa of Fabricius and NDV-HI Titer of Broilers under Chronic Heat Stress. International Journal of Poultry Science, 9: 988-995.

Keywords: Polyphenols, tamarind (Tamarindus indica L.), broiler, immunity and heat stress

REFERENCES
  • Aengwanich, W., S. Sridama, Y. Phasuk, T. Vongpralab, P. Pakdee, S. Katavetin and S. Simaraks, 2003. Effects of ascorbic acid on cell mediated, humoral immune system and pathophysiology of white blood cell in broilers under heat stress. Songklanakarin J. Sci. Technol., 25: 297-306.
    Direct Link    

  • Aengwanich, W., 2007. Comparative ability to tolerate heat between Thai indigenous chickens, Thai indigenous chickens crossbred and broilers by using heterophil/lymphocyte ratio. Pak. J. Biol. Sci., 10: 1840-1844.
    CrossRef    PubMed    Direct Link    

  • Aengwanich, W., 2008. Pathological changes and effect of ascorbic acid on lesion scores of bursa of Fabricius in broilers under chronic heat stress. Res. J. Vet. Sci., 1: 62-66.

  • Aengwanich, W., 2008. Comparative ability to tolerate heat between Thai indigenous chickens, Thai indigenous chickens crossbred and broilers by using percentage of lymphocyte. Int. J. Poult. Sci., 7: 1071-1073.
    CrossRef    Direct Link    

  • Aengwanich, W. and M. Suttajit, 2010. Effect of polyphenols extracted from Tamarind (Tamarindus indica L.) seed coat on physiological changes, heterophil/lymphocyte ratio, oxidative stress and body weight of broilers (Gallus domesticus) under chronic heat stress. Anim. Sci. J., 81: 264-270.
    CrossRef    PubMed    Direct Link    

  • Aengwanich, W., M. Suttajit, T. Srikhun and T. Boonsorn, 2009. Antibiotic effect of polyphenolic compound extracted from tamarind (Tamarindus indica L.) seed coat on productive performance of broilers. Int. J. Poult. Sci., 8: 749-751.
    CrossRef    Direct Link    

  • Aengwanich, W., M. Suttajit and N-A. Narkkong, 2009. Effects of polyphenols extracted from tamarind (Tamarindus indica L.) seed coat on differential white blood cell count in broilers (Gallus domesticus) exposed to high environmental temperature. Int. J. Poult. Sci., 8: 957-962.
    CrossRef    Direct Link    

  • Zahraa and H. Al-Ghamdi, 2008. Effects of commutative heat stress on immunoresponses in broiler chickens reared in closed system. Int. J. Poult. Sci., 7: 964-968.
    CrossRef    Direct Link    

  • Altan, O., A. Altan, M. Cabuk and H. Bayraktar, 2000. Effects of heat stress on some blood parameters in broilers. Turk. J. Vet. Anim. Sci., 24: 145-148.
    Direct Link    

  • Borges, S.A., J. Ariki, C.L. Martins and V.B.M. de Moraes, 1999. Potassium chloride supplementation in heat stressed broilers. Rev. Bras. de Zootec., 28: 313-319.

  • Borges, S.A., A.V.F. da Silva, A. Majorka, D.M. Hooge and K.R. Cummings, 2004. Physiological response of broiler chickens to heat stress and dietary electrolyte balance (sodium plus potassium minus chloride, milliequivalents perkilogram). Poult. Sci., 83: 1551-1558.
    Direct Link    

  • Campbell, T.W., 1995. Avian Hematology and Cytology. 2nd Edn., Iowa State University Press, Ames, Iowa, USA

  • Campo, J.L. and S.G. Davila, 2002. Changes in heterophil to lymphocyte ratios of heat-stressed chickens in response to dietary supplementation of several related stress agents. Arch. Geflugelk, 66: 80-84.
    Direct Link    

  • Compton, M.M., P.S. Gibbs and L.R. Swicegood, 1990. Glucocorticoid-mediated activation of DNA degradation in avian lymphocytes. Gen. Comp. Endocrinol., 80: 68-79.
    CrossRef    PubMed    Direct Link    

