Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 

Chemical Characteristics of Collagen Extract from Scapula of Bali Cattle (Os scapula) Produced Using Different Extractant



Muhammad Irfan Said, Effendi Abustam, Abd Wahid Wahab, Sartini and Amriana Hifizah
 
ABSTRACT

Collagen is a hydrocolloid products of animal protein resulted in partial hydrolysis. Cow bones abattoir by-product is a product that is rich in collagen proteins, thereby it’s potentially as a source of raw materials. The protein collagen is now widely used as a dietary supplement. This study aimed to evaluate the chemical properties of the extract collagen product from os scapula of Bali cattle through the application of some types of extractant in the production process. Laboratory experimental method was used in this study. A total of four types of extractant was applied, namely: (1) distilled water, (2) ethanol 60% (v/v), (3) CH3COOH 0.5 M and (4) Ca(OH)2 0.5 M. The pattern of Complete Randomized Design (RAL) of Undirectional pattern and the analysis of variance was used as the study design and data analysis, while Duncan's Multiple Range Test (DMRT) was used as a further test if it was significant. The results showed that the use of different extractant extracts on the collagen production processes using raw materials from os scapula of Bali cattle affected (p<0.05) the value of the yield, moisture content, protein content and calcium levels, but there was no significant effect (p>0.05) on lipid levels and phosphorus levels. Extract collagen from os scapula of Bali cattle produced using four types of extractants have functional properties identical functional groups based on the presence of CO, C-O, OH, CH and N-H bond.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Muhammad Irfan Said, Effendi Abustam, Abd Wahid Wahab, Sartini and Amriana Hifizah, 2015. Chemical Characteristics of Collagen Extract from Scapula of Bali Cattle (Os scapula) Produced Using Different Extractant. Pakistan Journal of Nutrition, 14: 174-179.

DOI: 10.3923/pjn.2015.174.179

URL: https://scialert.net/abstract/?doi=pjn.2015.174.179

REFERENCES
AOAC., 2005. Official Methods of Analysis of AOAC International. 18th Edn., AOAC International, Gaithersburg, MD., USA., ISBN-13: 978-0935584752.

English, J. and H. Cass, 2011. The collagen connection. Nutr. Rev.

Gimenez, B., M.C. Gomez-Guillen and P. Montero, 2005. The role of salt washing of fish skins in chemical and rheological properties of gelatin extracted. Food Hydrocolloids, 19: 951-957.
CrossRef  |  Direct Link  |  

Gimenez, B., M.C. Gomez-Guillen and P. Montero, 2005. Storage of dried fish skins on quality characteristics of extracted gelatin. Food Hydrocolloids, 19: 958-963.
CrossRef  |  Direct Link  |  

Karim, A.A. and R. Bhat, 2008. Gelatin alternatives for the food industry: Recent developments, challenges and prospects. Trends Food Sci. Technol., 19: 644-656.
CrossRef  |  Direct Link  |  

Kasankala, L.M., Y. Xue, Y. Weilong, S.D. Hong and Q. He, 2007. Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology. Bioresour. Technol., 98: 3338-3343.
CrossRef  |  Direct Link  |  

Muyonga, J.H., C.G.B. Cole and K.G. Duodu, 2004. Fourier Transform Infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem., 86: 325-332.
CrossRef  |  Direct Link  |  

Ockerman, H.W. and C.L. Hansen, 2000. Animal By Product Processing and Utilization. CRC Press, USA.

Rogart, J.N., H.J. Barrach and C.O. Chichester, 1999. Articular collagen degradation in the Hulth-Telhag model of osteoarthritis. Osteoarthritis Cartilage., 7: 539-547.
CrossRef  |  Direct Link  |  

Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd Edn., McGraw Hill Book Co., New York, USA., ISBN-13: 978-0070609259, Pages: 481.

Tian, Y., Z. Peng, D. Gorton, Y. Xiao and N. Ketheesan, 2011. Immunohistochemical analysis of structural changes in collagen for the assessment of osteoarthritis. Proc. Inst. Mech. Eng. Part H: J. Eng. Med., 225: 680-687.
CrossRef  |  Direct Link  |  

Wang, L., B. Yang, X. Du, Y. Yang and J. Liu, 2008. Optimization of conditions for extraction of acid-soluble collagen from grass carp (Ctenopharyngodon idella) by response surface methodology. Innov. Food Sci. Emerg. Technol., 9: 604-607.
CrossRef  |  Direct Link  |  

Zeugolis, D.I., S.T. Khew, E.S.Y. Yew, A.K. Ekaputra and Y.W. Tong et al., 2008. Electro-spinning of pure collagen nano-fibres-just an expensive way to make gelatin? Biomaterials, 29: 2293-2305.
CrossRef  |  Direct Link  |  

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract