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Effect of Dietary Supplementation Based on an Ammoniated Palm Frond with Direct fed Microbials and Virgin Coconut Oil on the Growth Performance and Methane Production of Bali Cattle



Heni Suryani, M. Zain, R.W.S. Ningrat and N. Jamarun
 
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ABSTRACT

Objective: The objectives of this study were to evaluate the effect of supplementation of an ammoniated palm frond-based diet with Direct-Fed Microbials (DFM) and Virgin Coconut Oil (VCO) on the in vivo methane production, Dry Matter Intake (DMI), Organic Matter Intake (OMI), Daily Gain (ADG) and nitrogen retention of Bali beef cattle. Materials and Methods: The DFMs used in this study were Saccharomyces cerevisiae (SC) and Bacillus amyloliquefaciens (BA) and the Virgin Coconut Oil (VCO) contained 51.95% C12:0. In a pilot study, 16 male Bali cattle were assigned treatments in a randomized complete block design. Cattle were fed a basal diet containing (dry matter basis) 40% ammoniated palm frond and 60% concentrate and the treatments were: a) control, b) SC 1% DM, c) SC 0.5% DM + BA 0.5% DM and d) SC 1% DM+VCO 2% DM. Data were analysed by analysis of variance (ANOVA) and differences among means were tested using Duncan’s multiple range test (DMRT). Results: The results showed that supplementation with SC, SC+BA, SC+VCO significantly (p<0.05) reduced DMI and OMI but that the treatments were also able to increase ADG by 0.63, 0.63 and 0.71 kg day–1, respectively. Supplementation with SC+VCO increased the feed efficiency and reduced methane gas production by up to 20.63% compared to the control and nitrogen retention tended to decrease with DFM and VCO supplementation. Conclusion: These results suggest that supplementation with SC+VCO generates the best results in Bali beef cattle growth performance, methane gas production and feed efficiency.

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  How to cite this article:

Heni Suryani, M. Zain, R.W.S. Ningrat and N. Jamarun, 2017. Effect of Dietary Supplementation Based on an Ammoniated Palm Frond with Direct fed Microbials and Virgin Coconut Oil on the Growth Performance and Methane Production of Bali Cattle. Pakistan Journal of Nutrition, 16: 599-604.

DOI: 10.3923/pjn.2017.599.604

URL: https://scialert.net/abstract/?doi=pjn.2017.599.604
 
Received: April 15, 2017; Accepted: June 22, 2017; Published: July 15, 2017


Copyright: © 2017. This is an open access article distributed under the terms of the creative commons attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

INTRODUCTION

The area under oil palm plantation in Indonesia is increasing annually1 and palm frond is an agricultural by-product with the potential to be used as feed for ruminants2. The efficiency of rumen fermentation can be improved through a variety of approaches, including pre-treatment of the feed materials that will be given to cattle, the use of methanogenesis inhibitors and supplementation using microorganisms3-5. Direct-Fed Microbials (DFMs) are live microorganisms6 and the direct fed microbial Saccharomyces cerevisiae can compete with bacterial starch to stimulate growth factors, such as organic acids or vitamins, thus stimulating the population of cellulolytic enzymes7,8. Thus the digestibilities of dry matter, organic matter and fibre can be improved and rumen fermentation can be increased when high-concentrate diets are supplemented with live Yeast Culture (YC)9. Therefore, supplementation with SC could improve nutrient digestibility and cattle growth performance10. Additionally, the DFM Bacillus amyloliquefaciens is cellulolytic and can be degraded crude fibre and produce the extracellular enzymes cellulase and hemicellulase11. Supplementation of an ammoniated palm frond-based diet with SC plus BA could increase dry matter and organic matter digestibility in vitro12.

