Diabetes mellitus is a metabolic disorder that is characterized by hyperglycemia and associated with increased oxidative stress. The polyphenol property in pomegranate juice was reported to have significant antioxidant effect. The present study aims to investigate the hypoglycemic and antioxidative effect of pomegranate juice in streptozotocin-induced diabetic rats. Twenty healthy male rattus norvegicus with age of eight weeks and weighing at average of 200 g were randomly assigned into four groups. Diabetic models were induced by using 60 mg/kg body weight (BW) streptozotocin (STZ) intraperitoneally. The rats were allocated into four groups, group treated with 0.1 mg/kg BW glibenclamide, groups supplemented with 1, 2 and 4 mL/200 g BW pomegranate juice, respectively. Evaluation of hypoglycemic and antioxidative effect were performed by measuring blood glucose level, lipid peroxidation, including malondialdehyde (MDA) and advanced glycation end-product (AGE) before and after treatment. Lipid profile was also measured before and after treatment. Treatment with glibenclamide and supplementation with three different doses of pomegranate juice in diabetic rats were significantly reduced blood glucose level. Supplementation with 2 mL/200 g BW pomegranate juice was significantly reduced MDA concentration, whereas the AGE concentration was not significantly decreased by pomegranate juice supplementation. Lipid profile related to total cholesterol and triglyceride levels were not significantly reduced. Furthermore, high density lipoprotein was not increased significantly after each treatment. Pomegranate juice has hypoglycemic and antioxidative effects in streptozotocin-induced diabetic rats.
PDF References Citation
How to cite this article
Prasetyastuti, Mochammad Willy Pratama Anthony, Nuha Aulia Rahman, Ngadikun and Sunarti, 2014. Hypoglycemic and Antioxidative Effects of Pomegranate (Punica granatum L.) Juice in Streptozotocin Induced Diabetic Rats. Pakistan Journal of Nutrition, 13: 567-572.
- Atilgan, D., B. Parlaktas, N. Uluocak, Y. Gencten and F. Erdemir et al., 2014. Pomegranate (Punica granatum) juice reduces oxidative injury and improves sperm concentration in a rat model of testicular torsion-detorsion. Exp. Ther. Med., 8: 478-482.
- Das, S. and S. Barman, 2012. Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non-insulin-dependent diabetes mellitus albino rats. Indian J. Pharmacol., 44: 219-224.
- De, D., K. Chatterjee, K.M. Ali, T.K. Bera and D. Ghosh, 2011. Antidiabetic potentiality of the aqueous-methanolic extract of seed of Swietenia mahagoni (L.) Jacq. in streptozotocin-induced diabetic male albino rat: A correlative and evidence-based approach with antioxidative and antihyperlipidemic activities. Evid. Based Complem. Altern. Med.
- De Nigris, F., M.L. Balestrieri, S. Williams-Ignarro, F.P. D'Armiento, C. Fiorito, L.J. Ignarro and C. Napoli, 2007. The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats. Nitric Oxide, 17: 50-54.
- Jarald, E., S.B. Joshi and D.C. Jain, 2008. Diabetes and herbal medicines. Iran. J. Pharmacol. Ther., 7: 97-106.
- Khalil, E.A.M., 2004. Antidiabetic effect of an aqueous extract of pomegranate (Punica granatum L.) peels in normal and alloxan diabetic rats. Egypt. J. Hosp. Med., 16: 92-99.
- Li, Y.M., T. Mitsuhashi, D. Wojciechowicz, N. Shimizu and J. Li et al., 1996. Molecular identity and cellular distribution of advanced glycation endproduct receptors: Relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci., 93: 11047-11052.
- Omar, H.S., H.A. El-Beshbishy, Z. Moussa, K.F. Taha and A.N.B. Singab, 2011. Antioxidant activity of Artocarpus heterophyllus Lam.(Jack Fruit) leaf extracts: Remarkable attenuations of hyperglycemia and hyperlipidemia in streptozotocin-diabetic rats. Scient. World J., 11: 788-800.
- Shinohara, M., P.J. Thornalley, I. Giardino, P. Beisswenger, S.R. Thorpe, J. Onorato and M. Brownlee, 1998. Overexpression of glyoxalase-I in bovine endothelial cells inhibits intracellular advanced glycation endproduct formation and prevents hyperglycemia-induced increases in macromolecular endocytosis. J. Clin. Invest., 101: 1142-1147.
- Tjakradidjaja, F.A. and A.S. Tjakradidjaja, 2011. Pomegranate (Punica granatum L) powder reduced malondialdehyde (MDA) level in cigarette smoke exposed rats. Med. J. Indonesia, 20: 34-39.
- Ueno, Y., M. Kizaki, R. Nakagiri, T. Kamiya, H. Sumi and T. Osawa, 2002. Dietary glutathione protects rats from diabetic nephropathy and neuropathy. J. Nutr., 132: 897-900.