Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Review Article
 
Bauhinia purpurea Linn.: A Review of its Ethnobotany, Phytochemical and Pharmacological Profile



T. Kumar and K.S. Chandrashekar
 
ABSTRACT

The use of natural products as medicinal agents presumably predates the earliest recorded history. Bauhinia purpurea is a species of flowering plant is used in several traditional medicine systems to cure various diseases. This plant has been known to possess antibacterial, antidiabetic, analgesic, anti-inflammatory, anti-diarrheal, anticancerous, nephroprotective and thyroid hormone regulating activity. A wide range of chemical compounds including 5,6-Dihydroxy-7-methoxyflavone 6-O- β –D xylopyrano-Side, bis [3’,4’-dihydroxy-6-methoxy-7,8-furano-5’,6’-mono-methylalloxy]-5-C-5-biflavonyl and (4’-hydroxy-7-methyl 3-C-α-L-rhamnopyranosyl)-5-C-5-(4’-hydroxy-7- methyl-3-C-α-D-glucopyranosyl) bioflavonoid, bibenzyls, dibenzooxepins, mixture of phytol fatty esters, lutein, β-sitosterol, isoquercitin and astragalin etc. The present review discusses phyto-chemistry, pharmacology, medicinal properties and biological activity of B. purpurea and its usage in different ailments.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

T. Kumar and K.S. Chandrashekar, 2011. Bauhinia purpurea Linn.: A Review of its Ethnobotany, Phytochemical and Pharmacological Profile. Research Journal of Medicinal Plants, 5: 420-431.

DOI: 10.3923/rjmp.2011.420.431

URL: https://scialert.net/abstract/?doi=rjmp.2011.420.431
 
Received: August 30, 2010; Accepted: September 09, 2010; Published: November 08, 2010

INTRODUCTION

Nature has provided a complete storehouse of remedies to cure ailment of mankind. Medicinal plants have been used for centuries as remedies for disease because they contain component of therapeutic values. According to the WHO, 80% of the world population continues to rely mainly on traditional medicines for their health care (WHO, 1993). Herbal medicines, as the major remedy in traditional medical systems, have been used in medical practice for thousands of years and have made a great contribution to maintaining human health. A majority of the world’s population in developing countries still relies on herbal medicines to meet its health needs. The attention paid by health authorities to the use of herbal medicines has increased considerably, both because they are often the only medicine available in less developed areas and because they are becoming a popular alternative medicine in more developed areas (Gurib-Fakim, 2006). The continued investigation into the secondary plant metabolites has gained importance for their safe use.

GEOGRAPHICAL DISTRIBUTION

Bauhinia purpurea L. is a medium sized deciduous tree belongs to the family Leguminosae (Caesalpinioideae), native to South China (which includes Hong Kong) and Southeastern Asia and it is found throughout India, ascending to an altitude of 1300 m in the Himalayan (Khare, 2004).

B. purpurea is a moderate evergreen tree in sub-Himalayan region and western track of India and often its leaves are used as fodder during the lean period (Jha, 1995). The genus Bauhinia, consisting of 300 species (Chopra et al., 1996). In the United States of America, the tree grows in Hawaii, coastal California, southern Texas and southwest Florida. Common names include Hong Kong Orchid Tree, Purple camel's foot and Hawaiian orchid tree.

Plant profile: It is a small to medium-sized deciduous tree growing up to 17 m tall. The bark is ashy to dark brown, nearly smooth, young parts brown-pubescent. The leaves are 7.5-15 cm long, rather than longer than broad, cleft about half way down into 2 acute or rounded bilobed very minutely pubescent beneath when young, base usually cordarte, 9-11 nerved; petiole 2.25-3.8 cm long. The flowers are conspicuous, pink and fragrant with five petals. Pedicels 5-13 mm long, stout, tementose, bract and bracteoles small tementose, deltoid. Calyx tementose, tube 7.5-10 mm long, limb long as the tube. Petals 3.8 to 5 cm long, oblanceolate, long clawed, spreadind veined. Stamens usually 3 fertile, others reduced to antherless filaments. Ovary downy, long-stalked; style long; stigma large, oblique. Pod 15-25 by 1.5-2 cm on a tementose stipe 1.5 to 2.5 cm long, linear, flat, pointed, greenish, tinged with purple till ripe, late in dehiscing. Seeds 12-15 suborbicular, flattened, 1.3 cm. wide and dark brown smooth (Kritikar and Basu, 1991).

