Biodegradation of Palm Kernel Cake with Multienzyme Complexes from Fungi and its Feeding Value for Broilers
Abstract:
Palm Kernel Cake (PKC) was used as a substrate to elicit the production of polysaccharidases from Aspergillus niger, Trichoderma viride, Rhizopus stolonifer and Mucor mucedo. The extracted enzymes produced were purified and used to ferment PKC in solid state at the rate of 250 ml/kg of the material for 7 days. Unbiodegraded and enzyme degraded PKC were used to formulate broiler starter and finisher diets at the rates of 70 g kg-1 and 100 g kg-1, respectively. A 6th diet was formulated in which Roxazyme G2G, a commercial enzyme was used to supplement the unbiodegraded PKC at the recommended inclusion level of 0.15 g kg-1. A total of 360 1-d-old broiler chicks were randomly allocated to the 6 treatments of 6 replicates each with each replicates having 10 birds. Cellulose and hemicellulose were significantly (p<0.05) reduced in the biodegraded PKC compared with the unbiodegraded PKC and PKC supplemented with Roxazyme G2G. The level of soluble sugars increased in a similar trend. Crude protein, phosphorus and energy increased significantly (p<0.05) in the biodegraded PKC compared to that treated with Roxazyme G2G and the unbiodegraded PKC. Apparent digestibility of nutrients was significantly improved (p<0.05) in birds that received the diets based on the biodegraded PKC than those on the unbiodegraded PKC and Roxazyme G2G supplemented diets. Feed conversion and weight gain in birds were significantly (p<0.05) higher in birds on the diets based on the biodegraded PKC compared to those on diets based on the unbiodegraded PKC and Roxazyme supplemented diets. Results of the study showed that PKC can act as a substrate for the production of a multienzyme complex from the 4 fungi. The enzyme complexes so produced were more efficacious in breaking down the cellulose and hemicellulose in it compared to Roxazyme G2G which is an enzyme product specific for cereal-based diets.
How to cite this article
T. E. Lawal, E. A. Iyayi, B. A. Adeniyi and O. A. Adaramoye, 2010. Biodegradation of Palm Kernel Cake with Multienzyme Complexes from Fungi and its Feeding Value for Broilers. International Journal of Poultry Science, 9: 695-701.
REFERENCES
AOAC., 1995. Official Methods of Analysis of Association of Analytical Chemist International. 16th Edn., AOAC, Washington DC., Pages: 1094
Bachtar, M., 2005. Improvement of nutritive of crop by products using bioprocess technique and their uses for animals. J. Feed Technol., 21: 1-5.
Balagopalan, C. and K.F. Gregory, 1985. Fermentation of cassava starch for single cell protein production. Proceedings of the Seminar on Post Harvest Technology of Cassava, Trivandrum, Kerala, India.
Bedford, M., H.L. Classen and G.L. Campbell, 1991. The effect of pelleting, salt, and pentosanase on the viscosity of intestinal contents and the performance of broilers fed rye. Poult. Sci., 70: 1571-1577.
Direct Link
Classen, H.L., 1996. Cereal grain starch and exogenous enzymes in poultry diets. Anim. Feed Technol., 62: 21-27.
CrossRef Direct Link
Danicke, S., S. Simon, H. Jeroch and M.R. Bedford, 1995. Effect of fat and xylanase supplementation on the intestinal viscosity of rye-fed birds. Proceedings of the 2nd European Symposium on Feed Enzymes, Oct. 25-27, Netherlands, TNO Nutrition and Food research Institute, Zeist Netherlands, pp: 102-107.
Dusterhoff, E.M., 1993. Characteristics and enzymic degradation of non-starch polysaccharides in lignocellulosic by-products: A study on sunflower meal and palm kernel meal. Ph.D. Thesis, Wageningen University, Wageningen.
Felt-Well, R. and S. Fox, 1978. Fuel supplies in the concept of energy. J. Feed Technol., 10: 15-24.
