HOME JOURNALS CONTACT

International Journal of Poultry Science

Year: 2010 | Volume: 9 | Issue: 8 | Page No.: 756-760
DOI: 10.3923/ijps.2010.756.760
Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken
G. Goyal, V. Upmanyu, S. K. Singh, S.K. K. Shukla, S. Mehra, Vinay Kumar and Deepak Sharma

Abstract: Differential expression of IL-6 and IGF-II genes were studied in guinea fowl and broiler chicken using semi-quantitative analysis. A 219 bp fragment of IL-6 and 215 bp fragment of IGF-II were amplified in guinea fowl and broiler chicken using chicken specific primers. Semi-quantitative analysis revealed the adjusted Integral Density of 0.853 and 0.051 for IL-6 band in guinea fowl and broiler chicken respectively, revealing 16.62 fold higher IL-6 mRNA expression in LPS induced PBMCs from guinea fowl as compared to that from broiler. However, adjusted Integral Density of IGF-II band was 0.082 and 1.106 for IGF-II band in guinea fowl and broiler chicken respectively, which revealed 13.43 fold increase in IGF-II mRNA expression in LPS induced PBMCs in broiler chicken as compared to that in guinea fowl. Hence, guinea fowl showed higher expression of pro-inflammatory cytokine (IL-6) and lower expression of IGF-II in comparison to broiler chicken. These findings were as per expectation in view of much higher immuno-competence and lower growth rate in guinea fowl in comparison to chicken.

Fulltext PDF

How to cite this article
G. Goyal, V. Upmanyu, S. K. Singh, S.K. K. Shukla, S. Mehra, Vinay Kumar and Deepak Sharma, 2010. Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken. International Journal of Poultry Science, 9: 756-760.

Keywords: Differential expression, IL-6, IGF-II and guinea fowl

REFERENCES
  • Cunningham, C.H., 1971. Virologia Practica. 6th Edn., Acribia, Zaragoza, Pages: 260

  • Erickson, G.A., C.J. Mare, G.A. Gustafson, L.D. Miller, S.J. Proctor and E.A. Carbrey, 1977. Interaction between viscerotropic velogenic Newcastle disease virus and pet birds of six species I. Clinical and serological responses and viral excretion. Avian Dis., 21: 642-654.
    PubMed    

  • Hofstad, M.S., 1951. A quantitative study of Newcastle disease virus in tissues of infected chickens. Am. J. Vet. Res., 12: 334-339.
    PubMed    

  • Kaleta, E.F. and C. Baldauf, 1988. Newcastle Disease in Free-Living and Pets Birds. In: Newcastle Disease, Alexander, D.J. (Ed.). Kluwer Academic Publishers, Boston, MA., USA., pp: 197-246

  • Lendon, A.H., 1973. Australian Parrots in Field and Aviary. 2nd Edn., Angus and Robertson, Sydney, pp: 302-307

  • McFerran, J.B. and R. Nelson, 1971. Some properties of an avirulent Newcastle disease virus. Arch. Virol., 34: 64-74.
    CrossRef    Direct Link    

  • Winterfield, R.W., C.L. Goldman and E.H. Seadale, 1957. Newcastle disease immunization studies: Vaccination of chickens with B1, F and LaSota strains of Newcastle disease virus administered through the drinking water. Poult. Sci., 36: 1076-1088.

  • Aitken, I., W.H. Allan, R.M. Biggs, R.I. Gordon and F.T.W. Jordan, 1977. Newcastle Disease. In: Poultry Diseases, Gordon, R.F. (Ed.). Bailliere Tindall, London, pp: 81-94

  • Bayyari, G.R., W.E. Huff, N.C. Rath, J.M. Balog and L.A. Newberry et al., 1997. Effect of the genetic selection of turkeys for increased body weight and egg production on immune and physiological responses. Poult. Sci., 76: 289-296.
    PubMed    

  • Beccavin, C., B. Chevalier, L.A. Cogburn, J. Simon and M.J. Duclos, 2001. Insulin-like growth factors and body growth in chickens divergently selected for high or low growth rate. J. Endocrinol., 168: 297-306.
    PubMed    

  • Burnside, J. and L.A. Cogburn, 1992. Developmental expression of hepatic growth hormone receptor and insulin-like growth factor-I mRNA in the chicken. Mol. Cell. Endocrinol., 89: 91-96.
    PubMed    

