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Research Article

Effect of Storage of Therapeutic Product on LAB Bacterial Counts and Acidity

Shatha Khalil, Abdul- Ahad, Ali Mohamed Husein and Hala Abdulmunam Yaseen
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This investigation carried out to utilize whey and rice in preparation of a therapeutic product. The raw materials were mixed at a ratio of 1:10 rice to whey (w/w). Five treatments were prepared by fermentation with either Lb. acidophilus and Lb. casei, or mixes of both. The treatments were: T1 (Lb. acidophilus), T2 (Lb. casei), T3, T4, T5 which were mixes of (Lb. acidophilus+Lb. casei) starters with ratios of 1:1, 1:2, 2:1, respectively. The best fermentation period was two days as highest therapeutic bacterial counts resulted. The treatments were divided into two groups; first group stored at -18°C for four months, the second group dried at 45°C, then stored at 25°C for three months. The frozen treatments revealed significant differences (p<0.05) in titrable acidity which were 0.43-0.85% for T2, T3, respectively. The pH values were (3.63-4.72) for T4, T2, respectively. LAB counts dropped for all treatments during frozen storage. The drop was between 19.14-60.05% for T1 and T4, respectively. The titrable acidity for dried treatments was 0.33-0.66% for T2, T1, respectively. Then increased to 0.46-0.75% after three months of storage for T2, T5, respectively. Highest pH value of 4.5 obtained for T2. There was a slight drop in pH value for all treatments after 3 months of storage. LAB counts dropped for all treatments after drying. The highest number of 5.48 × 1010 cfu/gm was for T4. LAB counts were acceptable to impart therapeutic character to the product.

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  How to cite this article:

Shatha Khalil, Abdul- Ahad, Ali Mohamed Husein and Hala Abdulmunam Yaseen, 2015. Effect of Storage of Therapeutic Product on LAB Bacterial Counts and Acidity. Pakistan Journal of Nutrition, 14: 366-369.

DOI: 10.3923/pjn.2015.366.369


1:  AOAC., 2005. Association of Official Analytical Chemists of Official Methods of Analysis. 18th Edn., AOAC., Maryland, Washington, DC., USA.

2:  Heller, K.J., 2001. Probiotic bacteria in fermented foods: Product characteristics and starter organisms 1'2'3. Am. J. Clin. Nutr., 73: 374S-379S.
PubMed  |  Direct Link  |  

3:  Huebner, J., R.L. Wehling, A. Parkhurst and R.W. Hutkins, 2008. Effect of processing conditions on the prebiotic activity of commercial prebiotics. Int. Dairy J., 18: 287-293.
CrossRef  |  Direct Link  |  

4:  Lankaputhra, W.E.V. and N.P. Shah, 1995. Survival of Lactobacillus acidophilus and Bifidobacterium spp in the presence of acid and bile salts. Cult. Dairy Prod. J., 30: 2-7.
Direct Link  |  

5:  Sanders, M.E., 2000. Considerations for use of probiotic bacteria to modulate human health. J. Nutr., 130: 384S-390S.
Direct Link  |  

6:  Senok, A.C., 2009. Probiotics in the Arabian Gulf Region. Food Nutr. Res., Vol. 53. 10.3402/fnr.v53i0.1842.

7:  Shah, N.P., 2000. Probiotic bacteria: Selective enumeration and survival in dairy foods. J. Dairy Sci., 83: 894-907.
PubMed  |  Direct Link  |  

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