Indian names: In India it is known by its various vernacular names, the most commonly used ones are Orchid tree (English), Khairwal, Kaniar (Hindi), Sarul (Kannada), Chuvanna Mandaram (Malayalam), Vanaraja (Sanskrit), Mandari (Tamil), Bodanta (Telgu), Kaanchanaara, Kaanchana (Ayurveda): Sivappumanchori (Siddha).

Medicinal uses: The young pods and mature seeds of kachnar are known to be cooked and eaten by tribles such as the Kathkors and Gondas of India (Rajaram and Janardhanan, 1991). Species of Bauhinia are rich in polyphenolics and are known for its medicinal properties (Patil, 2003). B. purpurea known to the Malays as pokok tapak kerbau, has been traditionally used by the Indian, Sri Lankan and Pakistani people to treat ailment like ulcer, wound, glandular swelling and stomach tumor. The decoction of the root is used for expelling gases, flatulence and griping pain from the stomach and bowel, the bark of the plant is used as an astringent in the treatment of diarrhea. Its decoctions are recommended for ulcers as a useful wash solution. The bark or root and flower mixture with boiled rice water is used as maturant for boils and abscesses (Kurian, 2004). The decoction of flower works as a laxative (Wassel et al., 1986). Fresh bark of Kaanchanaara (B. purpurea) mixed with Shunthi (dry Zingiber offficinale), pounded with sour gruel, was prescribed in enlarge cervical glands (Vrindamaadhava) as well as in goiter (Shaarangadhara Samhitaa, Bhavaprakaasha). Over the counter Kaanchanaara (B. purpurea) Guggulu (Shaarangadhar Samhitaa) is used to treat enlarge cervical glands, goiter and scrofulous tumors, so is kaanchan-gudikaa (Bhaishjya Ratnaavali). It has also been reported to contain high phenolics which are usually referred to as anti-quality factor for ruminant nutrition’s because of their high affinity with proteins (Yadav and Bhadoria, 2001). Although, there is no documentation on its traditional use to treat diseases among the Malaysians, this plant has been used in the Indian, Sri Lankan and Pakistani folklore medicine to treat ailments like glandular swellings, skin diseases, ulcers, diarrhea, stomach tumors and wounds (Jones and German, 1993). Several ethnomedicinal importance of B. purpurea is given in Table 1.


Table 1:

Ethnomedicinal uses of different parts of B. purpurea Linn.

