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Research Article
 

Comparison Study on the Extraction of Gelatin from Nila Fish (Oreochromis nilotichus) Skin Using Acetic Acid and Citric Acid



Suryanti , Retno Indrati, Hari Eko Irianto and Djagal Wiseso Marseno
 
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ABSTRACT

Methods of gelatin extraction from nila fish (Oreochromis nilotichus) skin were studied using acid treatment (acetic acid or citric acid) at various concentrations (0, 0.05, 0.10, 0.15, 0.20 and 0.25 M) and soaking times (0, 1, 2, 3, 4 and 5 h). Gelatin extracted from nila skin treated with 0.10 M acetic acid for 2 h produced the highest yield (25.33%) with a gel strength of 346.16 g bloom and a molecule size distribution of 38-241 kDa. Gelatin extracted from the skin treated with 0.05 M citric acid for 1 h resulted in a yield of 21.09% with a gel strength of 134.52 g bloom and a molecule size distribution of 23-145 kDa. Gelatin extracted by both acids had similar infra-red absorbance spectra, ranging from 1640 to 1239 cm-1. However, gelatin from nila skin treated with 0.10 M acetic acid for 2 h produced gelatin with a more homogenous molecular microstructure than nila skin treated with 0.05 M citric acid for 1 h.

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  How to cite this article:

Suryanti , Retno Indrati, Hari Eko Irianto and Djagal Wiseso Marseno, 2016. Comparison Study on the Extraction of Gelatin from Nila Fish (Oreochromis nilotichus) Skin Using Acetic Acid and Citric Acid. Pakistan Journal of Nutrition, 15: 777-783.

DOI: 10.3923/pjn.2016.777.783

URL: https://scialert.net/abstract/?doi=pjn.2016.777.783

REFERENCES

1:  Ahmad, M. and S. Benjakul, 2011. Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time. Food Hydrocolloids, 25: 381-388.
CrossRef  |  Direct Link  |  

2:  Amiza, M.A. and D.S. Aishah, 2011. Effect of drying and freezing of Cobia (Rachycentron canadum) skin on its gelatin properties. Int. Food Res. J., 18: 159-166.
Direct Link  |  

3:  Anonymous, 2012. Gelatin Handbook. Gelatin Manufacture Institute of America, Amerika

4:  AOAC., 1995. Official Methods of Analysis. 15th Edn., Association of Official Analytical Chemists, Washington, DC., USA

5:  Ayudiarti, D.L., Tazwir, Suryanti and R. Peranginangin, 2007. Effect of fish gelatin concentration as binder on the quality and acceptability of syrup. J. Fish. Sci., 9: 134-141.
Direct Link  |  

6:  Cebi, N., M.Z. Durak, O.S. Toker, O. Sagdic and M. Arici, 2016. An evaluation of Fourier transforms infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins. Food Chem., 190: 1109-1115.
CrossRef  |  Direct Link  |  

7:  Hashim, D.M., Y.B.C. Man, R. Norakasha, M. Shuhaimi, Y. Salmah and Z.A. Syahariza, 2010. Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins. Food Chem., 118: 856-860.
CrossRef  |  Direct Link  |  

8:  Jackson, M. and H.H. Mantsch, 1995. The use and misuse of FTIR spectroscopy in the determination of protein structure. Crit. Rev. Biochem. Mol. Biol., 30: 95-120.
CrossRef  |  PubMed  |  Direct Link  |  

9:  Jamilah, B., K.W. Tan, M.R. Umi Hartina and A. Azizah, 2011. Gelatins from three cultured freshwater fish skins obtained by liming process. Food Hydrocolloids, 25: 1256-1260.
CrossRef  |  Direct Link  |  

10:  Kent, M.J., N.D. Light and A.J. Bailey, 1985. Evidence for glucose-mediated covalent cross-linking of collagen after glycosylation in vitro. Biochem. J., 225: 745-752.
PubMed  |  Direct Link  |  

11:  Muyonga, J.H., C.G.B. Cole and K.G. Duodu, 2004. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin. Food Hydrocolloids, 18: 581-592.
CrossRef  |  Direct Link  |  

12:  Muyonga, J.H., C.G.B. Cole and K.G. Duodu, 2004. Fourier Transform Infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem., 86: 325-332.
CrossRef  |  Direct Link  |  

13:  Niu, L., X. Zhou, C. Yuan, Y. Bai, K. Lai, F. Yang and Y. Huang, 2013. Characterization of tilapia (Oreochromis niloticus) skin gelatin extracted with alkaline and different acid pretreatments. Food Hydrocolloids, 33: 336-341.
CrossRef  |  Direct Link  |  

14:  Peranginangin, R., Mulyasari, A. Sari and Tazwir, 2005. Characteristic of quality Gelatin from Patin fish (Pangsius sp) bone by acid extracted. J. Post-Harvest Biotechnol. Mar. Fish., 4: 15-24.

15:  Peranginangin, R., Tazwir, Suryanti and D.A. Lestari, 2006. Optimization research of waste utilizationof fish bone and skin. Technical reports. Research Center for Marine and Fisheries Product Processing and Socio Economic, Jakarta.

16:  Pranoto, Y., D.W. Marseno and H. Rahmawati, 2011. Characteristics of gelatins extracted from fresh and sun-dried seawater fish skins in Indonesia. Int. Food Res. J., 18: 1335-1341.
Direct Link  |  

17:  Schrieber, R. and H. Garies, 2007. Gelatin Handbook. WILEY-VCH Verlag GmbH and Co., USA

18:  See, S.F., P.K. Hong, K.L. Ng, W.M. Wan Aida and A.S. Babji, 2010. Physicochemical properties of gelatins extracted from skins of different freshwater fish species. Int. Food Res. J., 17: 809-816.
Direct Link  |  

19:  Setiyowati, E., 2014. Characterization of gelatinof nila (Oreochromis niloticus) skin and the application injellysweets. Master's Thesis, Gajah Mada University, Yogyakarta.

20:  Sinthusamran, S., S. Benjakul and H. Kishimura, 2014. Characteristics and gel properties of gelatin from skin of seabass (Lates calcarifer) as influenced by extraction conditions. Food Chem., 152: 276-284.
CrossRef  |  Direct Link  |  

21:  SNI., 1995. Quality and Test methods of Gelatin. National Standardization Agency, Jakarta, Indonesia

22:  Suryanti, R. Peranginangin and S. Hadi, 2006. Extraction of Gelatin from fish bone red kakap (Lutjanus sp.). J. Postharvest Biotechnol. Mar. Fish., 4: 27-34.

23:  Ward, A.G. and A. Coutrs, 1977. Technology of Gelatin. CRC Press, New York

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