Submit Manuscript

Pakistan Journal of Nutrition

Year: 2014 | Volume: 13 | Issue: 10 | Page No.: 584-588
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Effect of Blood Leaf (Iresine herbstii) Leaf Extract and Powder on the Biochemical Profile of Adult Male Albino Wistar Rats

E.U. Madukwe, A.M. Okafor and C.M. Enemkpali

ABSTRACT

This study assessed effect of Iresine herbstii leaf extract and powder on biochemical profile of adult male albino Wistar rats. Twenty adult male albino Wistar rats divided into four groups of five rats each were used for the study. All the animals received rat-chow and water ad-libitum. Group A was the control. Groups B, C and D received in addition, fresh leaf extract, shade-dried leaf extract and shade-dried leaf powder, respectively. Blood samples were obtained from the rats for lipid profile, liver and kidney function tests. Data obtained were analyzed using Statistical Package for Social Sciences (SPSS) for windows version 18. p<0.05 was accepted as level of significance. Serum AST was significantly (p<0.05) increased in rats fed fresh leaf extract. There was a significant (p<0.05) reduction in serum ALT of rats fed shade-dried leaf extract and powder. However, serum ALT of rats fed fresh leaf extract was significantly (p<0.05) increased. Serum ACP was significantly (p<0.05) increased in the three treatment groups. Serum creatinine was significantly (p<0.05) increased in rats fed fresh leaf extract. Rats fed shade-dried leaf powder showed a significant (p<0.05) increase in serum urea after treatment. Serum urea of rats fed fresh leaf extract was slightly reduced. There was a significant (p<0.05) increase in total cholesterol of rats fed fresh leaf extract whereas rats fed shade-dried leaf powder showed a significant (p<0.05) decrease in total cholesterol. Serum LDL-C of three treatment groups were increased. However, this was only significant (p<0.05) in rats fed shade-dried leaf extract.
PDF References Citation

Search

REFERENCES

  1. Ademuyiwa, O., R.N. Ugbaja, F. Idumebor and O. Adebawo, 2005. Plasma lipid profiles and risk of cardiovascular disease in occupational lead exposure in Abeokuta, Nigeria. Lipids Health Dis., Vol. 4.
    CrossRefDirect Link
  2. Amadi, B., E.N. Agomuo and M.K.C. Duru, 2013. Toxicological studies of Asmina triloba leaves on haematology, liver, kidney using rat model. Int. Sci. Res. J., 4: 11-17.
  3. Amagloh, F.K. and A. Benang, 2009. Effectiveness of Moringa oleifera seed as coagulant for water purification. Afr. J. Agric. Res., 4: 119-123.
    Direct Link
  4. Anderson, A.J., 1968. The effect of anti-inflammatory drugs on the enzymic activity of a rat liver granular fraction which increases vascular permeability. Biochem. Pharmacol., 17: 2253-2264.
    CrossRefDirect Link
  5. Balint, T., J. Ferenczy, F. Katai, I. Kiss and L. Kraczer et al., 1997. Similarities and differences between the massive eel (Anguilla anguilla L.) Devastations that occurred in lake balaton in 1991 and 1995. Ecotoxicol. Environ. Safety, 37: 17-23.
    CrossRefDirect Link
  6. Bergmeyer, H.U., P. Scheibe and A.W. Wahlefeld, 1978. Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clin. Chem., 24: 58-73.
    PubMedDirect Link
  7. Brenner, B.M. and C. Floyd, 1999. The Kidney. 6th Edn., W.B. Saunders Co., Philadelphia, PA.
  8. Burtis, C.A. and R.A. Edward, 1999. Tietz Textbook of Clinical Chemistry. 3rd Edn., W.B. Saunders, Philadelphia, PA., ISBN: 9780721656106, Pages: 1917.
    Direct Link
  9. Dragsted, L.O., B. Krath, G. Ravn-Haren, U.B. Vogel and A.M. Vinggaard et al., 2006. Symposium on phytochemicals: Biological effects of fruits and vegetables. Proc. Nutr. Soc., 65: 61-67.
  10. Henry, J.B., 2001. Clinical Diagnosis and Management by Laboratory Methods. 20th Edn., W.B. Saunders Co., Philadelphia, PA.
  11. Ilango, K. and V. Chitra, 2009. Hepatoprotective and antioxidant activities of fruit pulp of Limonia acidissima Linn. Int. J. Health Res., 2: 361-367.
  12. Jenkins, D.J., T.M. Wolever, A.R. Leeds, M.A. Gassull and P. Haisman et al., 1978. Dietary fibres, fibre analogues and glucose tolerance: Importance of viscosity. Br. Med. J., 1: 1392-1394.
    CrossRefPubMedDirect Link
  13. Rajagopal, S.K., P. Manickam, V. Periyasamy and N. Namasivayam, 2003. Activity of Cassia auriculata leaf extract in rats with alcoholic liver injury. J. Nutr. Biochem., 14: 452-458.
    CrossRefDirect Link
  14. Law, M.R., 1999. Lowering heart disease risk with cholesterol reduction: Evidence from observational studies and clinical trials. Eur. Heart J. Suppl., 1: S3-S8.
    Direct Link
  15. Lichtenstein, A.H., L.J. Appel, M. Brands, M. Carnethon and S. Daniels et al., 2006. Summary of American heart association diet and lifestyle recommendations revision 2006. Arterioscler. Thromb. Vasc. Biol., 26: 2186-2191.
    CrossRef
  16. Orech, F.O., T. Akenga, J. Ochora, H. Friis and J. Aagaard-Hansen, 2005. Potential toxicity of some traditional leafy vegetables consumed in Nyangoma Division, Western Kenya. Afr. J. Food Nutr. Sci., 5: 1-13.
    Direct Link
  17. Pagana, K.D., 1998. Mosby's Manual of Diagnostic and Laboratory Tests. Mosby Inc., St. Louis., USA.
  18. Ramachandra, S.S., A.A. Quereshi, S.A.H. Viswanath, T. Patil and T. Prakesh et al., 2007. Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia, 78: 451-454.
    PubMed
  19. Ranjna, C., 1999. Practical Clinical Biochemistry Methods and Interpretation. 2nd Edn., Jaypee Brothers Medical Publishers (p) Ltd., India, pp: 117.
  20. Rodwell, V.W. and P.J. Kennelly, 2000. Enzymes: General Properties. In: Harper's Biochemistry, Murray, R.K., D.K. Granner, P.A. Mayes and V.W. Rodwell, (Eds.). 25 Edn., McGrawHill, New York, pp: 74-85.
  21. Saka, W.A., R.E. Akhigbe, O.T. Popoola and O.S. Oyekunle, 2012. Changes in serum electrolytes, urea and Creatinine in Aloe Vera-treated rats. J. Young Pharm., 4: 78-81.
    CrossRef
  22. Das, S.K., S. Varadhan, L. Dhanya, S. Mukherjee and D.M. Vasudevan, 2008. Effects of chronic ethanol exposure on renal function tests and oxidative stress in kidney. Indian J. Clin. Biochem., 23: 341-344.
    CrossRef
  23. Wallach, J.B., 2007. Interpretation of Diagnostic Tests. 7th Edn., Lippincott Williams and Wilkins, Philadelphia, PA., ISBN: 9780781730556, Pages: 1181.
  24. Wolf, P.L., 1999. Biochemical diagnosis of liver disease. Indian J. Clin. Biochem., 14: 59-90.
    Direct Link

Search

Leave a Comment

Your email address will not be published. Required fields are marked *