E.U. Madukwe
Department of Home Science, Nutrition and Dietetics, University of Nigeria, Nsukka, Nigeria
A.M. Okafor
Department of Home Science, Nutrition and Dietetics, University of Nigeria, Nsukka, Nigeria
V.U. Ogbonna
Department of Home Science, Nutrition and Dietetics, University of Nigeria, Nsukka, Nigeria
ABSTRACT
This study evaluated effectiveness of fresh and shade-dried Mucuna pruriens leaf extract in managing anaemia in adult male albino rats. Fresh leaves of Mucuna pruriens were harvested from Enugu-Ezike, Enugu state, Nigeria. The leaves were used to prepare fresh and shade-dried leaf extracts which were subjected to chemical analysis using standard analytical methods. Fifteen adult male albino rats weighing 180-250g, grouped into three groups (A, B and C) of five rats each were used for the study. All groups received rat chow and water ad-libitum. Group B and C received in addition, the fresh and shade-dried Mucuna pruriens leaf extracts respectively after anaemia induction. Blood samples were collected from the rats for determination of haemoglobin, PCV, RBC and WBC after a 5-day acclimatization, after anaemia induction and at the end of the study. Statistical Package for Social Sciences (SPSS) for windows version 18 was used to analyze the data obtained. p<0.05 was accepted as a cut-off for significant level. Fresh and shade-dried Mucuna pruriens leaf extracts contained iron (9.00±0.28 and 3.50±0.00 mg/100 mL), vitamin C (18.45±2.19 and 31.35±0.35 mg/100 mL) and pro-vitamin A (100.21±0.28 and 170.21±0.28 mg/100 mL). Haemoglobin, packed cell volume and white blood cell of rats fed fresh Mucuna pruriens leaf extract significantly (p<0.05) increased after treatment. Shade-dried Mucuna pruriens leaf extract significantly (p<0.05) increased red blood cell and white blood cell of the rats after treatment. Lymphocytes of the anaemic rats fed fresh and shade-dried Mucuna pruriens leaf extracts was significantly (p<0.05) increased whereas there was no significant (p>0.05) increase in the eosinophils of the anaemic rats.
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How to cite this article
E.U. Madukwe, A.M. Okafor and V.U. Ogbonna, 2014. Effectiveness of Fresh and Shade-Dried Mucuna pruriens Leaf Extract in Controlling Anaemia in Adult Male Albino Rats. Pakistan Journal of Nutrition, 13: 579-583.
DOI: 10.3923/pjn.2014.579.583
URL: https://scialert.net/abstract/?doi=pjn.2014.579.583
DOI: 10.3923/pjn.2014.579.583
URL: https://scialert.net/abstract/?doi=pjn.2014.579.583
REFERENCES
- Agbor, A.G., E.O. Oben and J.Y. Ngogang, 2005. Haematinic activity of Hibiscus cannabinus. Afr. J. Biotechnol., 4: 833-837.
Direct Link - Arollado, E.C. and M.O. Osi, 2010. Hematinic activity of Alternanthera sessilis (L.) R. Br. (Amaranthaceae) in mice and rats. E-Int. Sci. Res. J., 2: 110-117.
Direct Link - George-Gay, B. and K. Parker, 2003. Understanding the complete blood count with differential. J. Peri-Anesthesia Nurs., 18: 96-117.
Direct Link - Muchoki, C.M., P.O. Lamuka and J.K. Imungi, 2010. Reduction of nitrates, oxalates and phenols in fermented solar-dried stored cowpea (Vigna unguiculata L.) leaf vegetables. Afr. J. Food Agric. Nutr. Dev., 10: 4398-4412.
Direct Link - Okochi, V.I., J. Okpuzor and L.A. Alli, 2003. Comparison of an African herbal formula with commercially available haematinics. Afr. J. Biotechnol., 2: 237-240.
Direct Link - Olaniyan, M.F. and A. Adeleke, 2005. A study of the effect of pumpkin (UGU-Telfaira occidentals) milk and raw egg mixture in the treatment of anaemic pregnant women in a rural area. Afr. J. Tradit. Complement. Altern. Med., 2: 269-273.
Direct Link - Saba, A.B., A.A. Oyagbemi and O.I. Azeez, 2010. Antidiabetic and haematinic effects of Parquetina nigrescens on alloxan induced type-1 diabetes and normocytic normochromic anaemia in Wistar rats. Afr. Health Sci., 10: 276-282.
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