Abstract: The aims of this study are to deal with the secondary structures and thermal properties of the Silk Fibroin (SF) films after treatment with different organic solvents; ethanol, ethyl acetate and methanol at various times. Influence of the organic solvents on secondary structure and thermal behavior of the SF films were investigated using Fourier transform infrared (FT-IR) spectroscopy. The results showed the secondary structures of SF films were changed from random coil into high strength, β-sheet structure. With TG, DTG and Heat flow results, the SF films increased their thermal stability after treatment with the organic solvents. However, the stability of SF films did not affect by treatment times. Therefore, a treatment SF film with organic solvent for 30 min is suitable condition. Comparison among organic solvents used, methanol is the best solvent to induce strength and thermal properties of SF films. This study indicated that various organic solvent could be used to improve SF properties with short time treatment.
INTRODUCTION
In last decade, natural polymers have been discovered and were used as bioresources in both technological and biomedical applications (Kweon et al., 2001). Various excellent properties of natural polymers such as non-toxicity, biodegradability and biocompatibility were attracted to study. Therefore, many kinds of natural polymers were reported. However, some limitations such as their high cost and questionable purity have occurred (Cheung et al., 2008). Moreover, natural homopolymer demands are not sufficient for biomaterial products; therefore, blend polymers have been studied to improve the performance of the individual natural polymer (Fan et al., 2008).
Silk is a fibrous polymer that is produced by some Lepidoptera larvae including Bombyx mori silkworm (Altman et al., 2003). Each silk fiber consists of two types of proteins; fibroin and sericin. Silk Fibroin (SF) is an insoluble fibrous component, whereas sericin is a glue-like protein that is well soluble in hot water or some organic solvents. The SF is one of the candidate materials for biomedical applications. Recently, SF has been applied in various fields including cosmetics, medical materials and food additives (Min et al., 2004).
Many factors such as environmental temperature and humidity at spinning, food and healthy condition of silkworm are often significant affects on the SF properties (Zhao et al., 2007). SF films often require chemical treatments in order to enhance their stability and mechanical properties (Tasukada et al., 1995). Methanol is one of frequently used to improve that stability and mechanical properties. However, treatment of SF with methanol can induce high β-sheet structure ratios, resulting of brittle materials and also occur instantly in an uncontrollable crystallization (Jeong et al., 2006). In addition, other solvents and reagents have also used for improving the properties of the films including KCl, NaCl (Vepari and Kaplan, 2007), hexane (Valluzzi et al., 1999), heat (Freddi et al., 1997). Here, we report the effect of different solvents on SF films after submersion in different time points. All SF films were then investigated their secondary structures and thermal behavior. We also attempt to compare present results in order to find the most suitable solvent for enhancing SF film properties.
MATERIALS AND METHODS
This study was constructed for 3 months from May 1, 2009 to August 1, 2009. The experiments were carried out on Department of Chemistry, Faculty of Science. The characterization of secondary structure was done at the Central Instrument, Faculty of Science, Mahasarakham University, Thailand.
Materials
The Bombyx mori silk cocoons were kindly supplied from Silk Innovation
Centre (SIC) Mahasarakham University, Thailand. All used chemical reagents were
analytical grade.
Sample Preparation
The silk cocoons were boiled twice with 1.5% (w/v) Na2CO3
to obtain silk fibroin (SF). The SF was dissolved with tertiary solvent system
of CaCl2:ethanol:H2O (1:2:8 by mol). The solution was
firstly warmed to about 90 °C, then gradually added SF into the solution
and stirred with controlled temperature at 95-100°C until silk already dissolved
(approximately 60 min). The SF hydrolysate was filtrated and then dialyzed in
cellulose tube against distilled water for 3 days at room temperature. The final
concentration after dialysis was adjusted to 1% (w/v) with distilled water.
Silk Fibroin Films Preparation
Ten milliliter of 1% SF solution was cast on 5 cm diameter of polystyrene
plates. The plates were left at 40°C in oven for 3 days. The SF films with
approximately 10-15 μm thickness were obtained.
