The Growing Importance of Neem (Azadirachta indica A. Juss) in Agriculture, Industry, Medicine and Environment: A Review
There has been astronomical increase in the costs of chemical fertilizers, agrochemicals, animal feeds and synthetic drugs in the developing countries, with an increasing indebtedness and acute poverty. This situation exerts enormous pressure to explore local resources, handy to combat these deficits and improve quality of life of the people. One of such available resources with great potentials in the 21st century is the neem (Azadirachta indica A. Juss) tree. Neem belongs to the family Meliaceae. It is the most versatile, multifarious trees of tropics, with immense potentials. It possesses maximum useful non-wood products such as leaves, bark, flowers, fruits, seeds, gum, oil and neem cake than any other tree species. Biologically neem has numerous bioactive ingredients with diverse applications. These bioactive ingredients are known to have antiallergenic, antidermatic, antifeedent, antiviral, antifungal, anti-inflammatory, antipyorrhoeic, antiscabic, insecticidal, larvicidal, anti-implantation, nematicidal, spermatocidal and other biological activities. This review is an attempt to assemble all the major research findings in neem which is of direct relevance to environment, industry, medicine and agriculture.
to cite this article:
I.P. Ogbuewu, V.U. Odoemenam, H.O. Obikaonu, M.N. Opara, O.O. Emenalom, M.C. Uchegbu, I.C. Okoli, B.O. Esonu and M.U. Iloeje, 2011. The Growing Importance of Neem (Azadirachta indica A. Juss) in Agriculture, Industry, Medicine and Environment: A Review. Research Journal of Medicinal Plants, 5: 230-245.
Received: March 21, 2010;
Accepted: May 06, 2010;
Published: August 13, 2010
The neem is a tropical evergreen tree native to Indian sub-continent (Roxburgh,
1874). It has been used in Ayurvedic medicine for more than 4000 years due
to its medicinal properties. Most of the plant parts such as fruits, seeds,
leaves, bark and roots contain compounds with proven antiseptic, antiviral,
antipyretic, anti-inflammatory, antiulcer and antifungal uses. It has great
potential in the fields of pest management, environment protection and medicine.
Neem is a natural source of eco-friendly insecticides, pesticides and agrochemicals
(Brahmachari, 2004). Neem is considered to be a part
of Indias genetic diversity (Sateesh, 1998). It
is the most researched tree in the world and is said to be the most promising
tree of 21st century. The tree has adaptability to a wide range of climatic,
topographic and edaphic factors. It thrives well in dry, stony shallow soils
and even on soils having hard clay pan, at a shallow depth. Neem tree requires
little water and plenty of sunlight (Sateesh, 1998). The
tree grows naturally in areas where the rainfall is in the range of 450 to 1200
mm. However, it has been introduced successfully even in areas where the rainfall
is as low as 150 to 250 mm. Neem grows on altitudes up to 1500 m (Jattan
et al., 1995; Chari, 1996). It can grow well
in wide temperature range of 0 to 49°C (Hegde, 1995).
It cannot withstand water-logged areas and poorly drained soils. The pH range
for the growth of neem tree lies in between 4 to 10. Neem trees have the ability
to neutralize acidic soils by a unique property of calcium mining (Hegde,
Biologically active principles isolated from different parts of the plant include:
azadirachtin, meliacin, gedunin, salanin, nimbin, valassin and many other derivatives
of these principles. Meliacin forms the bitter principles of neem seed oil;
the seed also contain tignic acid (5-methyl-2-butanic acid) responsible for
the distincitive odour of the oil (Schmutterer, 1990;
Uko and Kamalu, 2001; Lale, 2002).
These compounds belong to natural products called triterpenoids (Limonoids).
The active principles are slightly hydrophilic, but freely lipophilic and highly
soluble in organic solvents like, hydrocarbon, alcohols, ketones and esters
(Schmutterer and Singh, 1995). Therefore, this review
will focus on the relevance of neem and its products in agriculture, industry,
biomedicine and environment.
ORIGIN AND DISTRIBUTION OF NEEM
Two species of Azadirachta have been reported, Azadirachta indica
A. Juss-native to Indian subcontinent and Azadirachta excelsa Kack.
confined to Philippines and Indonesia (Jattan et al.,
1995; Hegde, 1995). The former grows as a wild tree
in India, Bangladesh, Burma, Pakistan, Sri Lanka, Malaysia, Thailand and Indonesia.
