Abstract: This study was conducted to determine the replacement of different levels of rapeseed meal with soybean meal as protein source on broilers performance (7 to 49 day old). Experiment design consisted under completely randomized design of a 5 arrangement of dietary treatments with five replicates, a total of 375 (1-day-old) commercial broiler chicks (Ross-308) were randomly distributed into 25 groups consisting of 15 chicks per group. The treatments were (0.0, 4, 8, 12 and 16%) levels of rapeseed meal. The criteria used to assess were weight gain, feed consumption, feed conversion ratio and internal organs weight. Increasing of body weight between treatment in different levels of rapeseed meal and control group showed significant difference (p<0.05). Maximum weight gain belonged to treatment contain 8% and minimum weight gain belonged to treatment contain 16% rapeseed meal. Feed consumption between treatments in different levels of rapeseed meal showed significant difference (p<0.05). However, rapeseed meal had positive effect on feed consumption. Maximum feed consumption belonged to control group and minimum feed consumption belonged to treatment contain 16% rapeseed meal. Feed conversion ratio between treatment in different levels of rapeseed meal showed significant difference (p<0.05). Minimum feed conversion ratio belonged to treatment contain 16% rapeseed meal and maximum feed conversion ratio belonged to control group. There was significant difference between percentage weights of abdominal fat in different levels of rapeseed meal (p<0.05). But there was no significant difference between percentage weights of intestine, liver, pancreas and carcass yield of treatments (p>0.05).
INTRODUCTION
In recent years, there has been an increase in the area of farmlands
allocated for rapeseed in Iran. Moreover, after oil extraction, plenty
of rapeseed meal remains. Therefore, rapeseed can be economically justified
to be used as plant protein substitute for part of soybean meal`s protein.
However, because of some anti-nutrient substances, this meal has to be
limited in the diets. For this reason, there are few researches which
determine the proper level of this meal in the diet for Iranian broilers.
Leeson et al. (1987) has found that rapeseed oil as the only protein
source in diet cause an imbalance between Lysine and Arginine, which is
related to the supplements Lysine, Methionine and Arginine. He also reported
that when Methionine`s availability for both canola and soybean meals
in equally met-based on the chickens feedback to increase growth and feed
consumption-canola meal`s Lysine lick soybean meal`s Lysine will enjoy
a 90% availability. They also concluded that Leucine and Isoleucine inherent
in canola would be limiting for the chicken. Nwokolo and Jeong (1988)
regard that canola as a good source of energy and protein only when used
at 10% level of diet. They have found
that Linoleic acid, significantly accumulated in the muscles, subcutaneous
fat and abdominal cavity of the chickens which had canola meal in their
diet. Oil seed meals are rich in protein and considered as a valuable
animal nutrient. They are obtained as a leftover matter after oil is extracted
from the oil seeds. Oil is extracted from the seeds through either a mechanical
pressure (press) or a solvent, the former of which is more common in Asia
(Karunajewa et al., 1990). Zuprizal and Chagneau (1991) reported
that the true digestibility of canola`s amino acids taken from the peeled
seeds, are 76.9 and 81.4% in broilers respectively. The relative values
in other laboratories are reported 72.8 and 78.3% by the same researchers
respectively. Since sunflower and soybean account for the major share
in the Iranian oil seeds production, failure to expand farming these two
plants as well as the limitation to cultivate them in a few provinces
have made some authorities to think of farming alternative oil plants
specially rapeseed which is cultivated in fall. When importing soybean
meals is indispensable, countries can substitute part of or the whole
meal with other domestic protein sources, leading to economize foreign
exchange. These resources include oilseed meals, grains, green corn powder
and other substances (Modir Sanei, 1993). The rapeseed contains about
42% of oil. Its seed meal has an average of 38% protein, which is widely
used in animal nutrition. The English word Rape, used for such oil plants,
comes from the Latin word Rapum, meaning Turnip. The rape seeds fall commonly
in Brassica, Brassica napus and campestris. These have formed
a meal for humans and cattle since long ago. Nobod knows exactly where
and when they came into being, but it seems that they have originated
in the Mediterranean regions. The ancient scripts show that Rape grew
in India 3000 BC and moved to China and Japan 2000 BC. Historical evidence
represent that Asian and European civilizations used the rapeseed oil
for lightening. It gradually found usage in human nutrition and in producing
industrial oil and soap (Mansouri, 1994). Roth-Maier (2004) with adding
up to 25% level of canola`s whole seed to the diet of broiler chicks concluded
that the added canola to the diet of broiler chicks entails a decrease
in their performance and output. The present research aims to determine
the proper amount of rapeseed meal in the diet for broiler chicks and
to investigate impact of the meal on some of their performance parameters.