Organic Solvents Submersion
The SF films were immersed in different kinds of organic solvents: 80% ethanol,
80% ethyl acetate and 80% methanol. The treatment times were assigned for 30,
60 and 90 min. After finish in each time point, the SF films were left in air-dried
and transferred into vacuum oven to make sure the films were completely dried.
Secondary Structure Analysis
The native SF, CS and SF/CS blend films were analyzed for their secondary
structure using FT-IR spectrometer (Perkin Elmer-Spectrum Gx, USA) in the spectral
region of ~2000-400 cm-1 at 4 cm-1 spectral resolution
and 32 scans.
Thermal Behavior Analysis
Eight to ten milligrams were prepared and loaded in a platinum crucible.
The Thermogravimetric Analysis (TGA) was then performed using TA instruments,
SDT Q600 (Lukens drive, New Castle, DE). The samples were non-isothermal
heated from 50 to 1000°C at a heating rate of 10°C min-1.
The TGA was carried out in nitrogen with the flow rate of 100 mL min-1.
The TG and heat flow were recorded with TA instruments Q series explorer
software. The analysis of the data were done using TA Instruments Universal
Analysis 2000 software (version 3.3B).
RESULTS
FT-IR Investigation
The SF films treated with 80% ethanol showed strong absorption bands at
1,635 cm-1 (amide I), 1,539 cm-1 (amide II) and 1,242
cm-1 (amide III) in all of time points (Fig. 1)
while SF films treated with ethyl acetate showed strong absorption bands at
1,630 cm-1 (amide I), 1,525 cm-1 (amide II) and 1,230
cm-1 (amide III) in all of time points (Fig. 2).
On the other hand, SF films treated with methanol showed strong absorption bands
at 1,630 cm-1 (amide I), 1,523 cm-1 (amide II) and 1,240
cm-1 (amide III) in all of time points (Fig. 3).
Thermal Behavior Analysis
Thermogravimetric (TG) curves showed that all of SF films did not completely
decompose even at 1000°C. TG curves of SF films did not much different when
treated with ethanol even increasing of time treatment (Fig. 4).
This result was similar as gave from ethyl acetate (Fig. 5)
and methanol (Fig. 6). The detail of those decomposition peaks
were clearly evidenced by differential thermogravimetric (DTG) curves. For ethanol
treated SF films, the maximum decomposition temperature were 302°C (30 min)
and 305°C (60 and 90 min) (Fig. 7).
Fig. 1: | FT-IR spectra of SF films treated with ethanol at different time point |
Fig. 2: | FT-IR spectra of SF films treated with ethyl acetate at different time point |
Fig. 3: | FT-IR spectra of SF films treated with methanol at different time point |
Fig. 4: | TG curves of SF films treated with ethanol at different time point |
Fig. 5: | TG curves of SF films treated with ethyl acetate at different time point |
Ethyl acetate treated SF films showed maximum decomposition temperature at 302°C with minor peaks at 305°C in all of time set (Fig. 8). In case of SF films treated methanol, maximum decomposition temperature was approximately 306°C (Fig. 9).
Heat Flow Curves
With heat flow curves, all SF films showed thermal stability until above
200°C. Some different endothermic and exothermic temperatures after treatment
SF films with various solvents were observed which were summarized in Table
1. The SF films showed both maximum exothermic and endothermic peaks in
all of organic solvents at 60 min. For ethanol, the maximum of exothermic and
endothermic peaks occurred at 450°C (Fig. 10), while
ethyl acetate was 462°C (Fig. 11). The SF films treated
with methanol showed the most stability compared to other solvents at 463 and
471°C of exothermic and endothermic absorptions, respectively (Fig.
12).
