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Biotechnology

Year: 2004 | Volume: 3 | Issue: 2 | Page No.: 165-172
DOI: 10.3923/biotech.2004.165.172
Comparison of an Automated Ribotyping System, Pulsed-field Gel Electrophoresis and Randomly Amplified Polymorphic DNA Fingerprinting for Differentiation of Streptococcus uberis Strains
B.E. Gillespie and S.P. Oliver

Abstract: The discriminatory power of three molecular typing methods, automated Ribo printer®, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA fingerprinting (RAPD), was compared by subtyping 46 Streptococcus uberis isolates. Forty-two Streptococcus uberis isolates were cultured from milk samples of dairy cows from various geographical regions and four isolates were reference strains. The automated Ribo printer® identified 29 different strain patterns of S. uberis using the restriction enzyme Pvu II. Thirty-five strain patterns of S. uberis were distinguished with PFGE following Sma I digestion. The RAPD fingerprinting technique distinguished 14 different S. uberis strain patterns. Streptococcus uberis isolates (K577 and K717, M413 and M419 and M526 and M671) were indistinguishable by all three methods of fingerprinting. The PFGE method detected more S. uberis strain pattern differences than the other two methods.

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How to cite this article
B.E. Gillespie and S.P. Oliver, 2004. Comparison of an Automated Ribotyping System, Pulsed-field Gel Electrophoresis and Randomly Amplified Polymorphic DNA Fingerprinting for Differentiation of Streptococcus uberis Strains. Biotechnology, 3: 165-172.

Keywords: ribo printer, RAPD, PFGE, Fingerprinting and Streptococcus uberis

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