Han, Y.W. and A.W. Anderson, 1975. Semi-solid fermentation of ryegrass straw. Applied Microbial., 30: 930-934.
Direct Link
Hamilyn, P.F., 1998. Fungal Biotechnology. British Mycological Society Newsletter, UK., pp: 130-134
Hesselman, K. and P. Aman, 1986. Inhibition absorption by dietary fibre. J. Anim. Feed Technol., 15: 83-93.
Iyayi, E.A. and Z.A. Aderolu, 2004. Enhancement of the feeding value of some agro-industrial by-products for laying hens after their solid state fermentation with Trichoderma viride. Afr. J. Biotechnol., 3: 182-185.
CrossRef Direct Link
Iyayi, E.A. and D.M. Losel, 2000. Changes in carbohydrates fractions of cassava peel following fungal solid state fermentation. J. Food Technol. Afr., 6: 101-103.
Direct Link
Mikani, Y., K.F. Gregory, W.L. Levadoux, C. Balagopalan and S.T. Whitewill, 1982. Factors affecting yield and safety of protein production from cassava by Cephalosporium eichhorniae. Applied Environ. Microbiol., 43: 403-411.
Direct Link
Manilal, V.B., C.S. Narayanan and C. Balagopalan, 1985. Amyloglucisidae and cellulose activity of Aspergillus niger in cassava starch factory wastes. Proceedings of the Natural Symposium on Production and Utilization of Tropical Crops, November 27-28, 1985, Trivandrum, pp: 211-213.
Naveed, R.F., R. Durrani and M. Farooq, 1999. Broiler production status in district Chakwal. Unpublished report. Poultry Science Department NWFP, Agricultural University Peshwar, Pakistan.
Onilude, A.A. and B.A. Oso, 1999. Effect of fungi enzyme mixture supplementation of various dietary fibres contains diets fed broiler chicks 1 performance and carcass characteristic. World J. Microbiol. Biotechnol., 15: 309-314.
CrossRef
Ofuya, C.O. and C.J. Nwanjuiba, 1990. Microbial degradation and utilization of cassava peel. World J. Microbiol. Biotechnol., 6: 144-148.
CrossRef Direct Link
Pillai, P.B., T. O'Connor-Dennie, C.M. Owens and J.L. Emmert, 2006. Efficacy of an Escherichia coli phytase in broilers fed adequate or reduced phosphorus diets and its effect on carcass characteristics. Poult. Sci., 85: 1737-1745.
Direct Link
SAS, 1999. SAS/STAT. SAS Institute Inc., Cary, N.C.
Smith, J.P., A.J. Rinzema, H.M. Tramper, Van Sonsbeck and W. Krol, 1996. Fibre degradation and microbiology of anabolic zoosponiz fungi critical. Rev. Microbiol., 19: 99-115.
Somogyi, M., 1945. A new reagent for the determination of sugars. J. Biol. Chem., 160: 61-68.
Direct Link
Steel, S.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics. McGraw Hill Book Co., New York, pp: 289
Steenfeldt, S., A. Mullertz and J. Jensen, 1988. Enzyme supplementation of wheat based diets for broilers (1). Effect on growth performance, intestinal viscosity AME and nutrient digestibility. Anim. Feed Sci. Technol., 75: 27-64.
Van Soest, P.J. and M.C. Queen, 1995. The chemistry and estimation of fibre. Proc. Nutr. Soc., 32: 123-130.
Direct Link
Liu, Y. and S.K. Baidoo, 1997. Exogenous enzymes for pig diets: An overview. http://www.idrc.ca/en/ev-30967-201-1-DO_TOPIC.html.
Brana, D.V., M. Ellis, E.O. Castaneda, J.S. Sands and D.H. Baker, 2006. Effect of a novel phytase on growth performance, bone ash and mineral digestibility in nursery and grower-finisher pigs. J. Anim. Sci., 84: 1839-1849.
CrossRef Direct Link
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