Haoua Sabo
Department of Chemistry, Faculty of Sciences and Technology, Abdou Moumouni University, PO Box: 10 662, Niamey, Niger
Hassimi Sadou
Department of Chemistry, Faculty of Sciences and Technology, Abdou Moumouni University, PO Box: 10 662, Niamey, Niger
Ibrahim Adamou Amoukou
Department of Biochemistry, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
Mousbahou Malam Alma
Department of Chemistry, Faculty of Sciences and Technology, Abdou Moumouni University, PO Box: 10 662, Niamey, Niger
Ramatou Seini Sidikou
Department of Biology, Faculty of Sciences and Technology, Abdou Moumouni University, PO Box: 10 662, Niamey, Niger
Mahamane Saadou
Department of Biology, Faculty of Sciences and Technology, Abdou Moumouni University, PO Box: 10 662, Niamey, Niger
Bilbis Lawal Suleman
Department of Biochemistry, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
ABSTRACT
Seeds from 17 varieties of Laganaria siceraria and 1 variety of Citrullus colocynthis were analyzed for their proximate amino acids composition. These seeds were produced on the Abdou Moumouni University, Faculty of Sciences and Technology, experimental field. The seeds showed significant difference in their protein content: Lagenaria siceraria (29.45 g/100 g), Citrullus colocynthis (12.40 g/100 g) but were closely related in their amino acids profile. Glutamic, aspartic acids and arginine were the most abundant amino acids while Total Sulphur Amino Acids (TSAA = methionine + cystine) were the limiting amino acids. Leucine was the most concentrated essential amino acid. The percentages of essential amino acids of the seeds were also closely related: Lagenaria siceraria (42.85%) and Citrullus colocynthis (41.51%). Despite a similar amino acids profile, the chemical concentrations of each amino acid were significantly different between varieties (p<0.001) exception of leucine (p = 0.06), showing that the varieties have some genetic differences. In L. siceraria seeds, TSAA ranged from 3.26% to 4.35% and Total Aromatic Amino Acids (TAAA) ranged from 27.14 to 30.55%. In C. colocynthis seeds the concentrations were 4.5 and 27.99%, respectively. When comparing the amino acids in these varieties with the recommended FAO/WHO provisional pattern, the seeds were adequate in histidine, TAAA, threonine and TSAA but supplementation was required for lysine in all varieties and for isoleucine, leucine and valine in some varieties. Predicted Protein Efficiency Ratios (PER) was 1.98 in C. colocynthis and ranged from 1.81 to 2.33 in L. siceraria.
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Haoua Sabo, Hassimi Sadou, Ibrahim Adamou Amoukou, Mousbahou Malam Alma, Ramatou Seini Sidikou, Mahamane Saadou and Bilbis Lawal Suleman, 2015. Determination and Comparison of the Amino Acid Composition of Seventeen Lagenaria siceraria Varieties and One Variety of Citrullus colocynthis Seed Flours. Pakistan Journal of Nutrition, 14: 100-106.
DOI: 10.3923/pjn.2015.100.106
URL: https://scialert.net/abstract/?doi=pjn.2015.100.106
DOI: 10.3923/pjn.2015.100.106
URL: https://scialert.net/abstract/?doi=pjn.2015.100.106
REFERENCES
- Adeyeye, E.I. and E.O. Afolabi, 2004. Amino acid composition of three different types of land snails consumed in Nigeria. Food Chem., 85: 535-539.
CrossRefDirect Link - Akobundu, E.N.T., J.P. Cherry and J.G. Simmons, 1982. Chemical, functional and nutritional properties of egusi (Colocynthis citrullus L.) seed protein products. J. Food Sci., 47: 829-835.
CrossRef - Alfawaz, M.A., 2004. Chemical composition and oil characteristics of pumpkin (Cucurbita maxima) seed kernels. Food Sci. Agric. Res. Bull., 129: 5-18.
Direct Link - Alsmeyer, R.H., A.E. Cunningham and M.L. Happich, 1974. Equations predict PER from amino acid analysis. Food Technol., 28: 34-38.
Direct Link - Aremu, M.O., O. Olaofe and T.E. Akintayo, 2006. A Comparative study on the chemical and amino acid composition of some Nigerian Under-utilized legume flours. Pak. J. Nutr., 5: 34-38.
CrossRefDirect Link - Aremu, M.O., O. Olaofe and C.A. Orjioke, 2008. Proximate and amino acid composition of bambara groundnut (Vigna subterranean), kersting's groundnut (Kerstingiella geocarpa) and scarlet runner bean (Phaseolus coccineus) protein concentrates. La Rivista Italiana Delle Sostanze Grasse, 85: 128-134.