High fibre feed not only lowers the feed efficiency but also increases methane (CH4) production in ruminants. The release of methane causes the loss of 6-13% of the energy in the feed13. However, Medium Chain Fatty Acids (MCFA) have the potential to suppress ruminal methanogenesis14 and the reduction in archaeal numbers might be partially due to the defaunating effect of MCFA15. Virgin Coconut Oil (VCO) contains a high percentage of MCFA [7.19-8.81% C8:0, 5.65-6.59% C10:0, 46.9-48.0% C12:0 and 16.2-18.9% C14:0]16 but supplementation of the diets of lactating dairy cow with VCO at 29.8 g kg–1 DM do not affect the ruminal population of protozoa and methanogenic archaea17. Suryani et al.12 found that supplementation with SC+VCO could decrease the proportion of acetate propionate and methane gas production by up to 45% compared to a control ammoniated palm frond-based diet in vitro.

We hypothesized that DFM and VCO supplementation would increase the growth performance of Bali cattle by reducing CH4 production. Hence, the purpose of the current study was to evaluate the effect of DFM and VCO supplementation on feed intake, methane production and nitrogen retention in Bali cattle.

MATERIALS AND METHODS

The experiment was conducted from May-June, 2016, at the farm of the Animal Science Faculty of Andalas University, Indonesia.

Diet: Oil palm fronds without leaves and sticks that were previously treated with 6% urea were obtained from oil palm plantation of Andalas University, Indonesia. The DFMs used in the study were Saccharomyces cerevisiae and Bacillus amyloliquefaciens. The concentration of the live Saccharomyces cerevisiae of strain Meyen ex Hansen was 14×108colonies g–1. The Bacillus amyloliquefaciens obtained commercially11 at a concentration of 12×106 colonies g–1. Virgin coconut oil was obtained from Andalas University, Indonesia and it consisted of 9.23% C8:0, 7.22% C10:0 and 51.95% C12:0. The basal diet contained (dry basis) 40% ammoniated palm frond and 60% concentrate. The compositions of the feeds including the concentrate are shown in Table 1.

Experimental design and procedure: Sixteen male Bali beef cattle of similar age (1.5-2 years) and initial body weight (110-174 kg) were placed in individual pens with free access to water and feed. The animals and treatments were randomly assigned in Randomized Complete Block Design (RCBD) with four replicate blocks and four treatment blocks, the treatments are shown in Table 1.

The feed was offered during the morning and afternoon. The experimental period was 26 days, twenty one of which were for adaptation and five for samplings. The offered and rejected feed was weighed to determine the total intake of DM and OM. During the collection period, the animals were fitted with bags with a harness to collect the faeces. The total faecal excretion was collected once daily and representative samples (10% of the total) were dried at 60°C overnight and kept in sealed bags until analysis. The feed and faecles were ground to pass through a 1 mm screen and formed into a composite sample. Dry matter, organic matter and nitrogen were analysed by standard methods18.

Feed intake and feed efficiency: Dry Matter Intake (DMI) was calculated as the DM of the diet/100*the total diet intake and Organic Matter Intake (OMI) was calculated as the OM of the diet/100*the total diet intake. Feed intake was based on the metabolic body weight and calculated as the DMI or OMI/BW0.75*1000 and feed efficiency was calculated as ADG/DM intake *100%.

Table 1: Ingredients and nutrient compositions of the experimental diets (DM basis)

Table 2: Feed intake, daily weight gain, methane gas production and nitrogen retention of Bali cattle fed experimental diets
Description: Different letters (a,b,c) in the same row indicate significant differences between treatments (p<0.05)

Methane determination: Methane production was measured using the methane emission estimation method (Shibata’s equation), as follows:

where, DMI = Dry Matter Intake (kg day–1)

Nitrogen retention: Nitrogen retention was calculated as the nitrogen intake minus the nitrogen contained in the faeces and urine19. This value can be used to determine whether the nitrogen balance is positive or negative.

Statistical analysis: Statistical analysis was performed using SAS program20 and differences among the treatments were evaluated by one-way (ANOVA). Duncan’s Multiple Range Test (DMRT) was used for comparison of means of treatments.

RESULTS AND DISCUSSION

The DMI, OMI, ADG, feed efficiency, methane production and nitrogen retention of the experimental rations with and without DFM and VCO supplementations are summarized in Table 2.