MATERIALS AND METHODS
This study was conducted at Shadbad village of Tabriz (Iran) in January
2007. The study included 375 1-day-old (Ross-308) broiler starter. They
received the same diet up to 6 days of age. After the pre-experimental
period, the chicks were weighed and distributed in to 25 groups consisting
of 15 chicks per group. Each floor pen was in to 2x1 m such that the average
weight of all the pens were roughly the same and contained one bell shaped
waterer, feeder and some dry-wood shaving as a litter. Broiler chicks
were fed diet from 7 to 49 days of age. The chicks were maintained in
floor pens with feed and water made available for ad libitum consumption.
The experiment design consisted under completely randomized design of
a 5 arrangement of dietary treatments with five replicates. The treatments
were (0.0, 4, 8, 12 and 16%) levels of rapeseed meal.
The experimental diet were formulated to meet the requirement of broiler
chickens as established by the National Research Council (1994) and based
on energy, protein and nutrients content they were all the same (Table
1-3). Factors of this study are as follows: weight
gain, food consumption, feed conversion ratio and weight of internal organs
(carcass yield, intestine, abdominal fat, liver and pancreas). Body weight
and feed consumption were measured for each pen at were 21, 42 and 49
days of age. Mortality was recorded throughout the experiment and used
to adjust feed consumption data. Two birds per pen were identified by
having body weight closest to the mean body weight of the pen. Then two
birds per pen were killed from these identified birds and their internal
organs weights were measured and considered based on body weight percent.
Table 1: | Ingredients and nutrient composition (%) of experimental diets in starter period |
a: Vitamin supplied kg-1 diet:
vitamin A, 3600000 IU; vitamin D3, 800000 IU; vitamin E,
7200 mg; vitamin K3, 800 mg; pantothenic acid, 4000 mg;
vitamin B1, 1 mg; vitamin B6, 1200 mg; vitamin
biotin, 40 mg; vitamin B2, 2640 mg; nicotinic acid, 12000
mg; vitamin B12, 6 mg; anti-oxidan, 100000 mg b: Mineral supplied kg-1 diet: I, 400 mg; Se, 80 mg; Mn, 40000 mg; Zn, 33800 mg; Fe, 20000 mg; Cu, 4000 mg; Co, 0.1 mg |
Table 2: | Ingredients and nutrient composition (percent) of experimental diets in grower period |
Table 3: | Ingredients and nutrient composition (percent) of experimental diets in finisher period |
General Linear Models (GLM) procedures of SAS7(SAS, 1990) software were employed and significant differences between treatments were separated using Duncan`s new multiple range test.
RESULTS AND DISCUSSION
Analysis of variance of body weight in different rearing periods revealed
that the effect of treatment on body weight during 7-21 days and whole
period was significant (p<0.05) (Table 4). Comparing
means of different treatments of weight gain showed that adding different
levels of rapeseed meal to the diet during 7-21 days and the whole period
significantly improved body weight, as contrasted to the control group
(p<0.05). There was no significant difference among different levels
of rapeseed meal on the body weight. In this study, adding rapeseed meal
at 12 and 16% levels during grower and finisher periods left a numerically
negative effect on broilers` weight, which was not statistically significant
(Table 5). These results were in agreement with those
reported by Blair and Reishert (1984), Fritz et al. (1989), Koreleski
and Rys (1987) and Leeson et al. (1987), but were no consistent
with that reported by Chappa et al. (1989) and Fenwick and Curtis
(1980). It is reported that higher levels of rapeseed meal contain higher
levels of glucosinolate and fiber which lead to a weight loss in broilers
and decrease food consumption (Ajuyah et al., 1991; Kralik
et al., 2003; Roth-Maier, 2004; Thomas et al., 1983). Analysis
of variance of food consumption in different rearing periods revealed
that the effect of treatment on food consumption during 7-21, 22-42 and
43-49 days and the whole period was significant (p<0.05) (Table
6). Comparing means of different treatments of food consumption showed
that with the addition 8, 12 and 16% of rapeseed meal to the diet during
7-21 days, relative to the control group and a treatment contain 4% rapeseed
meal led to decrease in food consumption, which was statistically significant
(p<0.05). There was no significant difference among the treatments
contain 8, 12 and 16% of rapeseed meal on food consumption. Comparing
means of different treatments of food consumption during 22-43, 43-49
days and the whole period indicated that adding different level
