Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 

Nutritional Composition and Anti-Nutrient Content of Elephant Foot Yam (Amorphophallus campanulatus)



Theresia Nur Indah Koni, Rusman , Chusnul Hanim and Zuprizal
 
ABSTRACT

Background and Objective: The nutrient and anti-nutrient compositions of a foodstuff affects its utilization as food or feed. The nutrient contents of food ingredients are affected by the environmental conditions in which the plant was grown and oxalate in a foodstuff limits its utilization and exerts a negative effect on consumers. The aim of this study was to evaluate the nutrient value, minerals and anti-nutrient values in Amorphophallus campanulatus (A. campanulatus). Materials and Methods: The A. campanulatus tubers were collected from East Nusa Tenggara, Indonesia and then cleaned, peeled, chopped, sun-dried for 2 days (at 30-32°C), milled into powder and then analyzed for the proximate and anti-nutrient contents. Results: The nutrient content of A. campanulatus flour included crude protein (1.126±0.101%), crude fat (1.173±0%) and crude fiber (3.447±0.142%). The detected minerals included P (1443.33±34.185 mg kg–1), Ca (8535.76±543.75 mg kg–1) and Mg (1512.39±89.28 mg kg–1). Anti-nutrient analysis indicated the presence of oxalates (318.51±3.2 mg kg–1), tannins (0.46±0.04%), cyanide (35878±0.402 ppm) and phytates (0.165±0.015%). Conclusion: These results revealed that A. campanulatus was high in mineral content but low in anti-nutrient content, so it can be used for food or feed.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Theresia Nur Indah Koni, Rusman , Chusnul Hanim and Zuprizal , 2017. Nutritional Composition and Anti-Nutrient Content of Elephant Foot Yam (Amorphophallus campanulatus). Pakistan Journal of Nutrition, 16: 935-939.

DOI: 10.3923/pjn.2017.935.939

URL: https://scialert.net/abstract/?doi=pjn.2017.935.939
 
Received: June 14, 2017; Accepted: November 13, 2017; Published: November 15, 2017


Copyright: © 2017. This is an open access article distributed under the terms of the creative commons attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

INTRODUCTION

Amorphophallus campanulatus is synonymous with Amorphophallus paeoniifolius (A. paeoniifolius) and is commonly known as the elephant foot yam and also called as maek in East Nusa Tenggara, Indonesia. The plants are herbaceous and belong to the Araceae family, which is a native crop of South Asia that is widely distributed in India, Malaysia, the Philippines, Bangladesh, Indonesia and Southeast Asia1-4.

Elephant foot yam has been cultivated as an intercrop plant along with ginger under coconut or banana trees in India. The production yield of the elephant foot yam is 50-80 t ha–1, thus, this plant has a low rate of production and is an underutilized crop in Indonesia4. The production of Amorphophallus muelleri (A. muelleri) in East Java is 6-10 t ha–1/year1 of fresh tuber. In East Nusa Tenggara, this plant is not cultivated (i.e., it is a wild plant), which is the cause of its low productivity. Elephant foot yam tubers are usually eaten as vegetables after boiling1. The tubers have been used as traditional food sources in Malaysia, Philippines, Bangladesh, Indonesia and India1-3 as traditional medicine1 and animal feed2. Elephant foot yam tubers contain P (34 mg/100 g), calcium (50 mg/100 g), vitamin A (434 IU/100 g)1, crude protein (2.14%), fat (0.46%), calcium (32.1 mg/100 g) and crude fiber (1.68%)5. The tuber also contains anti-nutrient factors, such as oxalate and phytate. The level of oxalic acid in the elephant foot yam is 1.3%2. The nutrient compositions of elephant foot yam tubers vary according to where they are grown, the soil, the season, the water and climate situations6. This study was conducted to evaluate the nutrient and anti-nutrient compositions of wild elephant foot yams from East Nusa Tenggara, Indonesia.

MATERIALS AND METHODS

Materials: Elephant foot yam tubers were collected from Kupang District of East Nusa Tenggara, Indonesia. The tubers were cleaned, peeled, chopped to 7 cm in length, sun-dried for 2 days at 30-32°C and milled into powder.