Table 2 shows that SC+VCO supplementation significantly (p<0.05) reduced DMI and OMI, while increasing ADG. Virgin coconut oil contains lauric acid, which has maximum digestibility, so this component can be digested more rapidly than other types of fat and quickly used as a source of energy21,22. Furthermore, the energy that it provided to the cattle decreased feed intake. Diet supplementation with DFM produced a beneficial microorganismal balance that reduced nutrient degradation in the rumen23. Therefore, DFM supplementation of beef cattle diets could improve growth performance, milk and meat production as has been shown in many studies24,25.

Supplementation with SC+VCO could significantly (p<0.05) increase ADG. Increased ADG by a probiotic has been shown to increase DM and protein intake as well as nitrogen retention26,27. Saccharomyces sp., could stimulate the growth of rumen bacteria, especially cellulolytic bacteria that influence feed intake and digestibility and thus influence the ADG28. In this study, direct fed microbials, benefitted ruminants and influenced meat production29 and daily gain (ADG) was higher in calves receiving a combination of S. cerevisiae and enzyme30. Supplementation with the DFMSC alone and combine with B. amyloliquefaciens (treatment B and C) had the same result on ADG. Qiao et al.31 reported that B. licheniformis supplementation increased dry matter intake and milk protein percentages. Furthermore, the DFMSC could modify the rumen ecosystem to better degrade fibre32.

Table 2 shows that supplementation with SC+VCO significantly (p<0.05) decreased methane gas production indicating that there was a synergistic effect when the DFM SC was combined with VCO. VCO has MCFA that decrease methane production but SC also inhibits methane production. This is supported by Ipharraguerre and Clark33, who found that diet supplementation with coconut oil could decrease methanogens. Furthermore, coconut oil can reduce methane production by inhibiting Archaea methanogens in the rumen14. Supplementation with VCO as source of MCFA could also increase feed energy and thus increase ruminant productivity34 and supplement MCFA could be directly absorbed by the rumen walls and potentially inhibit methanogenesis35,36. Furthermore, SC has the potential to reduce methane production because it can stimulate acetogens to compete with methanogen bacteria37.

Table 2 shows that the highest mean feed efficiency value occurred under treatment D so SC+VCO supplementation could significantly (p<0.05) increase feed efficiency. When the feed efficiency value was higher, the feed were more efficient and higher quality, which reduced DM intake but increased the ADG under treatment D. Tillman et al.38 stated that feed efficiency value depends on the total DM intake which could increase ADG. Furthermore, lactic acid production and DFM utilization in the rumen are related to feed efficiency and ruminant health8,39. Combining S. cerevisiae with enzymes could increase the feed efficiency of calves30 and DFM supplementation of beef cattle feed could improve feed efficiency40,41.

Supplementation with SC+VCO significantly (p<0.05) decreased nitrogen retention but although N retention was low under treatment D, there was a significant (p<0.05) increase in ADG compared with treatments A, B and C. Indicating an increase in microbial protein synthesis. The retention of N was low under treatment D because the feed intake was low and the protein intake indicated that little was being assimilated by the body. Chuzaemi42 reported that the factors that affecting the nitrogen balance include nitrogen intake and nitrogen excretion through the faeces and urine. The protein in ruminants is derived from microbial proteins and the feed43. Sutardi44 explained that not all the nitrogen that is consumed can be retained but is partially lost through faeces and urine. This experiment produced a positive N retention value indicating that the amount of N consumed was larger than the amount released45.

CONCLUSION

Supplementation with the DFM SC alone, the combination of the DFMs SC+BA and the combination of SC+VCO could decrease nutrient intake but also increase ADG and feed efficiency as well as decrease methane production and nitrogen retention. Supplementation with SC+VCO resulted in the best improvement in Bali cattle growth performance and reduced methane gas production by up to 20.63%.

SIGNIFICANCE STATEMENT

This study demonstrates that supplementation with direct-fed microbials and virgin coconut oil can increase the growth performance and reduce the methane gas production and feed efficiency of Bali beef cattle. This study will enable researchers to further investigate the critical area of methane production by Bali cattle fed an ammoniated palm frond-based diet, which was previously unexplored. Thus, a new hypothesis regarding the effects of the supplement combination of DFM and VCO may be developed.

ACKNOWLEDGEMENTS

This study was supported by a National Strategic Research Grant from the Directorate General for Higher Education of the Department of National Education Republic of Indonesia through PMDSU grant program 2016.

