Subscribe Now Subscribe Today
Research Article
 

An Aqueous Extract of Black Rice Bran from the Cibeusi Variety Prevents Anemia and Hypertriglyceridemia in Rats



Enny Purwati Nurlaili, Mary Astuti, Yustinus Marsono and Sri Naruki
 
ABSTRACT

Rice is a leading staple food in Southeast Asia and it is typically milled before consumption. Black rice found in Indonesia is classified as Oryza sativa L. The color in the grain is caused by anthocyanin pigments that give the hulled rice a dark purple color. We found that extracts from black rice bran have high levels of iron and anthocyanins. The objective of this study was to evaluate the potential of an aqueous extract of black rice bran to prevent anemia and hypertriglyceridemia in vivo. Anemia and hypertriglyceridemia were induced in twenty-eight three-week old male albino Wistar rats (Rattus norvegicus); the rats were divided into a control (C) group or 3 treatment groups of bran (B), bran extract (E), or extraction residue (R). The results showed that differences among the groups based on feed consumption, Hemoglobin Regeneration Efficiency (HRE) and the erythrocyte morphology of rats were not significant. Black rice bran aqueous extract prevented anemia and hypertriglyceridemia by increasing the hemoglobin Hb level from 7.21 to 12.96 g/dl and by reducing triglycerides from 179.29 to 56.55 mg/dl.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Enny Purwati Nurlaili, Mary Astuti, Yustinus Marsono and Sri Naruki, 2016. An Aqueous Extract of Black Rice Bran from the Cibeusi Variety Prevents Anemia and Hypertriglyceridemia in Rats. Pakistan Journal of Nutrition, 15: 837-845.

DOI: 10.3923/pjn.2016.837.845

URL: https://scialert.net/abstract/?doi=pjn.2016.837.845

REFERENCES
Allen, L., B. de Benoist, O. Dary and R. Hurrell, 2006. Guidelines on Food Fortification with Micronutrients. World Health Organization, Rome, ISBN: 9789241594011, Pages: 370.

Almatsier, S., 2001. Prinsip Dasar Ilmu Gizi. Gramedia Pustaka Utama, Jakarta, Indonesia.

Brownlie, T., V. Utermohlen, P.S. Hinton and J.D. Haas, 2004. Tissue iron deficiency without anemia impairs adaptation in endurance capacity after aerobic training in previously untrained women. Am. J. Clin. Nutr., 73: 437-443.
Direct Link  |  

Choi, J.W., S.K. Kim and S.H. Pai, 2001. Changes in serum lipid concentrations during iron depletion and after iron supplementation. Ann. Clin. Laboratory Sci., 31: 151-156.
Direct Link  |  

Fields, M. and C.G. Lewis, 1999. Cholesterol-lowering nature of unsaturated fat in rats may be due to its inability to increase hepatic iron. Metabol. Clin. Exp., 48: 200-204.
CrossRef  |  Direct Link  |  

Ghiselli, A., M. Nardini, A. Baldi and C. Scaccini, 1998. Antioxidant activity of different phenolic fractions separated from an Italian red wine. J. Agric. Food Chem., 46: 361-367.
CrossRef  |  Direct Link  |  

Gregorio, G.B., D. Senadhira, T. Htut and R.D. Graham, 1999. Improving iron and zinc value of rice for human nutrition. Agriculture et Developpement, 23: 77-81.
Direct Link  |  

Haas, J.D. and T. Brownlie, 2001. Iron deficiency and reduced work capacity: A critical review of the research to determine a causal relationship. J. Nutr., 131: 676S-690S.
Direct Link  |  

Hernandez, M., V. Sousa, A. Moreno, S. Villapando and M. Lopez-Alarcon, 2003. Iron bioavailability and utilization in rats are lower from lime-treated corn flour than from wheat flour when they are fortified with different sources of iron. J. Nutr., 133: 154-159.
PubMed  |  Direct Link  |  