Nutrient contents: The flour of elephant foot yam tubers was analyzed for proximate, energy, mineral contents and the anti-nutrient factors, such as hydrogen cyanide, oxalate, tannins and phytate.

The proximate and mineral compositions were determined according to the AOAC7 methods. First the samples were oven dried at 105°C to assess the moisture content (method 934.01), the Kjeldahl method, which consists of digestion, distillation and titration (method 990.02) was utilized to assess the crude protein (nitrogen×6.25) content and extraction in acid and alkali solutions (method 978.10) was used to assess the crude fiber. The mineral contents, including calcium (Ca), magnesium (Mg) and phosphorus (P), were measured by dissolving ash samples in acids (a mixture of HCl and HNO3). Subsequently, Ca and Mg were assessed using the AAS (method 942.05) and P was determined using spectrophotometry method (method 965.17). The gross energy value was determined with a bomb calorimeter.

Anti-nutrient contents: The anti-nutritional factor oxalic acid was assessed by HPLC according to the methods of Savage et al.8. Phytate was evaluated with spectrophotometry method according to Vaintraub and Lapteva9, tannins were determined according to the method of Burns10 and hydrocyanic acid (HCN) content was determined using the alkaline titration method (method 915.03)7.

Statistical analysis: Three independent analyses were performed for nutritional components, minerals and anti-nutrient contents. The results are expressed as the means and the standard deviation values (mean±SD).

RESULTS AND DISCUSSION

Nutritional composition: Elephant foot yam is wild crop in East Nusa Tenggara and its nutritional content is presented in Table 1. Protein content of the tuber was 1.126%, which falls within the range of 0.84-2.6% of the protein contents of cultivar tubers of elephant foot yams in India as reported by Chattopadhyay et al.6. Paul et al.2 reported that elephant foot yams are rich in minerals but poor in proteins. The elephant foot yams examined in this experiment contained a higher content of crude fat than the elephant foot yam cultivars in India (0.01-0.4%) as reported by Chattopadhyay et al.6.

The phosphorus content of the yams was 1443.33 mg kg–1. Chattopadhyay et al.6 reported a similar value for elephant foot yams in India (20.89- 247 mg/100 g). The calcium content was found to be 8535.76 mg kg–1 and the magnesium content of this tuber was 1512.28 mg kg–1. Paul et al.2 reported that elephant foot yams are rich in minerals, such as calcium (950 mg/100 g), phosphorus (934 mg/100 g) and iron (0.6 mg/100 g). The elephant foot yam is a good source of minerals that can supply a large portion of the daily requirements of minerals in food or feed. Calcium and phosphorus are the major minerals that the body requires in large quantities.

Table 1:Analysis of the composition of Amorphophallus campanulatus
The values are presented as the means±the standard deviations of three determinations

Table 2:Anti-nutrient content of Amorphophallus campanulatus
The values are presented as the means±the standard deviations of three determinations

Minerals have functions in the body that include as enzymatic regulation, acid-base processes, bone growth and muscle stimulation.

Anti-nutrient content: Anti-nutrient factors can influence animals in several manners, including directly intoxicating the animals, causing mortality or decreased production11 and decreasing feed intake12. The mean anti-nutrient concentrations in the elephant foot yams in this study are provided in Table 2. The oxalate concentration was 31.851 mg/100 g, which is higher than that reported for cultivars in India (2.91-18.50 mg/100 g)6. The oxalate content in plants is affected by many factors, including the season, soil, water, climatic conditions and where the plant is grown13. Oxalic acid is strongly oxidized and exhibits mineral chelating activity. Oxalates react with calcium to produce insoluble calcium oxalate, which reduces calcium absorption11. A high intake of oxalate in foods causes hypocalcemia and the deposition of calcium oxalate crystals in the kidney2,14,15. Oxalic acid ingestion results in gastric hemorrhaging, corrosion of the mouth and gastrointestinal tract and renal failure16. Soluble oxalate ingestion of less than 2% for ruminants and 0.5% for non-ruminants might be acceptable14. Cattle and sheep are less affected because of the degradation of oxalate in the rumen17. In humans, the minimum dose of oxalate that can cause death in adults is 40-50 mg14. Cooking and fermenting can decrease the oxalate contents of soybean and soybean products18. Sun drying processes can also decrease the oxalate content by 26-35% in the false yam tuber.