Direct Link - El-Adawy, T.A. and K.M. Taha, 2001. Characteristics and composition of watermelon, pumpkin, and paprika seed oils and flours. J. Agric. Food Chem., 49: 1253-1259.
CrossRefDirect Link - Gurudeeban, S., K. Satyavani and T. Ramanathan, 2010. Bitter apple (Citrullus colocynthis): An overview of chemical composition and biomedical potentials. Asian J. Plant Sci., 9: 394-401.
CrossRefDirect Link - Rahman, A.S.H., 2003. Bottle gourd (Lagenaria siceraria) a vegetable for good health. Nat. Prod. Radiance, 2: 249-256.
Direct Link - Ige, M.M., A.O. Ogunsua and O.L. Okon, 1984. Functional properties of the proteins of some Nigerian oilseeds: Conophor seeds and three varieties of melon seeds. J. Agric. Food Chem., 32: 822-825.
CrossRefDirect Link - Mayakrishnan, V., S. Veluswamy, K.S. Sundaram, P. Kannappan and N. Abdullah, 2013. Free radical scavenging potential of Lagenaria siceraria (Molina) standl fruits extract. Asian Pacific J. Trop. Med., 6: 20-26.
CrossRefDirect Link - Ogundele, J.O., A.A. Oshodi and I.A. Amoo, 2012. Comparative study of amino acid and proximate composition of Citurllus colocynthis and Citrullus vulgaris seeds. Pak. J. Nutr., 11: 247-251.
CrossRefDirect Link - Ogundele, J.O., A.A. Oshodi, C.O. Fakunle, A.O. Oyebanji and I.A. Amoo, 2013. Evaluation and comparison of the amino acid composition of three varieties of Lagerena siceraria melon seed flours. Food Sci. Qual. Manage., 15: 36-42.
Direct Link - Ogungbenle, H.N., 2006. Chemical composition, functional properties and amino acid composition of some edible seeds. Rivista Italiana Delle Sostanze Grasse, 83: 81-86.
Direct Link - Olaofe, O., O.Y. Umar and G.O. Adediran, 1993. The effect of nematicides on the nutritive value and functional properties of cowpea seeds (Vigna unguiculata L. Walp). Food Chem., 46: 337-341.
CrossRefDirect Link - Olaofe, O., F.O. Adeyemi and G.O. Adediran, 1994. Amino acid and mineral compositions and functional properties of some oil seeds. J. Agric. Food Chem., 42: 878-881.
Direct Link - Oshodi, A.A., O. Olaofe and G.M. Hall, 1993. Amino acid, fatty acid and mineral composition of pigeon pea (Cajanus cajan). Int. J. Food Sci. Nutr., 43: 187-191.
CrossRefDirect Link - Ponka, R., E. Fokou, M. Fotso, M.B. Achu and M.F. Tchouanguep, 2005. Methods of preparation and the energy, protein and mineral values of three Cameroonian dishes: Corn chaff, Nnam Owondo/Ebobolo and Nnam Ngon/Ebobolo. Afr. J. Food Agric. Nutr. Dev., 5: 1-13.
Direct Link - Prajapati, R.P., M. Kalaria, S.K. Parmar and N.R. Sheth, 2010. Phytochemical and pharmacological review of Lagenaria sicereria. J. Ayu. Inte. Med., 1: 266-272.
Direct Link - Sadou, H., H. Sabo, M.M. Alma, M. Saadou and C.L. Leger, 2007. Chemical content of the seeds and physico-chemical characteristic of the seed oils from Citrullus colocynthis, Coccinia grandis, Cucumis metuliferus and Cucumis prophetarum of Niger. Bull. Chem. Soc. Ethiop., 21: 323-330.
Direct Link - Sathe, S.K., V. Iyer and D.K. Salunkhe, 1982. Functional properties of great northern bean (Phaseolus vulgaris L.) Proteins. Amino acid composition, in vitro digestibility and application to cookies. J. Food Sci., 47: 71-81.
CrossRef - Ullah, A.H., K. Sethumadhavan, C. Grimm and J. Shockey, 2012. Purification, characterization and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria. Adv. Biol. Chem., 2: 403-410.
Direct Link - Wuehler, S.E. and A.B. Hassoumi, 2011. Situational analysis of infant and young child nutrition policies and programmatic activities in Niger. Matern Child Nutr., 7: 133-156.
CrossRefDirect Link