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Pakistan Journal of Nutrition

Year: 2014 | Volume: 13 | Issue: 10 | Page No.: 600-609
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Research Article

Effect of Chemical Properties of Milk Thistle Seed Oil on Serum Lipid Profile and Antioxidants Capacity in Rats Fed High Cholesterol and Cholesterol Free Diets

I.R. Dabbour, M.N. Ahmad, H.R. Takruri and K.M. Al-Ismail

ABSTRACT

This study aimed to examine the in vivo effect of chemical properties of cold-pressed Milk Thistle Seed Oil (MTSO) on serum lipid profile, lipid peroxidation product (MDA) and total antioxidant capacity (TAC).These examinations were carried out to investigate the long-term effect of feeding two levels of MTSO in experimental diets with and without cholesterol. Sixty-four adult male Sprague-Dawley rats were divided into eight groups. Four groups were on free cholesterol diets with either 4% MTSO, 8% MTSO, 4% control (soybean oil) or 8% control oil. The other 4 groups were fed 1% cholesterol with percentages of oils similar to the first four groups for 12 weeks. Regarding serum lipoproteins there were significant differences (p<0.05) in TC in 8% MTSO and 8% control cholesterol-supplemented diets when compared with 8%control cholesterol-free diets. Also LDL-C of cholesterol-supplemented diet groups were significantly higher (p<0.05) than cholesterol-free diet groups. An interesting result was the significantly high levels (p<0.05) of HDL-C in rats fed cholesterol-free diet with MTSO 4 and 8% when compared to cholesterol-supplemented diets. There was a significantly higher (p<0.05) HDL-C/LDL-C ratio for cholesterol-free diets than cholesterol-supplemented diets. Subsequently, high significant difference (p<0.05) was observed in AI values at 8% cholesterol-supplemented diet when compared with cholesterol-free diet. MDA showed clearly high levels at 8% control oil with or without cholesterol. Regarding the TAC, it was higher (p<0.05) in 4% cholesterol-free MTSO diet when compared to 8% cholesterol-free control diet and 4% cholesterol supplemented MTSO diet. Based on the results obtained, the % of oil and presence of cholesterol were the main factors affecting the lipid profile, calculated AI, lipid peroxidation and TAC. With respect to in vivo lipid peroxidation, MTSO was clearly superior to soybean oil.
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How to cite this article

I.R. Dabbour, M.N. Ahmad, H.R. Takruri and K.M. Al-Ismail, 2014. Effect of Chemical Properties of Milk Thistle Seed Oil on Serum Lipid Profile and Antioxidants Capacity in Rats Fed High Cholesterol and Cholesterol Free Diets. Pakistan Journal of Nutrition, 13: 600-609.