Tannins are plant polyphenols that have the ability to form complexes with metal ions and macromolecules such as proteins and polysaccharides19. The tannin content in the elephant foot yams was 0.456% (Table 2). A. paeoniifolius extract contains flavonoids, tannins, proteins and carbohydrates20. Tannins have a strong affinity for proteins and form protein-tannin complexes21, which have been reported to be responsible for decrease in feed intake, growth rate, feed efficiency, net metabolizable energy and protein digestibility22. At the levels of 100-120 g kg–1, tannins reduce gastrointestinal parasitism in lambs23 and inhibit the growth of fiber-degrading bacteria in the digestive tracts of ruminants24. Vohra et al.25 reported that chicks fed tannins at dietary levels of 0.64-0.84% and 1.0-2% exhibited depressed growth, egg production and an increase in the content to >3% caused mortality. Tannin levels can be reduced by fermentation processes. Ridla et al.26 studied fermented Chromolaena odorata in rumen content and found that putak meal can reduce tannin levels.

Hydrogen cyanide was observed at a level of 35.878 ppm in the elephant foot yams (Table 2). Hydrogen cyanide (HCN) is widespread in the plant kingdom and mainly exists in the form of cyanogenic glucosides21. The cyanide concentration of the elephant foot yam is lower than that of the cassava root in which the level varies from approximately 75-350 ppm27 but can reach 1000 ppm or more21 depending on the variety, plant age, soil conditions, fertilizer application, weather and other factors11. High levels of HCN have been implicated in cerebral damage and lethargy in animals and humans. Cyanide toxicosis is caused by the inhibition of cytochrome oxidase, which is a terminal respiratory enzyme in all cells. When cytochrome oxidase is inhibited, the cells suffer from rapid ATP deprivation. Signs of cyanide toxicosis include labored breathing, excitement, gasping, staggering, convulsions, paralysis and death. Blood is bright red due to its high oxyhemoglobin content11. Highly toxic hydrocyanic acid (HCN) is released from cyanogenic glucosides during hydrolysis by the enzyme linamarase, which is present in the root peel of the cassava27. The lethal dose of HCN for humans is between 0.5 and 3.5 mg for an adult, depending on body weight and nutritional status and is between 30 and 210 mg kg–1 body weight21, while the lethal dose of HCN for cattle and sheep is 2.0-4.0 mg kg–1 body weight11. Cyanogens can be removed by drying, soaking and fermentation processes21. Hay and silage should be properly cured to ensure the loss of a majority of their cyanogenetic contents before being fed to livestock28. Phytic acid acts as strong chelator that forms protein and mineral compounds and is a common storage form for phosphorus in seeds21. Phytic acid chelates with minerals to produce phytates29. In elephant foot yams, the phytate content is 0.165%, which is higher than that of cassava root chips (0.09%) and sweet potatoes (0.1%)30 but lower than that in some seeds, such as sorghum (5.9-11.8 mg g–1), soybeans (9.2-16.7 mg g–1)31 and the false yam tuber (0.39%)32. Phytic acid has a strong affinity for binding metal ions, such as phosphorus and zinc ions and this leads to interference in the absorption of these minerals in the small intestine and affects various metabolic processes21. The phosphorus in phytic acid is not nutritionally available to monogastric animals. Dietary phytate forms insoluble phytate-mineral complexes and reduces the bioavailability of minerals. The small intestine of the human is devoid of phytate-degrading enzymes and the microbial population in the upper part of the digestive tract is also limited31. A phytate intake of 4-9 mg/100 g of food can decrease the Fe absorption by 4-5 fold in humans. The mean phytate level in the elephant foot yams was lower than the recommended level of 25 mg/100 g in food33. The phytate in the vegetables can be reduced by increasing temperature and heating time and the phytate content is reduced by 51% in sun-dried false yams32 and by 11-25% in Pterocarpus mildbraedii following heating at 90°C33.

CONCLUSION

According to the described results, the elephant foot yam is a good source of minerals and has a high caloric content. Thus, the elephant foot yam can be used for food or feed but because of its antinutritional value, it must be processed before consumption.

