Abstract: The present study was conducted to evaluate the haematological and serum biochemical parameters of Nigerian indigenous chickens. Twenty five healthy cocks were randomly assigned to five treatments consisting of intramuscularly administered 6.0, 12.0, 18.0, 12.0 I.U of Pergonal® and sterile water (control). At the end of the three week experimental period, five birds from each group were bled weekly from the wing veins for haematology and serum biochemistry. Results of this study showed no significant differences (P > 0.05) in haematological parameters between the treatments. Serum total protein, albumin, urea and electrolytes differed significantly (P < 0.05) between the treatments. However, the values were within normal range, indicating that Pergonal® had no deleterious effect on these parameters.