  • Cooper, M.A. and K.W. Washburn, 1998. The relationships of body temperature to weight gain, feed consumption and feed utilization in broilers under heat stress. Poult. Sci., 77: 237-242.
    PubMed    Direct Link    

  • Davis, A.K., D.L. Maney and J.C. Maerz, 2008. The use of leukocyte profiles to measure stress in vertebrates: A review for ecologists. Funct. Ecol., 22: 760-772.
    CrossRef    Direct Link    

  • Gu, L., M.A. Kelm, J.F. Hammerstone, Z. Zhang and G. Beecher et al., 2003. Liquid chromatographic/electrospray ionization mass spectrometric studies of procyanidins in foods. J. Mass Spectrometry, 38: 1272-1280.

  • Heckert, R.A., I. Estevez, E. Russek-Cohen and R. Pettit-Riley, 2002. Effects of density and perch availability on the immune status of broilers. Poult. Sci., 81: 451-457.
    CrossRef    PubMed    Direct Link    

  • Jain, N.C., 1993. Essentials of Veterinary Hematology. Lea and Febiger, Philadelphia, USA., pp: 7

  • Komutarin, T., S. Azadi, L. Butterworth, D. Keil, B. Chitsomboon, M. Suttajit and B.J. Meade, 2004. Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo. Food Chem. Toxicol., 42: 649-658.

  • Lien, R.J., J.B. Hess, S.R. McKee, S.F. Bilgili and J.C. Townsend, 2007. Effect of light intensity and photoperiod on live performance, heterophil-to-lymphocyte ratio and processing yields of broilers. Poult. Sci., 86: 1287-1293.
    PubMed    Direct Link    

  • Luna, L.T., 1968. Manual of Histologic Staining Methods of the Armed Force Institute of Pathology. McGraw-Hill Book Company, New York, pp: 1-39

  • Moberg, G.P. and J.A. Mench, 2000. The Biology of Animal Stress: Basic Principles and Implications for Animal Welfare. CAB International, New York

  • Mujahid, A., Y. Yoshiki, Y. Akiba and M. Toyomizu, 2005. Superoxide radical production in chicken skeletal muscle induced by acute heat stress. Poult. Sci., 84: 307-314.
    CrossRef    PubMed    Direct Link    

  • Naseem, S., M. Younus, B. Anwar, A. Ghafoor, A. Aslam and S. Akhter, 2005. Effect of ascorbic acid and acetylsalicylic acid supplementation on performance of broiler chicks exposed to heat stress. Int. J. Poult. Sci., 4: 900-904.
    CrossRef    Direct Link    

  • Pamok, S., W. Aengwanich and T. Komutrin, 2009. Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heat stressed broilers. J. Therm. Biol., 34: 353-357.
    CrossRef    

  • Puvadolpirod, S. and J.P. Thaxton, 2000. Model of physiological stress in chickens 1. Response parameters. Poult. Sci., 79: 363-369.
    CrossRef    Direct Link    

  • Puvadolpirod, S. and J.P. Thaxton, 2000. Model of physiological stress in chickens 3. Temporal patterns of response. Poult. Sci., 79: 377-382.
    CrossRef    PubMed    Direct Link    

  • Ritchie, B.W., G.J. Harrison and R.L. Harrison, 1994. Avian Medicine: Principle and Application. 6th Edn., Wingers Publishing, Inc., Florida

  • Sudjaroen, Y., R. Haubner, G. Wurtele, W.E. Hull and G. Erben et al., 2005. Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp. Food Chem. Toxicol., 43: 1673-1682.
    CrossRef    Direct Link    

  • Yalcin, S., S. Ozkan, G. Oktay, M. Cabuk, Z. Erbayraktar and S.F. Bilgili, 2004. Age-related effects of catching, crating and transportation at different season on core body temperature and physiological blood parameters in broilers. J. Applied Poult. Res., 13: 549-560.
    Direct Link    

  • Kahkonen, M.P., A.I. Hopia, H.J. Vuorela, J.P. Rauha, K. Pihlaja, T.S. Kujala and M. Heinonen, 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem., 47: 3954-3962.
    CrossRef    PubMed    Direct Link    

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