Presently neem trees can be seen growing successfully in about 72 countries
worldwide, in Asia, Africa, Australia, North, Central and South America (Ahmed
et al., 1989; Sidhu, 1995; Sateesh,
1998; Fathima, 2004).
There are an estimated 25 million trees growing all over India (Rembold,
1996), of which 5.5% are found in Karnataka and it is in the third place
next to Uttar Pradesh (55.7%) and Tamilnadu (17.8%) occupying the first two
places respectively. The other states of India where neem tree is found growing
includes Andhra Pradesh, Assam, Bihar, Delhi, Gujarat, Haryana, Himachal Pradesh,
Kerala, Madhya Pradesh, Maharashtra, Meghalaya, Orissa, Punjab, Rajasthan, West
Bengal along with Andaman and Nicobar Islands, the Union territory (Sindhuveerendra,
1995; Chakraborthy and Konger, 1995; Bahuguna,
1997; Fathima, 2004). India stands first in neem seed
production and about 442,300 tons of seeds are produced annually yielding 88,400
tons of neem oil and 353,800 tons of neem cake.
TAXONOMICAL CLASSIFICATION OF NEEM
Neem is a member of the Mahogany family. It has similar properties to its close relative, Melia azederach. The word Azadirachta is derived from the Persian azaddhirakt (meaning 'noble tree). The taxonomic positions of neem are as follows:
Tribe - Melieae
Latin: Azadirachta indica
Indian: Holy tree, Indian lilac tree
Hindi: Neem, Nim
Hausa: Dogon yaro
Igbo: Ogwu akuma
BOTANICAL DESCRIPTION OF NEEM
It is a hardy, fast-growing evergreen tree with a straight trunk, long spreading
branches and moderately thick, rough, longitudinally fissured bark. Mature trees
attain a height of 7-15 m (23-50 feet) (Ogbuewu, 2008).
The tree starts producing the yellowish ellipsoidal drupes (fruits) in about
4 years, becomes fully productive in 10 years and may live for more than 200
years. The leaves are compound, imparipinnate, comprising up to 15 leaflets
arranged in alternate pairs with terminal leaflets (Ogbuewu,
2008). The leaflets are narrow, lanceolate, up to 6 cm long. The flowers
are abundant, sweet-smelling white panicles in the leaf axils. Seed propagation
in nurseries followed by direct planting in the field is the accepted method
to produce plantation stands (Ogbuewu, 2008). The one
seed neem fruit is yellow when ripe and is about one inch long (Ogbuewu,
2008). Neem flowers mature from May to August (Koul
et al., 2006) in India.
CHEMISTRY OF NEEM
Major chemical constituents of neem are Terpenes and Limonoids. The major active
components in the Limonoids are azadirachtin, 3-deacetyl-3- cinnamoylazadirachtin,
I-tigloyl-3-acetyl-II-methoxyazadirachtin, 22, 23-dihydro-23β-methoxyazadirachtin,
nimbanal, 3-tigloylazadirachtol, 3-acetyl-salannoV nimbidioV margocin, margocinin,
margocilin and others (Ogbuewu, 2008). Terpenoids are
isoazadirolide, 6 nimbocinolide, nimbonone, nibonolone, methylgrevillate and
Margosinone. Neem increases the production of Glutathione-s-transferase, thus
improving the ability of the liver to detoxify itself of chemical contamination.
NEEM AND ENVIRONMENTAL PROTECTION
Afforestation: The large scale plantation of neem trees help to combat
desertification, deforestation, soil erosion and to reduce excessive global
warming (Sateesh, 1998). Neem has high rate of photosynthesis
and liberates more oxygen than many other tree species, thus purifying the atmosphere
(Nigam et al., 1994). Neem products have water
purifying activity. Neem leaf powder could be used as biosorbent for the removal
of dyes like Congo red from water (Bhattacharyya and Sharma,
2004). The temperature under the neem has been found to be ~10°C less
than the surrounding temperature, during hot summer months in the northern parts
of India. In agro-forestry, neem product benefits extended to providing shade,
firewood, timber, wind breaks, shelter belt and check against desertification
in the semi-arid zone of northern Nigeria.
Neem has the ability to re-sprout after cutting and to re-grow its canopy after
pollarding. Thus it is highly suited for pole production. In Saudi Arabia neem
plantation when full grown is expected to provide shade to about two million
pilgrims (Ahmed et al., 1989). In Nigeria, neem
forms about 90% of the trees in the forestry plantations established in the
12 states within the savanna zone under the afforestation programme, Nigeria
inclusive (Nwokeabia, 1994). In Chad, neem constitutes
about 17% of the tree cover (Ohabuike, 1995). Neem plantations
have been used for halting the spread of Sahara desert in the countries from
Somalia to Mauritania.
PEST MANAGEMENT PROSPECTS
The dependency on synthetic chemicals during early and middle twentieth century
has prompted the large scale synthesis of newer chemicals (pesticides). Many
a times, the side effects of the synthetic pesticides are more serious than
problems themselves. They are also known to cause health problems in farmers
of both developed and developing countries. According to World Health Organization
estimation, annually 220,000 deaths occur due to acute poisoning caused by synthetic
pesticides (Sateesh, 1998).
Neem based pesticides are found to be much safer in this regard. Today, neem has gained importance internationally as all communities have inclined towards green technology. Neem products have no ill effects on humans and animals and have no residual effect on agricultural produce. This makes neem the best, reliable substitute to hazardous pesticides. The demand for chemical pesticides will be reduced by large scale use of neem based pesticides that will in turn reduce the load of synthetic chemicals in environment.
Today, modern societies, finding themselves confounded in the web of their
creation, are willing to revert to nature for remedies and neem tree provides
a promising means in this matter. Neem wood is durable and termite resistant
and thus used as mulch materials. The pesticidal activity of neem span a wide
spectrum, having repellent, phagodeterrent (antifeedant), insect growth regulatory
(IGR), anti-ovipositional, fecundity and fitness reducing properties on insects.
Schmutterer and Singh (1995) listed 413 insect pest species
sensitive to neem products. These principles act as ecdysteroid analogues, which
affect corpus cardiacum and block reproductive and growth processes in most
insects causing sterility in females and degenerative changes in male testis
due to disturbance in insect metabolism. Formulations like: Margosan O(R),
Neemix (), Azatin(R), NIM-20 and NIM-76, gave negative result
with respect to toxicity effect on mammals (Schmutterer,
1990; Govindachari et al., 2000). Hence,
Neemix (), was registered for use on vegetables in US for its
inherent safety. In most tests, neem products performed equally or sometimes
better than synthetics like Pirimiphos-methyl (Actellic 25 EC), Permethrin and
Lindane (γBHC) (Ogunwolu and Oddunlami, 1996; Lale
and Mustapha, 2000). Furthermore, the oriental yellow scale insects Aonidiela
orientalis threatens survival of this Jewel in the savanna (Mahmood,
1995), through necrosis, chlorosis and scorching of the whole foliage, as
they inject toxic metabolites into the foliage.
Neem based pesticides are easy to prepare, cheap and highly effective and thus
constitute an important source of pesticide for economically poor third world
country farmers (Brahmachari, 2004). Neem bio-pesticides
are systemic in nature and provide long term protection to plants against pests.
Pollinator insects, bees and other useful organisms are not affected by neem
based pesticides (Tanzubil, 1996).
Organic fertilizer prospects: Neem cake is a cheap and useful fertilizer.
The plant debris is potential source of organic manure (Brahmachari,
2004). Neem cake coated with urea has been produced, which when used increases
nitrogen assimilation compared to untreated urea. Neem leaves could be used
as a source for the preparation of vermi-compost having both fertilizer and
pesticidal potential (Gajalakshmi and Abbasi, 2004).