Fig. 6: | TG curves of SF films treated with methanol at different time point |
Fig. 7: | DTG curves of SF films treated with ethanol at different time point |
Fig. 8: | DTG curves of SF films treated with ethyl acetate at different time point |
Fig. 9: | DTG curves of SF films treated with methanol at different time point |
Fig. 10: | Heat flow curves of SF films treated with ethanol at different time point |
Fig. 11: | Heat flow curves of SF films treated with ethyl acetate at different time point |
Fig. 12: | Heat flow curves of SF films treated with methanol at different time point |
Table 1: | Exothermic and endothermic temperature of the SF films treated with different organic solvents |
DISCUSSION
Secondary Structure
The conformational structure of SF has often been studied by FT-IR spectroscopy
(Tasukada et al., 1995) as the IR spectrum indicates
typical absorption bands sensitive to the molecular conformation of SF (Kweon
et al., 2000). The secondary structures of protein are indicated
by the amide I (1700-1600 cm-1), amide II (1600-1500 cm-1)
and amide III (1300-1200 cm-1) bands (Kweon et
al., 2000; Hino et al., 2003). The FT-IR
spectrum of SF films treated with organic solvents showed absorption bands shifted
into lower wave number, indicating the β-sheet structure (Kweon
et al., 2000). The FT-IR results also found that treatment times
did not much affect on the SF film structures. These results suggested that
30 min is enough for organic solvent treatment. The strength of the SF films
after submersion in organic solvents was improved by inducing structural interaction
from random coil to β-sheet structure (She et al.,
2008). The effect of used organic solvents on SF film structures were implied
as methanol>ethyl acetate>ethanol, respectively.
Thermal Behavior Analysis
With TG curves, the initial weight loss at below 100°C was due to the
moisture evaporation from the films (Kweon et al.,
2001). At low temperature, weight of the SF film was gradually loss but
was rapidly loss upper 200°C which associated with the breakdown of side
chain groups of amino acid residues as well as the decomposition of peptide
bonds (Freddi et al., 1997). This finding indicated
that SF has high thermal stability. The thermal decomposition of all SF films
was sharply showed at around 300-305°C. The Td, max of the SF
films treated with organic solvents were sequentially arranged of methanol>ethanol=ethyl
acetate. This result suggested that methanol be most suitable solvent for improving
SF films stability. With heat flow curves, the first endothermic peaks at below
100°C distributions to the dehydration appeared in all of samples. The SF
films treated with methanol appeared sharp endothermic peaks similar patterns
at around 300°C for every treatment times as well as SF films treated with
ethyl acetate. However, the endothermic peaks were less sharp. The SF films
treated with ethanol showed lower of endothermic peaks than other at about 290°C.
The sharp endothermic and exothermic peaks at around 290-300°C have been
described as the strong molecular motion of α-helix and its transformation
into β-sheet structure (Li et al., 2003).
Moreover, all of SF films showed 2 minor endothermic peaks after treatment with
ethanol (327, 450°C), ethyl acetate (340, 460°C) and methanol (340,
475°C), respectively. It is well known that these endothermic are due to
the decomposition of fibroin molecules with unoriented β-sheet structure
(Kweon et al., 2000). Therefore, it is no doubt
that the organic solvents used in this study could be helped to increase thermal
stability of the SF films by changing the higher ratio of secondary structure
from random coil and α-helix into β-sheet structure.
CONCLUSION
The SF films were prepared and used as substrate to treat with organic solvents; ethanol, ethyl acetate. The effects of the solvents and treatment times on morphology, secondary structure and thermal properties of the film were investigated. FT-IR results showed the intermolecular interactions between SF molecules which caused from water dehydration by organic solvents treatments. These results influenced to the secondary structures of SF films which were changed from less strength into high strength, β-sheet structure. With TG, DTG and Heat flow results, the SF films increased their thermal stability after treatment with the organic solvents. However, the stability of SF films did not affect by treatment time. Therefore, treatment SF films with organic solvent for 30 min are enough. Comparison between organic solvents used, methanol is the best solvent to enhance strength and thermal properties of SF films. In conclusion, this study indicated that various organic solvent could be used to improve SF properties with short time treatment. This might be advantage to modify the SF properties for various applications such as biomedicine and pharmaceutics materials.
ACKNOWLEDGMENT
We great fully thank Division of Support and Development, Mahasarakham University and Center of Excellence for Innovation in Chemistry, Commission on Higher Education, Ministry of Education, Thailand for financial support grant of this study.