DOI: 10.3923/pjn.2014.600.609

URL: https://scialert.net/abstract/?doi=pjn.2014.600.609

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REFERENCES

  1. Abu-Rajouh, K.S. and H.R. Takruri, 2000. Studing the nutritional value of milk thistle seeds. Arab J. Food Nutr., 2: 31-47.
  2. Aguilera, C.M., M.D. Mesa, M.C. Ramirez-Tortosa, M.T. Nestares, E. Ros and A. Gil, 2004. Sunflower oil does not protect against LDL oxidation as virgin olive oil does in patients with peripheral vascular disease. Clin. Nutr., 23: 673-681.
    CrossRefDirect Link
  3. Kris-Etherton, P.M., S. Innis and Ammerican Dietetic Assocition, Dietitians of Canada, 2007. Position of the American dietetic association and dietitians of Canada: Dietary fatty acids. J. Am. Diet. Assoc., 107: 1599-1611.
    PubMed
  4. Apak, R., K. Guclu, B. Demirata, M. Ozyurek and S.E. Celik et al., 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12: 1496-1547.
    CrossRefDirect Link
  5. Baba, N.H., Z. Ghossoub and Z. Habbal, 2000. Differential effects of dietary oils on plasma lipids, lipid peroxidation and adipose tissue lipoprotein lipase activity in rats. Nutr. Res., 20: 1113-1125.
    Direct Link
  6. Bail, S., G. Stuebiger, S. Krist, H. Unterweger and G. Buchbauer, 2008. Characterisation of various grape seed oils by volatile compounds, triacylglycerol composition, total phenols and antioxidant capacity. Food Chem., 108: 1122-1132.
    CrossRef
  7. Billett, M.A., J.S. Bruce, D.A. White, A.J. Bennett and A.M. Salter, 2000. Interactive effects of dietary cholesterol and different saturated fatty acids on lipoprotein metabolism in the hamster. Br. J. Nutr., 84: 439-447.
    CrossRefDirect Link
  8. Brown, D.W., A.H. Mokdad, H. Walke, M. As'ad and M. Al-Nsour et al., 2009. Projected burden of chronic, noncommunicable diseases in Jordan. Prev. Chronic Dis., Vol. 6.
    Direct Link
  9. Bartosz, G., 2003. Total antioxidant capacity. Adv. Clin. Chem., 37: 219-292.
  10. Bok, S.H., S.A. Lee, Y.B. Park, K.H. Bae, K.H. Son, T.S. Jeong and M.S. Choi, 1999. Plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutaryl-CoA reductase and Acyl CoA: Cholesterol transferase are lower in rats fed citrus peel extract or mixture of citrus bioflavonoids. J. Nutr., 129: 1182-1185.
  11. Cintra, D.E., A.G.V. Costa, M.D.C.G. Peluzio, S.L.P. Matta, M.T.C. Silva and N.M.B. Costa, 2006. Lipid profile of rats fed high-fat diets based on flaxseed, peanut, trout, or chicken skin. Nutrition, 22: 197-205.
    CrossRefDirect Link
  12. Dabbour, I.R., K.M. Al-Ismail, H.R. Takruri and F.S. Azzeh, 2014. Chemical characteristics and antioxidant content properties of cold pressed seed oil of wild milk thistle plant grown in Jordan. Pak. J. Nutr., 13: 67-78.
    CrossRefDirect Link
  13. Del Rio, D., A.J. Stewart and N. Pellegrini, 2005. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Metab. Cardiovasc. Dis., 15: 316-328.
    CrossRefDirect Link
  14. Fang, Y.C., B.H. Chen, R.F.S. Huang and Y.F. Lu, 2004. Effect of genistein supplementation on tissue genistein and lipid peroxidation of serum, liver and low-density lipoprotein in hamsters. J. Nutr. Biochem., 15: 142-148.
    CrossRefDirect Link
  15. Fathi-Achachlouei, B. and S. Azadmard-Damirchi, 2009. Milk thistle seed oil constituents from different varieties grown in Iran. J. Am. Oil Chem. Soc., 86: 643-649.
    CrossRefDirect Link
  16. Garjani, A., F. Fathiazad, A. Zakheri, N.A. Akbari and Y. Azarmie et al., 2009. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status and vascular function in hypercholesterolemic rats. J. Ethnopharmacol., 126: 525-532.
    CrossRefDirect Link
  17. Gilani, G.S., W.M. Ratnayake, S.P. Brooks, H.G. Botting, L.J. Plouffe and B.J. Lampi, 2002. Effects of dietary protein and fat on cholesterol and fat metabolism in rats. Nutr. Res., 22: 297-311.
    CrossRefDirect Link
  18. Gokkusu, C., T.A. Ozden and T. Mostafa, 2004. The relationship between oxidative stress and membrane ATPase enzyme activities in hypercholesterolemic rats supplemented with vitamin E. J. Med. Sci., 4: 70-76.
    CrossRefDirect Link
  19. Gorinstein, S., H. Leontowicz, M. Leontowicz, A. Lojek and M. Ciz et al., 2003. Seed oils improve lipid metabolism and increase antioxidant potential in rats fed diets containing cholesterol. Nutr. Res., 23: 317-330.
    CrossRefDirect Link
  20. Gurr, M.R., J.L. Harwood and K.N. Frayn, 2002. Lipid Biochemistry: An Introduction. 5th Edn., Wiley, New York, ISBN: 9780632054091, Pages: 340.
  21. Ha, T.Y., S. Han, S.R. Kim, I.H. Kim, H.Y. Lee and H.K. Kim, 2005. Bioactive components in rice bran oil improve lipid profiles in rats fed a high-cholesterol diet. Nutr. Res., 25: 597-606.
    CrossRefDirect Link