REFERENCES
1:  Directorate General of Plantations, 2015. Statistic of plantation book. Directorate General of Plantations, Jakarta, Indonesia.

2:  Zain, M., T. Sutardi, Suryahadi and N. Ramli, 2008. Effect of defaunation and supplementation methionine hydroxy analogue and branched chain amino acid in growing sheep diet based on palm press fiber ammoniated. Pak. J. Nutr., 7: 813-816.
CrossRef  |  Direct Link  |  

3:  Klopfenstein, T., 1978. Chemical treatment of crop residues. J. Anim. Sci., 46: 841-848.
CrossRef  |  Direct Link  |  

4:  Dohme, F., A. Machmuller, B.L. Estermann, P. Pfister, A. Wasserfallen and M. Kreuzer, 1999. The role of the rumen ciliate protozoa for methane suppression caused by coconut oil. Lett. Applied Microbiol., 29: 187-192.
CrossRef  |  Direct Link  |  

5:  Mutsvangwa, T., I.E. Edwards, J.H. Topps and G.F.M. Paterson, 1992. The effect of dietary inclusion of yeast culture (Yea-Sacc) on patterns of rumen fermentation, food intake and growth of intensively fed bulls. Anim. Prod., 55: 35-40.
CrossRef  |  Direct Link  |  

6:  Brashears, M.M., A. Amezquita and D. Jaroni, 2005. Lactic acid bacteria and their uses in animal feeding to improve food safety. Adv. Food Nutr. Res., 50: 1-31.
CrossRef  |  Direct Link  |  

7:  Lynch, H.A. and S.A. Martin, 2002. Effects of Saccharomyces cerevisiae culture and Saccharomyces cerevisiae live cells on in vitro mixed ruminal microorganism fermentation. J. Dairy Sci., 85: 2603-2608.
CrossRef  |  Direct Link  |  

8:  Chaucheyras, F., G. Fonty, G. Bertin, J.M. Salmon and P. Gouet, 1996. Effects of a strain of Saccharomyces cerevisiae (Levucell SC1), a microbial additive for ruminants, on lactate metabolism in vitro. Can. J. Microbiol., 42: 927-933.
PubMed  |  Direct Link  |  

9:  Lascano, G.J., A.J. Heinrichs and J.M. Tricarico, 2012. Substitution of starch by soluble fiber and Saccharomyces cerevisiae dose response on nutrient digestion and blood metabolites for precision-fed dairy heifers. J. Dairy Sci., 95: 3298-3309.
CrossRef  |  Direct Link  |  

10:  Herawaty, R., N. Jamarun, M. Zain, Arnim and R.W.S. Ningrat, 2013. Effect of supplementation Saccharomyces cerevisiae and Leucaena leucocephala on low quality roughage feed in beef cattle diet. Pak. J. Nutr., 12: 182-184.
CrossRef  |  Direct Link  |  

11:  Wizna, H.A., Y. Rizal, A. Dharma and I.P. Kompiang, 2007. Selection and identification of cellulase-producing bacteria isolated from the litter of mountain and swampy forest. Microbiol. Indonesia, 1: 135-139.
Direct Link  |  

12:  Suryani, H., M. Zain, R.W.S. Ningrat and N. Jamarun, 2016. Supplementation of direct fed microbial (DFM) on in vitro fermentability and degradability of ammoniated palm frond. Pak. J. Nutr., 15: 90-95.
CrossRef  |  Direct Link  |  

13:  Miller, T.L., M.J. Wolin, H.X. Zhao and M.P. Bryant, 1986. Characteristics of methanogens isolated from bovine rumen. Applied Environ. Microbiol., 51: 201-202.
Direct Link  |  

14:  Machmuller, A., 2006. Medium-chain fatty acids and their potential to reduce methanogenesis in domestic ruminants. Agric. Ecosyst. Environ., 112: 107-114.
CrossRef  |  Direct Link  |  

15:  Dohme, F., A. Machmuller, A. Wasserfallen and M. Kreuzer, 2001. Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets. Lett. Applied Microbiol., 32: 47-51.
CrossRef  |  PubMed  |  Direct Link  |  