PHYTOCHEMISTRY OF B. PURPUREA

B. purpurea contain major class of secondary metabolites are glycosides, flavonoids, saponins, triterpenoids, phenolic compounds, oxepins, fatty acids and phytosterols. From the ethanolic extract of the whole plant of B. purpurea two new oxepins named bauhiniastatins1 and 2 have been isolated and the ethanolic extract of root provides bauhiniastatins 1, 2, 3 and pacharin (Fig. 1) exhibit significant growth inhibition against a minipanel of human cancer cell lines (Pettit et al., 2006). The structures have been established on the basis of chemical evidence and spectroscopic methods. A novel flavone glycoside, 5,6-dihydroxy-7-methoxyflavone 6-O-b-D-xylopyranoside (Fig. 2) was isolated from the chloroform-soluble fraction of the ethanolic extract of B. purpurea stems (Yadav and Tripathi, 2000). Three glycerol derivatives and 6- butyl-3-hydroxyflavanone derivatives are 2, 3-dihydroxypropyl oleate, 2,3 dihroxypropyl linoleate, 2,3- dihydroxypropyl 16-hyroxy–decanoate and 6-butyl-3-hydroxyflavanone, 6-(3”-oxobutyl)-taxifolin (Fig. 3) respectively isolated from methanolic extract of heartwood of B. purpurea (Kuo et al., 1998). The two new dimeric flavonoids namely bis [3’,4’-dihydroxy-6-methoxy-7,8-furano-5’,6’-mono methylalloxy]-5-C-5-biflavonyl and (4’-hydroxy-7-methyl 3-C-α-L-rhamnopyranosyl)-5-C-5-(4’-hydroxy-7- methyl-3-C-α-D-glucopyranosyl) bioflavonoid (Fig. 4) with protein precipitating property obtained from 70% aq. acetone extract of B. purpurea leaves (Yadav and Bhadoria, 2005). The leaves of B. purpurea also afforded a mixture of phytol fatty esters, leutin and β-sitosterol (Fig. 5) (Ragasa et al., 2004). The petroleum ether fraction of ethanolic extract (95%) of Bauhinia purpurea leaf gave α- amyrin caprylate on successive column chromatography with petroleum ether (60-80°) and chloroform which gives Liebermann-Burchard test of triterpene. The compound is characterized by spectral analysis (Verma and Chandrashekar, 2009). In the flower volatile oils of both B. purpurea and B. variegata found monoterpenes (e.g., a-terpinene, limonene, myrcene, linalool, citronellyl acetate) and a phenylpropanoid (eugenol) (Wassel et al., 1986). The aqueous methanolic extract of fresh flower of B. purpurea gives flavonoid quercetin and flavonoid glycosides isoquercitin, astragalin (Fig. 6) (Ramchandra and Joshi, 1967) butein 4’ O-β-L-arabinopyranosyl-O-β-D-galactoside (mp 265°) isolated from seed of B. purpurea. This gave the characteristic colour reactions of a chalcone and ion hydrolysis with 8% ethanolic H2SO4 for 12 h gave butein and a disaccharide, the component sugars which were found as galactose and arabinose (Bharatiya et al., 1979).


Fig. 1:

Structure of oxepins


Fig. 2:

Structure of flavone glycoside


Fig. 3:

Structure of 6-(3"-oxobutyl)-taxifolin

A new glycoside 3,4-dihydroxychalcone 4-O-β-L-arabinopyranosyl-O-β-D-galactopyranoside (mp 365°) isolated from seed which gave the characteristic colour reactions of a chalcone and gave 3,4-dihydroxychalcone, galactose and arabinose on acid hydrolysis (8% ethanolic H2SO4 for 12 h). The identity of sugars was confirmed by co-chromatography with authentic samples and by the preparation of their osazones (Bharatiya and Gupta, 1981). After chalcone glycoside a novel flavone glycoside were isolated, Glycoside-6-4’-Diahydroxy-3’-prenyl-3,7,5,7’-Tetramethoxy Flavone-6-O-α-L-rhamnopyranoside (Fig. 7) from acetone soluble of ethanolic extract from seed of B.


Fig. 4:

Structure of dimeric flavonoids


Fig. 5:

Structure of leutin and beta-sitosterol


Fig. 6:

Structure of flavonoids and flavonoid glycosides

purpurea which gives positive test for Molisch and structure are confirmed by spectral data analysis (Yadav and Sodhi, 2001). The CH2Cl2 extract of root of B. purpurea on purification yield 11 new compounds bauhinoxepin C-J, bauhinobenzofurin A, bauhispirorin A, bauhinol E, two flavanones (-)-strobopinin and demethoxymatteucinol and five known bibenzyls (Fig. 8) which posses various pharmacological activities (Boophong et al., 2007). All the compounds were characterized by spectral analysis. Kachnar (B. purpurea) seeds were found to contain about 17.5% crude seed oil. The amount of neutral lipids in the crude seed oil was the highest (99% of total lipids), followed by glycolipids and phospholipids, respectively. Linoleic, followed by palmitic, oleic and stearic, were the major fatty acids in the crude seed oil and its lipid classes. The ratio of unsaturated fatty acids to saturated fatty acid, was higher in neutral lipid classes than in the polar lipid fractions. The oil was characterized by a relatively high amount of phytosterols, wherein the sterol markers were β-sitosterol and stigmasterol. β-Tocopherol was the major tocopherol isomer with the rest being d-tocopherol (Ramadana et al., 2006). Bauhinia purpurea seed is a source of galactose and lactose binding lectin, a peptide which interact with carbohydrate. The amino acid sequence of peptide that bind with lactose is Asp-Thr-Trp-Pro-Asp-Thr-Glu-Trp-Ser and is obtained of Bauhinia purpurea lectin by affinity chromatography of peptide with Asp-N endoproteinase or trysin on column of lactose-Sepharose 4B or lactose-, maltose-, fucose- and di-N-ucetylchitobiose-Sepharose and by solid phase synthesis.


Fig. 7:

Structure of novel flavone glycoside

This peptide exhibits lactose binding activity in the presence of calcium (Yamamoto et al., 1991).

PHARMACOLOGICAL PROPERTIES OF B. PURPUREA

Antinociceptive, anti-inflammatory, analgesic and antipyretic properties: The aqueous extract of leaf of B. purpurea possesses good antinociceptive, anti-Inflammatory, analgesic and antipyretic. The crude dried extract was prepared in doses of 6.0, 30.0 and 60.0 mg kg-1 and subjected to the respective. They have used antinociceptive (abdominal constriction, hot plate and formalin tests), anti-inflammatory (carrageenan-induced paw edema test) and antipyretic (brewer’s yeast-induced pyrexia test) assays. The 6.0 mg kg-1 AEBP exhibited the highest antinociceptive activity, the 30.0 mg kg-1 AEBP exhibited an equieffective anti-inflammatory activity when compared to the 100 mg kg-1 ASA only between the interval times of 1-4 h, The dose-independent antipyretic activity was observed only at the concentration 6.0 and 30.0 with the former showing remarkable activity even when compared with 100 mg kg-1 ASA (Zakaria et al., 2007). In Zakaria et al. (2009) established the antinociceptive and anti-inflammatory activities of chloroform extract of B. purpurea leaves using animal models. The different dose 20, 100, 200 mg kg-1 were prepared in dimethyl solfoxide were 100 mg kg-1 extract showed a less remarkable anti-inflammatory activity compared to the other doses tested. Analgesic and anti-inflammatory activities of ethanolic extract of stem of B. purpurea was subjected. Different CNS depressant paradigms like analgesic activity (Eddy’s hot plate method and acetic acid writhing method) and anti-inflammatory activity (carrageenan induced paw edema) were carried out following the intra peritoneal administration of extract at dose level 50 and 100 mg kg-1. Dose of 100 mg kg-1 was comparable with standard drugs (Shreedhara et al., 2009). The aqueous and methanolic extract of the stem bark of Bauhinia purpurea were tested for anti-inflammatory activity at dose level 300 mg kg-1 by carageenan induced rat paw edema. Both the extract were tested against standard drug diclofenac were ethanolic extract showed maximum activity, However the extracts activity is less than standard drug (Chandrashekar et al., 2009a, b).


Fig. 8: Structure of oxepins, flavones and bibenzyls

The ethyl acetate extract of stem bark of Bauhinia purpurea were found good analgesic activity tested at dose level 400 mg kg-1 by acetic acid induced writhing model and hot plate method (Chandrashekar et al., 2009a).

Antimalarial, antimycobacterial, antifungal and cytotoxicity activities: The isolated compounds from roots exhibited antimycobacterial activity with MIC valve ranging from 24.4 to 740.7 μM. Among all compounds bauhinoxepin J is a potent antimycobacterial agent activity having MIC 24.4 μM. Among the isolated metabolites, compounds 6, 7, 8 and 13 exhibited antimalarial activity (IC50 5.8-11.2 μM), while compounds 1, 4, 9, 15 and 18 exhibited antifungal activity (IC50 49.6-130.1 μM). Compounds 1, 2, 4, 6, 7, 8 and 18 exhibited cytotoxicity towards KB and BC cell line with IC50 values ranging from 10.5 to 72.3 μM. Compound 4 and 7 posses potent anti-inflammatory activity inhibiting the COX-2 enzyme with IC50 value of 6.9 and 10.1 μM respectively (Fig. 8) (Boophong et al., 2007).