SIGNIFICANCE STATEMENT

This study reveals that the nutrient and anti-nutrient contents of the foodstuff like A. campanulatus indicate that this plant could serve as a nutritionally beneficial food-/foodstuff. This study will help researchers to uncover critical aspects of the nutrient composition of A. campanulatus, which is grown in East Nusa Tenggara, Indonesia, that many researchers have not previously been able to explore. Thus, new information about the nutrient and anti-nutrient contents of A. campanulatus may be discovered.

ACKNOWLEDGMENTS

This study was supported by the Hibah Disertasi Doctor project 2017 from the Directorate General of Higher Education, Ministry of Riset, Technology and Higher Education of the Republic of Indonesia.

REFERENCES
AOAC., 2005. Official Methods of Analysis of the Association of Official Analytical Chemistry. 18th Edn., AOAC International, Washington, USA.

Aganga, A.A. and S.O. Tshwenyane, 2003. Feeding values and anti-nutritive factors of forage tree legumes. Pak. J. Nutr., 2: 170-177.
CrossRef  |  Direct Link  |  

Allison, M.J., H.M. Cook and K.A. Dawson, 2001. Selection of oxalate-degrading rumen bacteria in continuous cultures. J. Anim. Sci., 58: 810-816.
CrossRef  |  Direct Link  |  

Burns, R.E., 1971. Method for estimation of tannin in grain sorghum. Agron. J., 63: 511-512.
CrossRef  |  Direct Link  |  

Burritt, E.A. and F.D. Provenza, 2000. Role of toxins in intake of varied diets by sheep. J. Chem. Ecol., 26: 1991-2005.
CrossRef  |  Direct Link  |  

Chattopadhyay, A., B. Saha, S. Pal, A. Bhattacharya and H. Sen, 2009. Quantitative and qualitative aspects of elephant foot yam. Int. J. Vegetable Sci., 16: 73-84.
CrossRef  |  Direct Link  |  

Cheeke, P.R. and L.R. Shull, 1985. Natural Toxicants in Feeds and Poisonous Plants. AVI Publishing Co., USA.

Cheeke, P.R., 1995. Endogenous toxins and mycotoxins in forage grasses and their effects on livestock. J. Anim. Sci., 73: 909-918.
CrossRef  |  Direct Link  |  

De Bruyne, T., L. Pieters, H. Deelstra and A. Vlietinck, 1999. Condensed vegetable tannins: Biodiversity in structure and biological activities. Biochem. Systemat. Ecol., 27: 445-449.
CrossRef  |  Direct Link  |  

Deshpande, S.S., S.K. Sathe and D.K. Salunkhe, 1984. Chemistry and safety of plant polyphenols. Adv. Exp. Med. Biol., 177: 457-462.
PubMed  |  Direct Link  |  

Eeckhout, W. and M. De Paepe, 1994. Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs. Anim. Feed Sci. Technol., 47: 19-29.
CrossRef  |  Direct Link  |  

Egekeza, J.O. and F.W. Qehme, 1980. Cyanides and their toxicity: A literature review. Tijdschr. Diergeneeskd., 105: 104-114.
PubMed  |  Direct Link  |  

Garcia, M., 1999. Cassava root meal for poultry. J. Applied Poult. Res., 8: 132-137.
Direct Link  |  

Giardina, S., C. Scilironi, A. Michelotti, A. Samuele, F. Borella, M. Daglia and F. Marzatico, 2014. In vitro anti-Inflammatory activity of selected oxalate-degrading probiotic bacteria: Potential applications in the prevention and treatment of hyperoxaluria. J. Food Sci., 79: 384-390.
CrossRef  |  Direct Link  |  

Kumar, V., A.K. Sinha, H.P.S. Makkar and K. Becker, 2010. Dietary roles of phytate and phytase in human nutrition: A review. Food Chem., 120: 945-959.
CrossRef  |  Direct Link  |  

Kurniawati, N., A. Meryandini and T.C. Sunarti, 2016. Introduction of actinomycetes starter on coffee fruits fermentation to enhance quality of coffee pulp. Emirates J. Food Agric., 28: 188-195.
Direct Link  |  

Makkar, H.P.S., P. Siddhuraju and K. Becker, 2007. Plant Secondary Metabolites. Humana Press Inc., Totowa, NJ., USA., ISBN-13: 9781597454254, Pages: 130.