Kaneda, I., F. Kubo and H. Sakurai, 2007. Relationship between trace metal concentration and antioxidative activity of ancient rice bran (red and black rice) and a present-day rice bran (Koshihikari). J. Trace Element Med. Biol., 21: 45-51.
CrossRef  |  Direct Link  |  

Kaneda, I., H. Yasui, Y. Adachi, J. Takada and H. Sakurai, 2005. Determination of trace element concentrations in ancient rices (red and black rices) and a present-day rice (Koshihikari): Relationship among the trace element concentrations, species, harvest site and rice parts. Biomed. Res. Trace Elements, 16: 241-249.
CrossRef  |  Direct Link  |  

Kim, J., X. Jia, P.D. Buckett, S. Liu, C.H. Lee and M. Wessling-Resnick, 2013. Iron loading impairs lipoprotein lipase activity and promotes hypertriglyceridemia. FASEB J., 27: 1657-1663.
CrossRef  |  Direct Link  |  

Kong, S. and J. Lee, 2010. Antioxidants in milling fractions of black rice cultivars. Food Chem., 120: 278-281.
CrossRef  |  Direct Link  |  

Meng, F., Y. Wei and X. Yang, 2005. Iron content and bioavailability in rice. J. Trace Elem. Med. Biol., 18: 333-338.
CrossRef  |  PubMed  |  Direct Link  |  

MoH., 2013. Basic health research 2013. The Ministry of Health, Agency for Health Research and Development, Jakarta, Indonesia.

Mpiana, P.T., K.N. Ngbolua, M.T. Bokota, T.K. Kasonga, E.K. Atibu, D.S. Tshibangu and V. Mudogo, 2010. In vitro effects of anthocyanin extracts from Justicia secunda VAHL on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfus., 8: 248-254.
CrossRef  |  PubMed  |  Direct Link  |  

Ono, K., N. Sugihara, Y. Hirose and K. Katagiri, 2003. An examination of optimal extraction solvents for anthocyanin pigments from black rice produced in Gifu. J. Gifu City Women's College, 52: 135-138, (In Japanese).
Direct Link  |  

Provan, D., 1999. Mechanisms and management of iron deficiency anaemia. Br. J. Haematol., 105: 19-26.
PubMed  |  Direct Link  |  

Reeves, P.G., F.H. Nielsen and G.C. Fahey Jr., 1993. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr., 123: 1939-1951.
CrossRef  |  PubMed  |  Direct Link  |  

Stoltzfus, R.J., J.D. Kvalsvig, H.M. Chwaya, A. Montresor and M. Albonico, 2002. Effects of iron supplementation and anthelmintic treatment on motor and language development of preschool children in Zanzibar: Double blind, placebo controlled study. Br. Med. J., 323: 1389-1393.
CrossRef  |  Direct Link  |  

Tedesco, I., G.L. Russo, F. Nazzaro, M. Russo and R. Palumbo, 2001. Antioxidant effect of red wine anthocyanins in normal and catalase-inactive human erythrocytes. J. Nutr. Biochem., 12: 505-511.
CrossRef  |  Direct Link  |  

Theil, E.C., 2004. Iron, ferritin and nutrition. Annu. Rev. Nutr., 24: 327-343.
CrossRef  |  Direct Link  |  

WHO., 2001. Iron Deficiency Anaemia Assessment Prevention and Control. A Guide for Programme Managers. World Health Organization, Geneva, Switzerland.

Wu, A.C., L. Lesperance and H. Bernstein, 2002. Screening for iron deficiency. Pediatr. Rev., 23: 171-177.
Direct Link  |  

Yamagishi, H., H. Okazaki, M. Shimizu, T. Izawa and T. Komabayashi, 2000. Relationships among serum triacylglycerol, fat pad weight and lipolysis in iron-deficient rats. J. Nutr. Biochem., 11: 455-460.
CrossRef  |  Direct Link  |  

©  2019 Science Alert. All Rights Reserved