  22. Hammoudeh, A.J., M. Izraiq, E. Al-Mousa, H. Al-Tarawneh and A. El-Harassis et al., 2008. Serum lipid profiles with and without CAD: Jordan hyperlipidaemia and related targets Study [JoHARTS-1]. Eastern Mediterranean Health J., 14: 24-32.
    Direct Link
  23. Harvey, R.A., P.C. Champe and D.R. Ferrier, 2005. Biochemistry. 3rd Edn., Lippincott Williams and Wilkins, Philadelphia, USA.
  24. Hauenschild, A., R.G. Bretzel, H. Schnell-Kretschmer, H.U. Kloer, P.D. Hardt and N. Ewald, 2010. Successful treatment of severe hypertriglyceridemia with a formula diet rich in omega-3 fatty acids and medium-chain triglycerides. Ann. Nutr. Metab., 56: 170-175.
    CrossRefDirect Link
  25. Jentzsch, A.M., H. Bachmann, P. Furst and H.K. Biesalski, 1996. Improved analysis of malondialdehyde in human body fluids. Free Radical Biol. Med., 20: 251-256.
    CrossRefDirect Link
  26. Khader, Y.S., A. Batieha, M. Al-Khateeb, M. Al Omari and K. Ajlouni, 2010. Prevalence of dyslipidemia and its associated factors among Jordanian adults. J. Clin. Lipid, 4: 53-58.
    CrossRefDirect Link
  27. Kritchevsky, D. and S.C. Chen, 2005. Phytosterols-health benefits and potential concerns: A review. Nutr. Res., 25: 413-428.
    CrossRefDirect Link
  28. Lapenna, D., G. Ciofani, S.D. Pierdomenico, M.A. Giamberardino and F. Cuccurullo, 2001. Reaction conditions affecting the relationship between thiobarbituric acid reactivity and lipid peroxidesin human plasma. Free Radical Biol. Med., 31: 331-335.
    CrossRefDirect Link
  29. Makni, M., H. Fetoui, N.K. Gargouri, E.M. Garoui and H. Jaber et al., 2008. Hypolipidemic and hepatoprotective effects of flax and pumpkin seed mixture rich in ω-3 and ω-6 fatty acids in hypercholesterolemic rats. Food Chem. Toxicol., 46: 3714-3720.
    CrossRefDirect Link
  30. Martin-Carron, N., I. Goni, J.A. Larrauri, A. Garcia-Alonso and F. Saura-Calixto, 1999. Reduction in serum total and LDL cholesterol concentrations by a dietary fiber and polyphenol-rich grape product in hypercholesterolemic rats. Nutr. Res., 19: 1371-1381.
    CrossRefDirect Link
  31. Moon, K.D., S.S. Back, J.H. Kim, S.M. Jeon, M.K. Lee and M.S. Choi, 2001. Safflower seed extract lowers plasma and hepatic lipids in rats fed high-cholesterol diet. Nutr. Res., 21: 895-904.
    CrossRef
  32. Nevin, K.G. and T. Rajamohan, 2008. Influence of virgin coconut oil on blood coagulation factors, lipid levels and LDL oxidation in cholesterol fed Sprague-Dawley rats. e-SPEN, Eur. e-J. Clin. Nutr. Metab., 3: e1-e8.
    CrossRefDirect Link
  33. Niki, E., 2010. Assessment of antioxidant capacity in vitro and in vivo. Free Radical Biol. Med., 49: 503-515.
    CrossRefDirect Link
  34. Parry, J.W., Z. Cheng, J. Moore and L.L. Yu, 2008. Fatty acid composition, antioxidant properties and antiproliferative capacity of selected cold-pressed seed flours. J. Am. Oil Chem. Soc., 85: 457-464.
    CrossRefDirect Link
  35. Parry, J.W., Z. Hao, M. Luther, L. Su, K. Zhou and L.L. Yu, 2006. Characterization of cold-pressed onion, parsley, cardamom, mullein, roasted pumpkin and milk thistle seed oils. J. Am. Oil Chem., 83: 847-854.
    CrossRefDirect Link
  36. Reeves, P.G., 1997. Components of the AIN-93 diets as improvements in the AIN-76A diet. J. Nutr., 127: 838S-841S.
    PubMedDirect Link
  37. Salter, A.M., E.H. Mangiapane, A.J. Bennett, J.S. Bruce and M.A. Billett et al., 1998. The effect of different dietary fatty acids on lipoprotein metabolism: Concentration-dependent effects of diets enriched in oleic, myristic, palmitic and stearic acids. Br. J. Nutr., 79: 195-202.
    CrossRefDirect Link
  38. SAS., 2002. SAS User's Guide: Statistics. SAS Institute Inc., Cary, NC., USA.
  39. Skottova, N., R. Vecera, K. Urbanek, P. Vana, D. Walterova and L. Cvak, 2003. Effects of polyphenolic fraction of silymarin on lipoprotein profile in rats fed cholesterol-rich diets. Pharmacol. Res., 47: 17-26.
    CrossRefPubMedDirect Link
  40. Sobolova, L., N. Skottova, R. Vecera and K. Urbanek, 2006. Effect of silymarin and its polyphenolic fraction on cholesterol absorption in rats. Pharmacol. Res., 53: 104-112.
    CrossRefPubMedDirect Link
  41. Tuberoso, C.I., A. Kowalczyk, E. Sarritzu and P. Cabras, 2007. Determination of antioxidant compounds and antioxidant activity in commercial oilseeds for food use. Food Chem., 103: 1494-1501.
    CrossRefDirect Link
  42. Visavadiya, N.P. and A.V.R.L. Narasimhacharya, 2008. Sesame as a hypocholesteraemic and antioxidant dietary component. Food Chem. Toxicol., 46: 1889-1895.
    CrossRefPubMedDirect Link
  43. Whent, M., M. Slavin, W. Kenworthy and L. Yu, 2010. Potential relationships between fatty acid compositions and phytochemicals of selected low linolenic soybeans grown in Maryland. J. Am. Oil Chem. Soc., 87: 549-558.
    CrossRefDirect Link

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