Anti-diabetics: The rat showing blood glucose level 250-350 mg dL-1 were considered as diabetic rat, induced by alloxan. The hypoglycemic activity of ethanolic extract and purified fraction-1 of stem of B. purpurea were studied and found that the dose of 100 mg dL-1 (i.p.) reduces serum glucose level of Wister rats due to inhibition of cyclooxygenase and promote β-cell regeneration (Muralikrishna et al., 2008).

Cardiac activity: The cardiotonic activity of purified fraction-1 of ethanolic extract of stem of B. purpurea were studied and found that the fraction-1 has exhibited positive inotropic and chronotropic effect on isolated frog’s heart. Its action is blocked by β2-adrenergic blocker propranolol. The characterization of the isolated compound based on structural studies is under progress (Muralikrishna et al., 2008).

Hormone regulation: The aqueous alcoholic bark extract of B. purpurea (2.5 mg kg-1 b.wt.) and aqueous root extract Withania somnifera (1.4 g kg-1 b.wt.) on daily administration for 20 days, stimulating thyroid function in female mice. Both the plant extracts showed an increase in hepatic glucose-6-phosphatase (G-6-Pase) activity and antiperoxidative effects as indicated either by a decrease in hepatic lipid peroxidation (LPO) and/or by an increase in the activity of antioxidant enzyme(s). Serum triiodothyronine (T3) and thyroxine (T4) concentrations were increased significantly by Bauhinia, Withania could enhance only serum T4 concentration (Panda and Kar, 1999). In Panda et al. (2003) studied the role of Emblica officinalis L. and Bauhinia purpurea L. extracts in regulating thyroid functions was studied in male mice. Oral administration of Emblica officinalis L. fruit extract at 30 mg kg-1 body weight (b.wt.) each day for 20 days decreased serum T3 and T4 concentrations and hepatic O2 consumption. In contrast daily administration of B. purpurea at 2.5 mg kg-1 b.wt. each day for 20 days increased serum T4 concentration and O2 consumption. Both the plant extracts exhibited hepatoprotective effects as evidenced by decreased lipid per oxidation (Panda et al., 2003).

Antioxidant activity: The antioxidant activity of ethanolic extract (95% v/v) of leaves of B. purpurea exhibited significant free radical scavenging activity and reducing power activity when compare with ascorbic acid. The IC50 values were found to be 78.31 and 59.37 μg mL-1 for ethanolic extract of leaves of B. purpurea and ascorbic acid, respectively (Joshi et al., 2009). The ethanolic extracts of aerial parts do not shows antioxidant activity (Silva et al., 2005).

Nephroprotective: The ethanolic extract of leaves and unripe pods of B. purpurea shows protective action on kidney induced by gentamicin induced nephrotoxicity. Extracts were administered intraperitoneal at dose level 300 mg/kg/day for eight days reduces blood vessel congestion, epithelial desquamation, accumulation of anti-inflammatory cells and necrosis of kidney cells. This normalizes the increased level of serum creatinine, uric acid, urea and blood urea nitrogen (Lakshmi et al., 2009).

Wound healing activity: Four different models excision, incision, burn and dead space wound were used to determine wound healing properties of chloroform and methanol extracts of leaves of B. purpurea. Low dose 2.5% (w/w) and high dose 5% (w/w) of chloroform and methanol extracts were prepared in hydrophilic and hydrophobic bases for excision, incision, burn wound models applied topically. Aloe vera 5% (w/w) was used as a standard. For dead space wound model 100 and 500 mg kg-1 and as a standard Aloe vera 300 mg kg-1 were given orally. B. purpurea is having almost equal activity with Aloe vera in all four wound healing models (Ananth et al., 2010).

Anti-diarrheal activity: The ethanolic extract of leaves shows inhibitory effect at different dose level on animal models castor oil induced diarrhea in rats and gastrointestinal motility test by using charcoal meal. This inhibitory effects support the use of the leaves of B. purpurea in folklore medicine (Mukherjee et al., 1998).

CONCLUSIONS

The scientific research on B. purpurea is suggests a huge biological potential of this plant. It is strongly believed that detailed information as presented in this review on the phytochemical and various biological properties of the extracts might provide detailed evidence for the use of this plant in different medicines. The phytochemical variations and efficacy of the medicinal values of B. purpurea is dependent on geographical locations.

Even today, plants are the almost exclusive source of drugs for a majority of the world population. Therefore, it remains a challenge for scientist to provide efficient, safe and cheap medication especially for rural area. These Bauhinia species and their quantification of individual phytoconstituents as well as pharmacological profile based on in vitro, in vivo studies and on clinical trial should be further investigated.

REFERENCES
Ananth, K.V., M. Asad, N.P. Kumar, S.M.B. Asdaq and G.S. Rao, 2010. Evaluation of wound healing potential of Bauhinia purpurea leaf extracts in rats. Indian J. Pharmaceut. Sci., 72: 122-127.
CrossRef  |  Direct Link  |  

Asolkar, L.V., K.K. Kakkar and O.J. Chakre, 2000. Second Supplement to Glossary of Indian Medicinal Plants with Active Principles. Part-1 (AK). National Institute of Science Communication, New Delhi, pp: 116-117.

Bhartiya, H.P. and P.C. Gupta, 1981. A chalcone glycoside from the seeds of Bauhinia purpurea. Phytochemistry, 20: 2051-2051.
CrossRef  |  

Bhartiya, H.P., P. Dubey, S.B. Katiyar and P.C. Gupta, 1979. A new chalcone glycoside from Bauhinia purpurea. Phytochemistry, 18: 689-689.

Boonphong, S., P. Puangsombat, A. Baramee, C. Mahidol, S. Ruchirawat and P. Kittakoop, 2007. Bioactive compounds from Bauhinia purpurea possessing antimalarial, antimycobacterial, antifungal, anti-inflammatory and cytotoxic activities. J. Nat. Prod., 70: 795-801.
CrossRef  |  PubMed  |  Direct Link  |  

Chandrashekar, K.S., T. Kumar, A.B. Joshi, S. Saha, Hitesh and K.S. Prasanna, 2009. Anti-inflammatory activity of Bauhinia purpurea stem barks extract against carageenan induced rat paw edema. Herbal Heritage, 1: 42-45.

Chandrashekar, K.S., T. Kumar, M.P. Gururaj and H. Joshi, 2009. Analgesic activity of Bauhinia purpurea Linn. Herbal Heritage, 1: 94-96.

Chatterjee, A. and S.C. Pakrashi, 1992. The Treatise of Indian Medicinal Plants. Vol. 2, Council of Scientific and Industrial Research, New Delhi.

Chopra, R.N., S.L. Nayar and I.C. Chopra, 1996. Glossary of Indian Medicinal Plants. CSIR, New Dehli, ISBN: 8172361262.

Das, A.K., B.K. Datta and G.D. Sharma, 2008. Medicinal plants used by different tribes of Cachar district, Assam. Ind. J. Trad. Knowledge, 7: 446-454.
Direct Link  |  

Gurib-Fakim, A., 2006. Medicinal plants: Traditions of yesterday and drugs of tomorrow. Mol. Aspects Med., 27: 1-93.
CrossRef  |  PubMed  |  Direct Link  |  

Jha, L.K., 1995. Advances in Agroforestry. APH Publishing Corporations, New Delhi, India, pp: 669.

Jones, D.T. and P. German, 1993. Flora of Malaysia. Oxford University Press, Oxford, pp: 154.

Joshi, V.D., T. Verma and P.R. Shetty, 2009. Antioxidant potential of Bauhinia purpurea Linn. leaves. Int. J. Pharm. Res., 1: 51-55.
Direct Link  |  

Kalakoti, B.S. and Y.P.S. Pangtey, 1988. Ethno-medicine of Bhotia tribes of Kumaon Himalaya, UP. Bull. Med. Ethno. Bot. Res., 9: 11-20.

Kamble, S.Y., S.R. Patil, P.S. Sawant, S. Sawant, S.G. Pawar and E.A. Singh, 2010. Studied on plant used in traditional medicine by Bhilla tribe of Maharashtra. Indian J. Trad. Knowledge, 9: 591-598.
Direct Link  |  

Khare, C.P., 2004. Encyclopaedia of Indian Medicinal Plant. Springer-Verlag, New York, pp: 95-96.

Kritikar, K.R. and B.A. Basu, 1991. Indian Medicinal Plants. 2nd Edn., Periodical Experts Book Agency, New Delhi, India, pp: 856-860.

Kuo, Y.H., M.H. Yeh and S.L. Huan, 1998. A novel 6- butyl-3-hydroxyflavanone from heartwood of Bauhinia purpurea. Phytochemistry, 49: 2529-2530.
CrossRef  |  

Kurian, J.C., 2004. Plant that Heal. 7th Edn., Oriental Watchman Publishing House, Pune, pp: 31.

Lakshmi, B.V.S, N. Neelima, N. Kasthuri, V. Umarani and M. Sudhakar, 2009. Protective Effect of Bauhinia purpurea on gentamicin induced nephrotoxicity in rat. Indian J. Pharm. Sci., 71: 551-554.
CrossRef  |  

Mukherjee, P.K., T.K. Gopal and T. Subburaju, 1998. Studies on the anti-diarrheal profiles on Bauhinia purpurea Linn. leaves extract. Nat. Prod. Sci., 4: 234-237.

Muralikrishna, K.S., K.P. Latha, C.S. Shreedhara, V.P. Vaidya and A.M. Krupanidhi, 2008. Effect of Bauhinia purpurea Linn. on alloxan-induced diabetic rats and isolated frogs heart. Int. J. Green Pharm., 2: 83-86.

Panda, S. and A. Kar, 1999. Withania somnifera and Bauhinia purpurea in the regulation of circulating thyroid hormone concentration in female mice. J. Ethnopharmacol., 67: 233-239.
PubMed  |  

Panda. S., A. Karr and S. Bharti, 2003. Regulation of thyroid function in mice with extract of Emblica officinalis L. and Bauhinia purpurea Linn. J. Herb Spices Med. Plants, 10: 1-9.
CrossRef  |  

Patil, G.G., P.Y. Mali and V.V. Bhadane, 2008. Folk remedies used against respiratory disorders in Jalgaon district, Maharashtra. Nat. Prod. Radiance, 7: 354-358.
Direct Link  |  

Patil, V.K., 2003. Prospect and Potential of Medicinal and Aromatic Plant in Chhattisgarh. IG Agriculture University, Raipur, India, pp: 17.

Pawar, S. and D.A. Patil, 2007. Ethnomedicinal uses of barks in Jalgaon district. Nat. Prod. Radiance, 6: 341-346.
Direct Link  |  

Pettit, G.R., A. Numata, C. Iwamoto, Y. Usami, T. Yamada, H. Ohishi and G.M. Cragg, 2006. Antineoplastic agent 551 isolation and structure of Bauhiniastatins-1-4 from Bauhinia purpurea. J Nat. Prod., 69: 323-327.
PubMed  |  

Ragasa, C.Y., J.G. Hofilena and J. Rideout, 2004. Secondary metabolite from Bauhinia purpurea. J. Sci. Food Agric., 55: 423-431.

Rajaram, N. and K. Janardranan, 1991. Chemical composition and nutritional potential of the tribal pulse, Bauhinia purpurea, B. racemosa and B. vahlii. J. Sci. Food Agric., 55: 423-431.