  • Cheng, H.H. and S.J. Lamont, 2008. Genetics of Disease Resistance. In: Diseases of Poultry, Saif, Y.M., A. Fadly, J. Glisson, I. McDonald, L. Nolan and D. Swayne (Eds.). 12th Edn. Blackwell Publication, UK., pp: 59-72

  • Chiang, H.I., C.L. Swaggerty, M.H. Kogut, S.E. Dowd, X. Li, I.Y. Pevzner and H. Zhou, 2008. Gene expression profiling in chicken heterophils with Salmonella enteritidis stimulation using a chicken 44 K Agilent microarray. BMC Genom., 9: 526-526.
    CrossRef    

  • Duclos, M.J., 2005. Insulin-like growth factor-I (IGF-1) mRNA levels and chicken muscle growth. J. Physiol. Pharmacol., 56: 25-35.
    PubMed    

  • Ferro, P.J., C.L. Swaggerty, P. Kaiser, I.Y. Pevzner and M.H. Kogut, 2004. Heterophils isolated from chickens resistant to extra-intestinal Salmonella enteritidis infection express higher levels of pro-inflammatory cytokine mRNA following infection than heterophils from susceptible chickens. Epidemiol. Infect., 132: 1029-1037.
    PubMed    Direct Link    

  • Jones, J.I. and D.R. Clemmons, 1995. Insulin-like growth factors and their binding proteins: Biological actions. Endocrinol. Rev., 16: 3-34.
    PubMed    

  • Kajimoto, Y. and P. Rotwein, 1989. Structure and expression of a chicken insulin-like growth factor I precursor. Mol. Endocrinol., 3: 1907-1913.
    CrossRef    

  • Kikuchi, K., F.C. Buonomo, Y. Kajimoto and P. Rotwein, 1991. Expression of insulin-like growth factor-I during chicken development. Endocrinology, 128: 1323-1328.
    PubMed    

  • Kougt, M.H., E.D. Mcgrude, B.M. Hargis, D.E. Corrier and J.P. Deloach, 1994. Characterization of the pattern of inflammatory cell influx in chicks following the intraperitoneal administration of line Salmonella enteritidies-immune lymphokines. Poult. Sci., 74: 8-17.

  • Lamont, S.J., M.H. Pinard-van der Laan, A. Cahaner, J.J. van der Poel and H.K. Parmentier, 2003. Selection for Disease Resistance: Direct Selection on the Immune Response. In: Poultry Genetics, Breeding and Biotechnology, Muir, W.M. and S.E. Aggrey (Eds.). Chapter 22, CABI Publishing, Oxon, UK., ISBN-13: 9780851998459, pp: 399-418

  • Muir, W.M., G.K. Wong, Y. Zhang, J. Wang and M.A. Groenen et al., 2008. Genome-wide assessment of worldwide chicken SNP genetic diversity indicates significant absence of rare alleles in commercial breeds. Proc. Natl. Acad. Sci. USA., 105: 17312-17317.
    CrossRef    PubMed    

  • Nestor, K.E., Y.M. Saif, J. Zhu and D.O. Noble, 1996. Variation in resistance to Pasteurella multocida among Turkey lines. Poult. Sci., 75: 1161-1163.

  • Redmond, S.B., P. Chuammitri, B.C. Andreasen, D. Palic and J.S. Lamont, 2009. Chicken heterophils from commercially selected and non-selected genetic lines express cytokines differently after in vitro exposure to Salmonella enteritidis. Vet. Immunol. Immunopathol., 132: 129-134.

  • Scanes, C.G., E.A. Dunnington, F.C. Buonomo, D.J. Donoghue and P.B. Siegel, 1989. Plasma concentrations of insulin like growth factors (IGF-I and IGF-II) in dwarf and normal chickens of high and low weight selected lines. Growth Dev. Aging, 53: 151-157.
    PubMed    

  • Schneider, K., R. Klaas, B. Kaspers and P. Staeheli, 2001. Chicken interleukin-6. cDNA structure and biological properties. Eur. J. Biochem., 268: 4200-4206.
    PubMed    

  • Shook, G.E., 1989. Selection for disease resistance. J. Dairy Sci., 72: 1349-1362.
    CrossRef    Direct Link    

  • Swaggerty, C.L., M.H. Kogut, P.J. Ferro, L. Rothwell, I.Y. Pevzner and P. Kaiser, 2004. Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and-susceptible chickens. Immunology, 113: 139-148.
    PubMed    

    • © Science Alert. All Rights Reserved