16:  Marina, A.M., Y.B. Che Man, S.A.H. Nazimah and I. Amin, 2009. Chemical properties of virgin coconut oil. J. Am. Oil Chem. Soc., 86: 301-307.
CrossRef  |  Direct Link  |  

17:  Yang, S.Y., R.W.S. Ningrat, J.S. Eun and B.R. Min, 2016. Effects of supplemental virgin coconut oil and condensed tannin extract from pine bark in lactation dairy diets on ruminal fermentation in a dual-flow continuous culture system. J. Adv. Dairy Res., Vol. 4. 10.4172/2329-888X.1000160

18:  AOAC., 1990. Official Methods of Analysis of the AOAC. 15th Edn., Association of Official Analytical Chemists, Arlington, VA., USA.

19:  Tillman, A.D., H. Hartadi, S. Reksohadiprodjo, S. Prawirokusumo and S. Lebdosoekojo, 1991. Basic of Livestock's Feed. Gadjah Mada University Press, Yogyakarta, Indonesia.

20:  SAS., 1985. SAS User's Guide: Statistics. Version 5, SAS Institute Inc., Cary, NC., USA.

21:  Oopik, V., S. Timpmann, L. Medijainen and H. Lemberg, 2001. Effects of daily medium-chain triglyceride ingestion on energy metabolism and endurance performance capacity in well-trained runners. Nutr. Res., 21: 1125-1135.
CrossRef  |  Direct Link  |  

22:  Nevin, K.G. and T. Rajamohan, 2006. Virgin coconut oil supplemented diet increases the antioxidant status in rats. Food Chem., 99: 260-266.
CrossRef  |  Direct Link  |  

23:  Williams, P.E.V. and C.J. Newbold, 1990. Rumen Probiosis: The Effects of Novel Microorganisms on Rumen Fermentation and Ruminant Productivity. In: Recent Advances in Animal Nutrition, Haresign, W. and D.J.A. Cole (Eds.). Buttenvorths, London, pp: 211.

24:  Ghorbani, G.R., D.P. Morgavi, K.A. Beauchemin and J.A.Z. Leedle, 2002. Effects of bacterial direct-fed microbials on ruminal fermentation, blood variables and the microbial populations of feedlot cattle. J. Anim. Sci., 80: 1977-1985.
PubMed  |  Direct Link  |  

25:  Nocek, J.E., W.P. Kautz, J.A.Z. Leedle and J.G. Allman, 2002. Ruminal supplementation of direct-fed microbials on diurnal pH variation and in situ digestion in dairy cattle. J. Dairy Sci., 85: 429-433.
CrossRef  |  Direct Link  |  

26:  Ngadiyono, N. and E. Baliarti, 2001. [Growth rate and carcass production of ongole cattle with starbio probiotic supplementation in cattle feed]. Media Peternakan: J. Anim. Sci. Technol., 24: 63-67, (In Indonesian).
Direct Link  |  

27:  Hau, D.K., N.G.F. Katipana, J. Nulik, A. Pohan, O.T. Lailogo and C. Liem, 2004. Effect of probiotic on nitrogen retention, energy and growth of Bali cattle. Proceedings of the National Seminar on Technology and Veterinary Science, August 4-5, 2004, Bogor, Indonesia, pp: 91-96.

28:  Callaway, E.S. and S.A. Martin, 1997. Effects of a Saccharomyces cerevisiae culture on ruminal bacteria that utilize lactate and digest cellulose. J. Dairy Sci., 80: 2035-2044.
CrossRef  |  PubMed  |  Direct Link  |  

29:  Wallace, R.J., 1994. Ruminal microbiology, biotechnology and ruminant nutrition: Progress and problems. J. Anim. Sci., 72: 2992-3003.
PubMed  |  Direct Link  |  

30:  Malik, R. and S. Bandla, 2010. Effect of source and dose of probiotics and Exogenous Fibrolytic Enzymes (EFE) on intake, feed efficiency and growth of male buffalo (Bubalus bubalis) calves. Trop. Anim. Health Prod., 42: 1263-1269.
CrossRef  |  Direct Link  |  