Ramadana, M.F., G. Sharanabasappab, Y.N. Seetharamb, M. Seshagiric and J.T. Moerseld, 2006. Characterisation of fatty acids and bioactive compounds of kachnar (Bauhinia purpurea L.) seed oil. Food Chem., 98: 359-365.
CrossRef  |  Direct Link  |  

Ramchandra, R. and B.C. Joshi, 1967. Chemical examination of Bauhinia purpurea flower. Curr. Sci., 36: 574-575.
Direct Link  |  

Shiddamallayya, N., A. Yasmeen and K. Gopakumar, 2010. Medico-botanical suvey of Kumar pavatha Kukke Subramanya, Manglore, Karnataka. Indian J. Trad. Knowledge, 9: 96-99.
Direct Link  |  

Shreedhara, C.S., V.P. Vaidya, H.M. Vagdevi, K.P. Latha, K.S. Muralikrishna and A.M. Krupanidhi, 2009. Screening of Bauhinia purpurea Linn. for analgesic and anti-inflammatory activities. Indian J. Pharmacol., 41: 75-79.
CrossRef  |  

Silva, C.G., R.S. Herdeiro, C.J. Mathias, A.D. Panek and C.S. Silveira et al., 2005. Evaluation of antioxidant activity of Brazilian plants. Pharmacol. Res., 52: 229-233.
PubMed  |  

Verma, T., K.S. Chandrashekar and B.J. Arun, 2009. α-Amyrin caprylate: A new triterpene isolated from the leaf of Bauhinia purpurea Linn. Asian J. Res. Chem., 2: 569-570.
Direct Link  |  

WHO, 1993. Research Guideline for Evaluating the Safety and Efficacy of Herbal Medicines. World Health Organization, Manila, Philippines, Pages: 2.

Wassel, M., S.M. Abdel-Wahab and N.M. Ammar, 1986. Constituents of the essential oils from Bauhinia variegata L. and Bauhinia purpurea L. flowers. Sci. Pharm., 54: 357-361.

Yadav, R.N. and P. Tripathi, 2000. A novel flavone glycoside from the stem of Bauhinia purpurea. Fitoterapia, 71: 88-90.
PubMed  |  

Yadav, R.N. and S. Sodhi, 2001. A novel flavone glycoside-6-4-diahydroxy-3-prenyl-3,7,5,7-tetramethoxy flavone-6-O-α-L-rhamnopyranosidewas isolated from seed of Bauhinia purpurea. Asian J. Chem., 13: 529-533.

Yadav, S. and B.K. Bhadoria, 2001. Chemical and biochemical assessment of some leguminous tree leaves. Indian J. Anim. Nutr., 18: 113-118.

Yadav, S. and B.K. Bhadoria, 2005. Two dimeric flavonoids from Bauhinia purpurea. Ind. J. Chem., 44: 2604-2607.
Direct Link  |  

Yamamoto, K., Y. Konami, K. Kusui and T. Osawa, 1991. Purfication and characterization of a carbohydrate-binding peptide from Bauhinia purpurea lectin. FEBS Lett., 281: 258-262.
PubMed  |  

Zakaria, Z.A., N.I.A. Rahman, Y.W. Loo, A.H.A. Ayub and M.R. Sulaiman et al., 2009. Antinociceptive and anti-inflammatory activities of the chloroform extract of Bauhinia purpurea L. (Leguminosae) leaves in animal models. Int. J. Trop. Med., 1: 140-145.

Zakaria, Z.A., Y.W. Loo, N.I. Abdul-Rahman, A.H. Abdul-Ayub, M.R. Sulaiman and G.H. Kumar, 2007. Antinociceptive, anti-inflammatory and antipyretic properties of Bauhinia purpurea leaves aqueous extract in experimental animals. Med. Prin. Prac. 16: 443-449.
Direct Link  |  

©  2018 Science Alert. All Rights Reserved
Fulltext PDF References Abstract