Table 4: | Analysis of variance of effects in different dietary levels of rapeseed meal, on Weight Gain (WG), Feed Consumption (FC) and Feed Conversion Ratio (FCR) in broiler chicks` performance from 1 to 7 weeks of age |
NS: Not Significant; *: Significant (p<0.05) |
Table 5: | Comparing means of effects in different dietary levels of rapeseed meal on Weight Gain (WG), Feed Consumption (FC) and Feed Conversion Ratio (FCR) in broiler chicks` performance from 1 to 7 weeks of age |
Values with different superscript letter(s)
are significantly different at p<0.05 |
Table 6: | Analysis of variance of effects in different dietary levels of rapeseed meal, on carcass yield percentage, intestine percentage, abdominal fat percentage, liver percentage and pancreas percentage in broiler chicks` performance from 1 to 7 weeks of age |
ns: not significant; *: Significant (p<0.05) |
Table 7: | Comparing means of effects in different dietary levels of rapeseed meal on carcass yield percentage, intestine percentage, abdominal fat percentage, liver percentage and pancreas percentage in broiler chicks` performance from 1 to 7 weeks of age |
Means in columns with no common superscript differ significantly (p<0.05) |
of rapeseed meal to the diet affects food consumption as a linear decrease
(p<0.05) (Table 7). It seems that decreased feed
intake, by adding the rapeseed meal to the diet, resulting from high glucosinolate
and fiber in this meal. Decreased feed intake resulting from adding levels
of rapeseed meal has been reported by some researches (Slinger et al.,
1978; Summers and Leeson, 1977; Thomas et al., 1983). Analysis
of variance of Feed Conversion Ratio (FCR) in different rearing periods
revealed that the effect of treatment on Feed Conversion Ratio (FCR) during
7-21days and the whole period was significant (p<0.05). Comparing means
of different levels of FCR revealed that adding 8, 12 and 16% rapeseed
meal to the diet during 7-21 days and the whole period, relative to the
control group and the treatment contain 4% rapeseed meal, improved FCR
(p<0.05). There was no significant difference among the treatments
contain 8, 12 and 16% rapeseed meal on FCR. However, during the period
of 7-21 days, the diet of 16% rapeseed meal left an improved value in
FCR, as compared to other treatments. In this study, adding more of rapeseed
meal on the diet led to an improved FCR. A possible explanation for better
FCR could be: (1) adding rapeseed meal resulted in a decrease feed intake,
for this reason, passage time of nutrients from digestive system decrease
and nutrients have enough time for digestion and absorption in digestive
system, (2) In this study, the added rapeseed meals accompanied soybean
oil to balance energy. The soybean oil has some fat which lowers the speed
of food transition within the digestive system and creates a subsequent
chance of more efficient absorption which improves FCR. This is in line
with other researchers` findings (Hulan et al., 1980; Slinger et
al., 1978), while disagreed with the researchers (Blair and Reishert,
1984; Leeson et al., 1987; Reddy et al., 1982; Sadmann and
Schon, 1987).
Analysis of variance of traits in broilers` internal organs weight during
the whole period showed that treatment had an insignificant effect on
the weight of internal organs except abdominal fat (p<0.05). Comparing
means of traits pertaining to the internal organs weight in different
treatments represented that there was significant difference between 8,
12 and 16% levels of rapeseed meal on the abdominal fat weight as contrasted
with the control group treatment and the treatment contain 4% rapeseed
meal (p<0.05). In this respect, a diet of 16% rapeseed meal had the
numerically lowest abdominal fat weight and a diet of 8% rapeseed meal
had the numerically highest abdominal fat weight. In this study the increase
in the amount of rapeseed meal in diet corresponded with a linear decrease
in the abdominal fat weight, which accorded with some researchers (Crespo
and Estve-garsia, 2002; Kralik et al., 2003; Leeson et al.,
1987).
CONCLUSION
The study implies that the treatment contains 8% rapeseed meal can be safely used to feed broilers, without any disturbance on the carcass`s performance and properties. Moreover, such a food cost lower in domestic production inter country, making it a substitute for the soybean meal, which in turn helps decrease of production costs with a subsequent decrease of broilers` total cost.
ACKNOWLEDGMENT
The authors gratefully acknowledge the assistant professors of Shabestar Islamic Azad University, Iran.