Noonan, S.C. and G.P. Savage, 1999. Oxalate content of foods and its effect on humans. Asian Pac. J. Clin. Nutr., 8: 64-74.
CrossRef  |  Direct Link  |  

Paul, K.K., M.A. Bari, S.M.S. Islam and S.C. Debnath, 2013. In vitro shoot regeneration in elephant Foot Yam, Amorphophallus campanulatus Blume. Plant Tissue Cult. Biotechnol., 23: 121-126.
Direct Link  |  

Peetabas, N., R.P. Panda, N. Padhy and G. Pal, 2015. Nutritional composition of two edible aroids. Int. J. Bioassays, 4: 4085-4087.
Direct Link  |  

Rahman, M.M. and O. Kawamura, 2011. Oxalate accumulation in forage plants: Some agronomic, climatic and genetic aspects. Asian-Aust. J. Anim. Sci., 24: 439-448.
CrossRef  |  Direct Link  |  

Rahman, M.M., R.B. Abdullah and W.E.W. Khadijah, 2013. A review of oxalate poisoning in domestic animals: Tolerance and performance aspects. J. Anim. Physiol. Anim. Nutr., 97: 605-614.
CrossRef  |  Direct Link  |  

Ravi, V., C.S. Ravindran and G. Suja, 2009. Growth and productivity of elephant foot yam (Amorphophallus paeoniifolius (Dennst.) Nicolson): An overview. J. Root Crops, 35: 131-142.
Direct Link  |  

Ridla, M., Y.M. Mulik, I. Prihantoro and M.L. Mullik, 2016. Decreasing of total tannins from Chromolaena odorata silage with the additives of putak meal and rumen content. Buletin Peternakan, 40: 165-169.

Santosa, E., A.D. Susila, A.P. Lontoh, A. Noguchi, K. Takahata and N. Sugiyama, 2016. NPK fertilizers for elephant foot yam (Amorphophallus paeoniifolius (Dennst.) Nicolson) intercropped with coffee trees. Indonesian J. Agron., 43: 257-263.
CrossRef  |  Direct Link  |  

Santosa, E., S. Halimah, A.D. Susila, A.P. Lonto, Y. Mine and N. Sugiyama, 2014. KNO3 application affect growth and production of Amorphophallus muelleri blume. Indonesian J. Agron., 41: 228-234.
Direct Link  |  

Savage, G.P., L. Vanhanen, S.M. Mason and A.B. Ross, 2000. Effect of cooking on the soluble and insoluble oxalate content of some New Zealand foods. J. Food Compos. Anal., 13: 201-206.
CrossRef  |  Direct Link  |  

Shimi, G. and H. Haron, 2014. The effects of cooking on oxalate content in Malaysian soy-based dishes: Comparisons with raw soy products. Int. Food Res. J., 21: 2019-2024.
Direct Link  |  

Singh, A. and N. Wadhwa, 2014. A review on multiple potential of aroid: Amorphophallus paeoniifolius. Int. J. Pharm. Sci. Rev. Res., 24: 55-60.
Direct Link  |  

Udousoro, I.I. and E.B. Akpan, 2014. Changes in anti-nutrients contents of edible vegetables under varied temperature and heating time. Curr. Res. Nutr. Food Sci. J., 2: 146-152.
CrossRef  |  Direct Link  |  

Umoh, E.O., 2013. Ant nutritional factors of false yam (Icacina trichantha) flour. Internet J. Food Safety, 15: 78-82.
Direct Link  |  

Vaintraub, I.A. and N.A. Lapteva, 1988. Colorimetric determination of phytate in unpurified extracts of seeds and the products of their processing. Anal. Biochem., 175: 227-230.
CrossRef  |  Direct Link  |  

Vohra, P., F.H. Kratzeo and M.A. Joslyn, 1966. The growth depressing and toxic effects of tannins to chicks. Poult. Sci., 45: 135-140.
CrossRef  |  Direct Link  |  

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract