Viability of Probiotics in Balancing Intestinal Flora and Effecting Histological Changes of Crop and Caecal Tissues of Broilers
Abstract:
The present research was conducted on Hubbard Isa Starbro broilers to evaluate the dynamics of probiotics relating to balanced growth of intestinal flora and histological reactions and changes within the crop and cecal tissues. A total of eighty day-old broiler chicks were divided into four groups of equal numbers as group A (Probiotics fed group vaccinated), B (Probiotics fed group nonvaccinated), C (Conventional fed group vaccinated) and D (Conventional fed group nonvaccinated). Group C and D were taken as control birds fed with commercial ration and groups A and B as experimental birds were fed with commercial ration with the addition of 2 g probiotics (Protexin® Boost)/10 L drinking water upto 6th week of age. The effect of probiotics with regard to clearing bacterial infections and regulating intestinal flora was evaluated by determining the TVC and TLC of the crop and cecum samples of probiotics and conventional fed groups at the 2nd, 4th and 6th week of age. The result revealed competitive antagonism. The result of the study evidenced that probiotic organisms inhibited some nonbeneficial pathogens by occupying intestinal wall space. It has been demonstrated that broilers fed with probiotics had a tendency to display pronounced intestinal histological changes such as active impetus in cell mitosis and increased nuclear size of cells, than the controls. It is obviously found from this research work that probiotics supplementation promoted significant influence on intestinal microbial balance related with pronounced intestinal histological changes.
How to cite this article
S.M.L. Kabir, M.M. Rahman, M.B. Rahman, M.Z. Hosain, M.S.I. Akand and S.K. Das, 2005. Viability of Probiotics in Balancing Intestinal Flora and Effecting Histological Changes of Crop and Caecal Tissues of Broilers. Biotechnology, 4: 325-330.
REFERENCES
Fuller, R., 1989. Probiotics in man and animals. J. Applied Bacteriol., 66: 365-378.
CrossRef PubMed Direct Link
Freter, R., 1956. Experimental enteric shigella and vibrio infection in mice and guinea pigs. J. Exp. Med., 104: 411-418.
Dubos, R.J., 1963. Staphylococci and infection immunity. Am. J. Dis. Child., 105: 643-645.
Ducluzea, R., M. Bellier and P. Raibaud, 1970. Transit digestif de divers inoculums bacteriens introduits Per os chez des souris axeniques et holoxeniques (conventionelles): Effet antagoniste de la microflore du tractus gastro-intestinal. Zentralblatt fur Bakterologie, Parasitenkunde, Infectionskrankheiten und Hygiene, Abt. 1. Orig., 213: 533-548.
Waaij, van der D., J.M.B.D. Vries and J.E.C.L. van der Wees, 1971. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice. J. Hygiene, 69: 405-411.
Lloyd, A.B., R.B. Cumming and R.D. Kent, 1977. Prevention of Salmonella typhimurium infection in poultry by pretreatment of chickens and poults with intestinal extracts. Aust. Vet. J., 53: 82-87.
Fuller, R., 1973. Ecological studies on the lactobacillus flora associated with the crop epithelium of the fowl. J. Applied Bacteriol., 36: 131-139.
Fuller, R. and A. Turvey, 1971. Bacteria associated with the intestinal Wall of the Fowl (Gallus domesticus). J. Applied Bacteriol., 34: 617-622.
Singh, S., V.P. Sharma, V.S. Panwar and S. Singh, 1999. Effect of different levels of probiotic and energy on microbial population in broiler chicks. Ind. Vet. J., 76: 1026-1028.
Shoeib, H.K. and A.H. Madian, 2002. A study on the effect of feeding diets containing probiotics (Pronifer and biogen) on growth performance, intestinal flora and haematological picture of broiler chicks. Assiut. Vet. Med. J., 47: 112-125.
Canalli, L.S., J.S. Flemming, R.T. Mira and L.F. Basile, 1996. Changes in the intestinal microflora of broiler chickens given a probiotic in feeds. Revista Sector Ciencias Agraria, 15: 125-132.
Mahajan, P., K. Suresh, J. Sahoo and S. Kumar, 1999. Effect of Lacto-Sacc (probiotic) feeding on growth promotion and E. coli infection in broilers. Ind. J. Poul. Sci., 34: 99-102.
Li, G., L. Zhou, L. Shao, Q. Li, M. Li, G.P. Li, L.J. Zhou, L.P. Shao, Q.G. Li and M.C. Li, 1999. Effects of feeding diets containing complex live bacterial preparation on E. coli count in caeca, gastro intestinal pH and blood SOD and GSH-PX in chickens. Chinese J. Vet. Med., 25: 7-8.
Endo, T. and M. Nakano, 1999. Influence of a probiotic on productivity, meat components, lipid metabolism, caecal flora and metabolites and raising environment in broiler production. Anim. Sci. J., 70: 207-218.
CrossRef Direct Link
Tarakanov, B., A. Solovev, T. Nikolicheva and T. Bobrova, 1999. A new probiotic. Ptitsevodstvo, 6: 32-33.
Soerjadi, A.S., S.M. Stehman, G.H. Snoeyenbos, O.M. Weinack and C.F. Smyser, 1981. The influence of Lactobacilli on the competitive exclusion of paratyphoid Salmonellae in chickens. Avian Dis., 25: 1027-1033.
Patidar, S.K. and J.B. Prajapati, 1999. Effect of feeding lactobacilli on serum antibody titer and faecal microflora in chicks. Microbiol. Aliments Nut., 17: 145-154.
Samanya, M. and K.E. Yamauchi, 2002. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comparat. Biochem. Physiol. A: Mol. Integrat. Physiol., 133: 95-104.
CrossRef Direct Link
Cowan, S.T., 1985. Cowan and Steel's Manual for Identification of Bacteria. 2nd Edn., Cambridge University Press, Cambridge, London
Gridley, M.F., 1960. Manual of Histologic and Special Staining Technique. 2nd Edn., McGraw Hill Book Co. Inc., New York, pp: 3, 85-86, 132-133
Freed, R.D., 1992. MSTAT Director. Michigan State University, USA
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