31:  Qiao, G.H., A.S. Shan, N. Ma, Q.Q. Ma and Z.W. Sun, 2010. Effect of supplemental Bacillus cultures on rumen fermentation and milk yield in Chinese holstein cows. J. Anim. Physiol. Anim. Nutr., 94: 429-436.
CrossRef  |  Direct Link  |  

32:  Zain, M., J. Rahman, Khasrad and Erpomen, 2015. In vitro fermentation characteristics of palm oil byproducts which is supplemented with growth factor rumen microbes. Pak. J. Nutr., 14: 625-628.
CrossRef  |  Direct Link  |  

33:  Ipharraguerre, I.R. and J.H. Clark, 2003. Usefulness of ionophores for lactating dairy cows: A review. Anim. Feed Sci. Technol., 106: 39-57.
CrossRef  |  Direct Link  |  

34:  Fife, B., 2001. Coconut Oil: A Low-Calorie Fat. In: The Healing Miracles of Coconut Oil, Fife, B. (Ed.). Players Press, UK., ISBN-13: 9780941599511.

35:  Bauman, D.E., A.L. Lock, B.A. Corl, A.M. Salter and P.M. Parodi, 2005. Milk Fatty Acids and Human Health: Potential Role of Conjugated Linoleic Acid and Trans Fatty Acids. In: Ruminant Physiology: Digestion Metabolism and Impact of Nutrition on Gene Expression Immunology and Stress, Sejrsen, K., T. Hvelplund and M.O. Nielsen (Eds.). Wageningen Academic Publishers, Wageningen, pp: 529-561.

36:  Soliva, C.R., I.K. Hindrichsen, L. Meile, M. Kreuzer and A. Machmuller, 2003. Effects of mixtures of lauric and myristic acid on rumen methanogens and methanogenesis in vitro. Lett. Applied Microbiol., 37: 35-39.
CrossRef  |  PubMed  |  Direct Link  |  

37:  Chaucheyras, F., G. Fonty, G. Bertin and P. Gouet, 1995. In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in association with an archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae. Applied Environ. Microbiol., 61: 3466-3467.
Direct Link  |  

38:  Tillman, A.D., H. Hartadi, S. Prawirokusumo, S. Reksohadiprodjo and S. Lebdosoekojo, 1998. Basic of Livestock's Feed. 6th Edn., Gadjah Mada University Press, Yogyakarta, Indonesia.

39:  Zain, M., N. Jamarun, A. Arnim, R.W.S. Ningrat and R. Herawati, 2011. Effect of yeast (Saccharomyces cerevisiae) on fermentability, microbial population and digestibility of low quality roughage in vitro. Arch. Zootech., 14: 51-58.
Direct Link  |  

40:  Krehbiel, C.R., S.R. Rust, G. Zhang and S.E. Gilliland, 2003. Bacterial direct-fed Microbials in ruminant diets: Performance response and mode of action. J. Anim. Sci., 81: E120-E132.
Direct Link  |  

41:  Stein, D.R., D.T. Allen, E.B. Perry, J.C. Bruner and K.W. Gates et al., 2006. Effects of feeding propionibacteria to dairy cows on milk yield, milk components and reproduction. J. Dairy Sci., 89: 111-125.
CrossRef  |  PubMed  |  Direct Link  |  

42:  Chuzaemi, S., 1986. Effect of urea ammoniation on chemical composition and nutrition value of rice straw to Ongle cattle. M.Sc. Thesis, Faculty of Animal Science, Gadjah Mada University, Yogyakarta.

43:  Soebarinoto, S. Chuzaemi and Mashudi, 1991. Science of ruminant nutrition. Department of Animal Nutrition, Brawijaya University, Malang, Indonesia.

44:  Sutardi, T., 2006. Basic of livestock's nutrition. Department of Animal Nutrition, Faculty of Animal Science, Bogor Agriculture University, Bogor.

45:  McDonald, P., R.A. Edwards and J.F. Greenhalgh, 1988. Animal Nutrition. Longman Scientific and Technical, Essex, UK., ISBN-13: 9780582409033, Pages: 543.

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