Abstract: In this study the effects of Persica and Padina mouthrinses on plaque accumulation and gingivitis were compared with chlorhexidine gluconate 0.2%. This study was a single blind randomized clinical trial. Forty patients with chronic gingivitis participated in this study and were divided into four groups. Gingival index, gingival bleeding index and plaque index were recorded at baseline and after 1, 2, 3 and 4 weeks. Subjects of each group were asked to rinse one of the coded mouth rinses (Chlorhexidine, Persica, Padina and Placebo) twice a day for 4 weeks. During the first two weeks the patients had their habitual oral hygiene but after 2 weeks, oral hygiene was instructed and teeth were scaled and planed. Adjunctive use of Persica and chlorhexidine had only a modest advantage over placebo or Padina. This held true for both plaque (p=0.296) and gingival inflammation indices (p=0.007). However, when these mouthwashes were used as the only oral hygiene procedures and in the presence of calculus, none of them proved to be clinically effective regardless of statistical difference observed between groups (p=0.176 and p=0.001 for plaque and gingival bleeding index, respectively). Use of mouthwashes of any kind alone could not be regarded as substitute for mechanical tooth brushing. In addition adjunctive use of chlorhexidine or Persica proved to have some benefits over placebo and Padina mouthwashes.