Biological Nitrogen Fixation (BNF) maintains soil nitrogen (N) fertility. Non-symbiotic
micro organisms like photosynthetic bacteria and Blue Green Algae (BGA) enhance
this process. It is also known to increase soil fertility and water holding
capacity. In field experiments neem cake stimulate algal growth by suppressing
the grazers particularly Ostracods (class: Crustacea). BGA biomass tripled and
N-fixation activity increases by 10 fold (Grant et al.,
1983). The underlying mechanism is acute toxic effect rather than anti-feedant
on the Ostracods. Admixing neem cake with urea fertilizer improves efficiency
of fertilizer utilization in crop production by gradual release of nitrogen
to crops (Ketkar, 1983). In tomato fields, neem cake
significantly reduce root-knot nematode index to zero, with improved growth
of tomatoes. The cake inhibited larval emergence and egg hatching. Finally these
effects of neem enable exploitation by Nigerian, Indians and other Asians.
NEEM AND AGRICULTURE
Animal feeds: The livestock industry in developing countries has been
plagued by numerous problems, which include scarce feed ingredients that are
in strict competition with mans dietary need. The high cost of conventional
feedstuff has already sent a lot of livestock farmers out of business, thus
leading to reduction in overall animal protein production and availability for
humans dietarys need. The provision of feed alone has been reported to
account for 60-80% of total cost of livestock production in developing countries
alone (Igboeli, 2000; Esonu, 2006).
In view of this, there is increased interest by livestock farmers on the search
for non conventional feed ingredients of comparable quality that are believed
to be cheaper such as leaf and seed meals of ethnomedicinal plants (Okoli
et al., 2001, 2002).
In an effort to develop new feedstuff for animal rearing, a number of researchers
in recent times has investigated the proximate composition of neem seed cake
(Bawa et al., 2006; Uko and
Kamalu, 2001) and leaf meal (Oforjindu, 2006; Esonu
et al., 2005, 2006; Ogbuewu
et al., 2010a, b) and its use as feedstuff
in poultry (Esonu et al., 2005; Oforjindu,
2006; Uko and Kamalu, 2007) and rabbits (Sokunbi
and Egbunike, 2000a; Ogbuewu, 2008). Result of proximate
analysis of neem showed that neem leaf meal had of 92.42% dry matter, 7.58%
moisture, 20.68% crude protein, 16.60% crude fibre, 4.13% ether extract, 7.10%
ash and 43.91% nitrogen free extract (Esonu et al.,
2005; Oforjindu, 2006; Ogbuewu,
Neem cake has also been very widely used as animal feed (Bawa
et al., 2006; Uko and 2007). Despite the
bitter components, livestock consume diets containing varied percentage of neem
cake. Alkali treatment of neem cake with caustic soda yields palatable product,
by removing the toxicant triterpenoids (Devakumar and Dev,
1993). Nagalakshmi et al. (1996) and Verma
et al. (1998) reported beneficial effect of alkali treated (10-20
g NaOH) neem kernel cake incorporated into poultry feeds. It resulted to an
increased feeding value and protein utilization with spectacular growth. However,
no significant difference was observed among the different dietary groups in
feed intake, egg production, egg quality, fertility, hatchability and chick
weight (Nagalakshmi et al., 1996; Verma
et al. 1998).
Neem oil and de-oiled neem seed cake are used as animal feed. Neem oil which
is rich in long chain fatty acids is used in poultry feed. Deoiled neem seed
cake is rich in essential amino acids, crude proteins, fiber contents, sulphur
and nitrogen (Uko and Kamalu, 2007).
Eco-friendly agrochemicals: Approximately one third of worlds
agricultural food stuffs get destroyed by more than 20,000 species of field
and storage pests and diseases (McEwen, 1978). To prevent
this loss, large amounts of synthetic pesticides are applied, out of which only
0.1% reaches the target pests and more than 99% contaminates the ecosystem (Sateesh,
1998). In addition, synthetic agrochemical usage has resulted in development
of resistant pests and pathogens. Cost-effective, non toxic, biodegradable,
eco-friendly and botanical soft-agro chemicals are the need of present day agriculture
as an alternative to hazardous and recalcitrant synthetic chemicals (Sateesh,
1998). Neem tops the list of 2,400 plant species that are reported to have
antimicrobial properties and is regarded as the most reliable source of eco-friendly
agrochemical property. Neem is also used as a bio-control agent to control many
plant diseases (Kak, 2000). The insecticides from neem
are non-phytotoxic with good shelf life and effective against a wide range of
insects and pests.
Neem products are effective against more than 350 species of arthropods, 12
species of nematodes, 15 species of fungi, 4 strains of viruses, 2 species of
snails and 1 crustacean species (Saxena et al., 1989;
Nigam et al., 1994; Singh
and Raheja, 1996; Mehta, 1997). Two tetracyclic
triterpenoids - meliantetyraolenone and odoratone isolated from neem exhibited
insecticidal activity against Anopheles stephensi (Siddiqui
et al., 2003). Over 195 species of insects are affected by neem extracts
and insects that have become resistant to synthetic pesticides are also controlled
with these extracts. The apprehension that large-scale use of neem based insecticides
may lead to resistance among pests, as being observed with synthetic pesticides,
has not been proved correct. This is because the neem based insecticides have
relatively weak contact effect in insects and also they have unique mode of
action on insects life cycle and physiology.
Today, many neem bio-pesticides are now in market world over (Khanna,
1992; Brahmachari, 2004). Commercially available
neem formulations like Achook (0.15% E.C.), Bioneem (0.03% E.C.), Nimbicidine
(0.03% E.C.) and Neemark (0.03% E.C.) showed antifungal activity against pathogenic
fungi viz., Fusarium oxysporum, Alternaria solani, Curvularia lunata,
Helminthosporium sp. and Sclerotium rolfsii (Bhonde
et al., 1999).
NEEM AND BIO-MEDICINE
Ethnoveterinary usage: In third world countries, neem has been used
for centuries to provide health cover to human and livestock in various forms.
In poultry, the bark is used to treat wounds, diarrhoea, ticks and lice (Ogbuewu,
2008). In the poultry industry, aflatoxicosis caused by Aspergillus flavus
which originates from contaminated poultry feed is prevented using neem
leaves. Neem leaf extract inhibits the production of aflatoxin by Aspergillus
parasiticus (Allameh et al., 2002) and Patulin
production by Penicillium expansum (Mossini et
al., 2004). The processed neem cake has wormicidal activity and can
be used as an excellent poultry feed. The leaves are used to treat abscesses
and also applied after castration (Ogbuewu, 2008). They
are also effective against bleeding, udder infections, fever, foot rot and lice
in ruminants. Neem leaf has anti-hyperglycaemic and hypocholesmic effects in
rabbits (Sokunbi and Egbunike, 2000b;
Ogbuewu et al., 2010a, b) and poultry (Oforjindu,
2006; Esonu et al., 2006).The seeds are used
for the treatment of ticks in ruminants and the bark, seeds, leaves and roots
are used as an insect repellent.
All parts of the plant, as well as the gum and oil, are effective against worms,
wounds in the mouth, glossitis, E. coli, bacillosis, swelling of the
liver, jaundice, bloody dysentery and intestinal wounds (Ketkar
and Ketkar, 1995). They are also used for constipation, indigestion, respiratory
and throat disorders, asthma, pleuropneumonia and swelling of the mucous membranes
in the respiratory tract and lungs. They are also used in skin disorders including
ringworm, alopecia, eczema, urticaria, scabies, ticks and lice. Other minor
indications include metritis, orchitis and tetanus, rinderpest, rheumatism,
stoppage of urination, swelling of the kidney, mastitis, otitis and abscess
in the ear (Ogbuewu, 2008). Alcohol and aqueous extracts
of flowers of neem exhibits lethal effect against cattle filarial parasite Setaria
cervi (Mishra et al., 2005). Livestock insects
such as maggots, horn flies, blow flies and biting flies are controlled traditionally
Neem and ethnomedicinal uses: In West Africa, India, Burma, etc., both
aqueous and alcohol extracts of bark and leaves of neem are effective anti malaria
agents, particularly on chloroquine resistant strains (Badam
et al., 1987; Udeinya et al., 2008).
One active component, gedunin gave significant control as effective as quinine
on malaria (Khalid et al., 1989; Subapriya
and Nagini, 2005). The mechanism is possibly redox status of red blood cells
(RBC) on parasite. The plasmodial parasite generates oxidant, while neem extracts
reduced the oxidized cells to destroy the malaria parasite.
Furthermore, neem barks and leaves posses strong antiseptic property warranting
use as active ingredient in tooth paste in India and Germany. While aqueous
extract of leaves exhibit laxative potentials by increased bowel movement (Uko
et al., 1995), over dose could however produce severe abdominal cramps
or rectal prolapse. Kloos and McCullough (1987) reported
potency of neem seed oil on snail fever (Schistosomiasis) with the active
principle being mulluscicidal, ovicidal and cercariacidal. Several herbalists
opined that neem products have broad spectral chemotherapeutic effect on the
Flat, Tape and Round worms (Devakumar et al., 1985).
Dental gel containing neem leaf extract reduces the oral plaque index and bacterial
count (Pai et al., 2004).
Neem is used to treat malarial fever in ayurvedic medicine system. Neem oil
treated mosquito nets and mosquito-repellent tablets are now available in the
North-east India. Gedunin (a Limonoids) obtained from neem has activity similar
to quinine against malarial pathogen. The neem liminoids (Azadirachtin, salannin,
deacetylgedunin) exhibited high larvicidal, pupicidal and anti-ovipositional
bioactivity against malaria vector - Anopheles stephensi. Tablet suspension
of the bark and leaf of neem showed moderate effect against malarial pathogen,
Plasmodium sp. (Isah et al., 2003). Methanolic
extract fraction of neem leaves when tried against Coxsackie B group viruses,
produced in vitro antiviral and virucidal effect (Badam
et al., 1999). Anticarcinogenic activity of neem leaf extract was
observed in murine system (Dasgupta et al., 2004).
Injection of neem leaf preparation to tumor in mice reduced tumour growth,
exhibiting anti-carcinogenic activity (Baral and Chattopadhyay,
2004). Induction of apoptosis in rat oocytes was seen when treated with
neem leaf extract (Chaube et al., 2006). Buccal
pouch carcinogenesis in hamsters was inhibited by ethanolic leaf extract of
neem (Subapriya et al., 2005). Good antioxidant
activity was observed with neem leaf aqueous extract; flower and stem bark ethanolic
extracts. Neem bark extract had potential of controlling gastric hyper-secretion
and gastro-esophageal and gastro-duodenal ulcers (Bandyopadhyayk
et al., 2004). Acetone-water neem leaf extract showed antiretroviral
activity through inhibition of cytoadhesion. The extract increased haemoglobin
concentration, mean CD4+ cell count and erythrocyte sedimentation rate in HIV/AIDS
patients (Udeinya et al., 2004). Enhancement
of antibody production and cellular mediated response by neem components helps
in the treatment of AIDS.
NEEM AND REPRODUCTION
The neem seed oil, leaf extracts and NIM-76 act as powerful spermicide and
significantly inhibited spermatogenesis, decreased sperm motility, count and
cessation of fertility. These conditions were reversed by the withdrawal of
neem products 4-6 weeks later (Sadre et al., 1983).
Ogbuewu et al. (2009) reported no significant
reduction in libido of rabbit bucks fed graded levels of neem leaf meal based
diets. Furthermore, neem seed oil possesses anti-implantation and abortifacient
properties. Sinha et al. (1984) found spermatozoa
of human and Rhesus monkey were immotile and die within 30 min of contact with
NSO in an intravaginal dose of 1.0 mL. Vaginal biopsy revealed no side effect,
while radio-isotope studies indicate non-absorption in the vagina and non anti-ovulatory
(Sinha et al., 1984). These findings enabled
neem oil formulation sensal use in India as powerful contraceptive.
Ogbuewu et al. (2009) and Mohan
et al. (1997) reported significant reduction in semen volume, sperm
count, higher incidence of morphological abnormalities of spermatozoa, fertilizing
ability of rabbit bucks fed neem leaf meal based diets and hatchability of eggs
on birds fed neem kernel cakes. The contraceptive property of neem oil has been
reported (Upadhyay et al., 1994). Neem leaf extract
has spermatotoxic effect (Ogbuewu et al., 2009).
The leaf extracts of neem showed 100% immobilization and mortality of human
spermatozoa at a 3 mg dose within 20 seconds (Khan and Awasthy,
2003; Khillare and Shrivastav, 2003). A new vaginal
contraceptive, NIM- 76 was developed from neem oil having antimicrobial activity
against Escherichia coli, Klebsiella pneumoniae and Candida albicans
(Ram et al., 2002). Treatment of mice with
neem leaf extract (aqueous) caused adverse effects on sperm motility, acrosomal
morphology and number of spermatozoa (Mishra and Singh,
2005). Neem seed extracts inhibited folliculogenesis in albino rats. Neem
extracts could thus be used as bio-rodenticides instead of toxic synthetic rodenticides
that are pollutants (Roop et al., 2005).
NEEM AND SERUM METABOLITES
Administration of 5 g of aqueous leaf extract or an equivalent amount of dried
leaf significantly reduced the insulin dosage by 30 - 50%, without a significant
effect on the blood glucose levels (Khosla et al.,
2000; Gupta et al., 2008). Aqueous extract
of neem root and leaves reduced blood sugar level in rats exhibiting antidiabetic
activity (Halim, 2003). In a study with rabbits and
guinea pigs, it was reported that administration of the leaf extract of Azadirachta
indica induced a potent and dose-dependent hypotension in rabbits (5-200
mg kg-1, IP) and guinea pigs (5-40 mg kg-1). The extract
also exhibited anti-carrhythmic activity (40 mg kg-1 IV) against
ouabain-induced dysrhythmia in rabbits. The mechanism of action may be explained
an effect on vascular smooth muscle, giving rise to vasodilatation.
The neem leaf extract provided a hepatoprotective effect against paracetamol-induced
hepatic cell damage in rats, findings supported by histopathological studies
(Gupta et al., 2008). Both the neem leaf extract
and seed oil produced a hypoglycaemic effect in normal as well as diabetic rabbits,
comparable to that of glibenclamide. The effect was more pronounced in the diabetic
animals. Pretreatment with Indica leaf extract or seed oil, given two
weeks prior to alloxan, partially prevented the rise in blood glucose levels
as compared to control diabetic animals (Gupta et al.,
Administration of aqueous extract of neem along with DOCA salt prevented the
development of hypertension in rats (Obiefuna and Young,
2005). Administration of the mature leaf extract decreased serum cholesterol
significantly without changing serum protein, blood urea and uric acid levels
in rats (Chattopadhyay et al., 2000; Ogbuewu
et al., 2010a, b).
Neem and anti-microbial activity: Several active principles from neem
have demonstrated high efficacy, against most pathogens. As fungicides, over
14 common fungi species are sensitive to neem preparations (Khan
and Wassilew, 1987) they include the genera Trichophyton (athletes
foot), Epidermophyton (ringworm of skin and nails), Microsporum (ringworm
of skin and hair) and Candida (thrush). SaiRam et
al. (1997) reported protection against systemic candidiasis (Candida
albican) by NIM-76. The mechanism is simply antifungal and immunomodulatory.
In Aspergillus flavus, neem leaf extract fail to inhibit growth, but
reduce formation of aflatoxin by blocking polyketides production, which is commonly
converted to toxins. Several diseases including Cercospora, Anthracnose, Downy
mildew and Sigatoka are under investigation to establish efficacy of neem products
as plant fungicides. As antibiotics, pathogenic bacteria like Staphylococcus
aureus, Salmonella typhi are significantly suppressed by NSO. Trials with
NIM-76 significantly suppressed E. coli and K. pneumoniea, which
hitherto were insensitive to whole NSO (Ram et al.,
2002). As antiviral agents, experiment with Small pox, Chicken pox and Fowl
pox viruses show biological efficacy of neem extracts. Crude neem extracts adsorbed
the viruses by blocking entry into uninfected cells. NIM-76 suppressed Polio
virus replications and inhibited DNA polymerase of Herpes virus with no potency
once infection is established in vivo (Rao et
al., 1989). Therefore neem can be used against phytopathogenic fungi
as a means of biological control (Jatav and Mathur, 2005).
Neem leaf and seed extracts exhibited antidermatophytic activity against dermatophytes
viz., Trichophyton ruberum, Mentagrophytes, Trichophyton violaceum,
Microsporum nanum and Epidermophyton flocosum under in vitro conditions
(Natarajan et al., 2002). Neem seed oil showed
bactericidal activity against 14 strains of pathogenic bacteria (Baswa
et al., 2001). Crude aqueous and solvent extracts of neem were tried
against 20 strains of pathogenic bacteria wherein crude extract produced better
results (Srinivasan et al., 2001). Neem leaf
extracts are antimutagenic. The ethanolic extract of neem leaves exhibited strong
antimutagenic activity in Channa punctatus, a fresh water fish model
(Farah et al., 2006).
In 2002, at the World neem conference, idea of promoting neem as an Industrial
Plant was put forward (Kumar, 2003). Several industries
including pharmaceuticals, cosmetics and textile industries use neem oil (Jattan
et al., 1995). Many such neem-based commercial preparations are currently
available (Khanna, 1992). In India neem is highly exploited
by many Ayurvedic drug industries. Neem oil and powdered neem leaves are employed
in various cosmetic preparations such as face creams, nail polish, nail oils,
shampoos, conditioners (Jattan et al., 1995).
A new shampoo, based on seed extract of neem was highly effective, more than
permethrin-based product, against head lice under in vitro conditions
(Heukelbach et al., 2006). Neem cake a by-product
of neem oil industry is used as livestock feed, fertilizer and natural pesticide.
Neem oil is commonly used in soap production. Medicated neem soaps are gaining
Neem based toothpaste is widely used in India and European countries. Neem
is a source for many oral-hygiene preparations and dental care products. Neem
bark yields gum and tannins which are used in tanning, dyeing etc. Neem seed
pulp is used as a rich source of carbohydrate in fermentation industries and
for methane gas production. Cultivation of neem and processing of neem products
provides employment and income generation opportunities. Collection of neem
seeds to be supplied to the industries provides important means of supplementary
employment and income for the poor households, especially the rural women. India
stands first in neem production and about 540,000 tons of seeds are produced
annually yielding 107,000 tons of neem oil and 425,000 tons of neem cake. The
amount of azadirachtin available is estimated to be about 1600 tons per annum,
providing enormous amount of raw material for pesticide industry. In the product
sector, annual estimated turnover is about Rs. 1000-1200 crores. Small scale
industries have a major role to play in harnessing the potential. Therefore,
in India it is the time to take right steps in promoting neem, both for the
benefit of farmers and industries (Kumar, 2003).
NEEM AND PATENCY
During the past five decades intensive investigations on the diverse properties of neem have been carried out. As a result large numbers of research publications and books have been published. Many conferences have been conducted at national and international level. Hundreds of active compounds that are isolated from various parts of neem find their applications in pesticide, medicine, health care and cosmetic industry all over the world. World over the neem tree has been recognized as a commercial opportunity.
Many neem related processes and products have been patented in Japan, USA and
European countries, since 1980s. In 1983, Temuro Corporation obtained the first
US patent for its therapeutic preparation from neem bark. USA with 54 patents
on neem and neem based products stands first followed by Japan (McEwen,
1978), Australia (Chakraborthy and Konger, 1995)
and India (Fathima, 2004). Since 1995, more than 53 patent
applications are pending in India for either gazette notification or opposition.
Hopefully, if all these patents are granted India will have the largest number
of patents in neem. Majority of patents that have been granted are for crop
protection application (63%) followed by health care (13%), industrial (5%),
veterinary care (5%), cosmetics (6%) and others (8%). Organization wise, largest
number of patents are owned by Certis-W.R. Grace followed by Rohm and Haas (Kak,
2000), CSIR-India (Fathima, 2004), Trifolio (Jattan
et al., 1995), Bayer (Chari, 1996) and EID
Parry (Brahmachari, 2004).
Owing to its versatile characteristics neem is rightly called the village pharmacy. National Research Council, Washington, USA considers the neem, one of the most promising of all plants and the fact is that it may eventually benefit every person on this planet. Probably no other plant yields as many strange and varied products or has as many exploitable by-products. However, most of these findings on neem and its products are not patented, despite being potentially valuable, cost effective, reduces incidence of pests and parasite resistance with increase in agricultural production, environmental protection and health care services for humans and livestock. It is inferred from the above that neem plant is indeed a jewel globally. In line with these adequate benefits, researches on neem must be directed at identification and quantification of the active principles and patenting of findings thereby making these findings readily accessible to mankind for adoption.
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