Subscribe Now Subscribe Today
Research Article
 

Antioxidant, Hepatoprotective and Antiatherogenic Effects of Curcumin on High Fat Diet Induced Dyslipidemia in Rats



Nazish Iqbal Khan and Tabassum Mahboob
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

Present study is designed to investigate the atheroprotective potential of curcumin in rat models of diet induced dyslipidemia. Twenty four wistar rats of same age and sex were selected for the study and divided into four experimental groups with six animals per group. Group I was control untreated rats, group II rats received high fat diet for eight weeks, group III received 0.2 g/kg of body weight/day curcumin together with fat diet and group IV had only curcumin administration for upto four weeks. Results showed that curcumin treatment with or without fat diet administration significantly reduce plasma lipid levels, ALT, AST, ALP and glucose concentrations. High fat diet administration significantly suppresses levels of catalase, SOD and glutathione in these tissues. Whereas MDA levels were significantly raised. Curcumin treatment improve these tissue antioxidant enzyme status together with decreased MDA levels. These results suggests that daily dietary intake of curcumin in recommended doses may be helpful in restoring lipid disorders and maintaining body’s antioxidant status in patients of cardiovascular diseases.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Nazish Iqbal Khan and Tabassum Mahboob, 2014. Antioxidant, Hepatoprotective and Antiatherogenic Effects of Curcumin on High Fat Diet Induced Dyslipidemia in Rats. Pakistan Journal of Nutrition, 13: 537-545.

DOI: 10.3923/pjn.2014.537.545

URL: https://scialert.net/abstract/?doi=pjn.2014.537.545

REFERENCES
1:  Aoki, H., Y. Takada, S. Kondo, R. Sawaya, B.B. Aggarwal and Y. Kondo, 2007. Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: Role of Akt and extracellular signal-regulated kinase signaling pathways. Mol. Pharmacol., 72: 29-39.
CrossRef  |  Direct Link  |  

2:  Babu, P.S. and K. Srinivasan, 1997. Hypolipidemic action of curcumin, the active principle of turmeric (Curcuma longa) in streptozotocin induced diabetic rats. Mol. Cell. Biochem., 166: 169-175.
CrossRef  |  PubMed  |  Direct Link  |  

3:  Bairaktari, E.T., K.I. Seferiadis and M.S. Elisaf, 2005. Evaluation of methods for the measurement of low-density lipoprotein cholesterol. J. Cardiovasc. Pharmacol. Therapeut., 10: 45-54.
CrossRef  |  

4:  Baum, I. and A. Ng, 2004. Curcumin interaction with copper and iron suggests one possible mechanism of action in Alzheimer's disease animal models. J. Alzheimers Dis., 6: 367-377.
CrossRef  |  Direct Link  |  

5:  Bergmeyer, H.U., 1980. IFCC methods for the measurement of catalytic concentrations of enzymes: Part 3. IFCC method for alanine aminotransferase (L-alanine: 2-oxoglutarate aminotransferase, EC 2.6.1.2). Clinica Chimica Acta, 105: 147-154.
CrossRef  |  

6:  Calderon, R.M., L.X. Cubeddu, R.B. Goldberg and E.R. Schiff, 2010. Statins in the treatment of dyslipidemia in the presence of elevated liver aminotransferase levels: A therapeutic dilemma. Mayo Clin. Proc., 85: 349-356.
CrossRef  |  

7:  Carlberg, I. and B. Mannervik, 1985. Glutathione reductase. Methods Enzymol., 113: 484-490.
PubMed  |  

8:  Chattopadhyay, I., K. Biswas, U. Bandyopadhyay and R.K. Banerjee, 2004. Turmeric and curcumin: Biological actions and medicinal applications. Curr. Sci., 87: 44-53.
Direct Link  |  

9:  Chaturvedi, T.P., 2009. Uses of turmeric in dentistry: An update. Indian J. Dental Res., 20: 107-109.
PubMed  |  Direct Link  |  

10:  Deby-Dupont, G., A. Mouithys-Mickalad, D. Serteyn, M. Lamy and C. Deby, 2005. Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells. Biochem. Biophys. Res. Commun., 333: 21-27.
CrossRef  |  

11:  Delahoy, P.J., D.J. Magliano, K. Webb, M. Grobler and D. Liew, 2009. The relationship between reduction in low-density lipoprotein cholesterol by statins and reduction in risk of cardiovascular outcomes: An updated meta-analysis. Clin. Therapeut., 31: 236-244.
CrossRef  |  

12:  Durgaprasad, S., C.G. Pai, J.F. Alvres and S. Namitha, 2005. A pilot study of the antioxidant effect of curcumin in tropical pancreatitis. Indian J. Med. Res., 122: 315-318.
PubMed  |  

13:  Flegg, H.M., 1973. An investigation of the determination of serum cholesterol by an enzymatic method. Ann. Clin. Biochem.: Int. J. Lab. Med., 10: 79-84.
CrossRef  |  Direct Link  |  

14:  Fossati, P. and L. Prencipe, 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28: 2077-2080.
CrossRef  |  PubMed  |  Direct Link  |  

15:  Friedewald, W.T., R.I. Levy and D.S. Fredrickson, 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem., 18: 499-502.
CrossRef  |  PubMed  |  Direct Link  |  

16:  Garcea, G., D.P. Berry, D.J.L. Jones, R. Singh and A.R. Dennison et al., 2005. Consumption of the putative chemopreventive agent curcumin by cancer patients: Assessment of curcumin levels in the colorectum and their pharmacodynamic consequences. Cancer Epidemiol. Biomarkers Prev., 14: 120-125.
Direct Link  |  

17:  Garcea, G., D.J. Jones, R. Singh, A.R. Dennison and P.B. Farmer et al., 2004. Detection of curcumin and its metabolites in hepatie lissue and portal blood of partients following oral administration. Br. J. Cancer, 90: 1011-1015.
PubMed  |  

18:  Gupta, S.C., S. Patchva and B.B. Aggarwal, 2013. Therapeutic roles of curcumin: Lessons learned from clinical trials. Am. Associ. Pharm. Sci. J., 15: 195-218.
CrossRef  |  Direct Link  |  

19:  Ide, H., S. Tokiwa, K. Sakamaki, K. Nishio and S. Isotani et al., 2010. Combined inhibitory effects of soy isoflavones and curcumin on the production of prostate-specific antigen. Prostate, 70: 1127-1133.
CrossRef  |  PubMed  |  

20:  Jang, E.M., M.S. Choi, U.J. Jung, M.J. Kim and H.J. Kim et al., 2008. Beneficial effects of curcumin on hyperlipidemia and insulin resistance in high-fat-fed hamster. Metabolism, 57: 1576-1583.
PubMed  |  

21:  Kamat, A.M., G. Sethi and B.B. Aggarwal, 2007. Curcumin potentiates the apoptotic effects of chemotherapeutic agents and cytokines through down-regulation of nuclear factor-κB and nuclear factor-κB-regulated gene products in IFN-α-sensitive and IFN-α-resistant human bladder cancer cells. Mol. Cancer Therapeut., 6: 1022-1030.
CrossRef  |  PubMed  |  

22:  Kapoor, P., M.N. Ansari and U. Bhandari, 2008. Modulatory effect of curcumin on methionine-induced hyperlipidemia and hyperhomocysteinemia in albino rats. Indian J. Exp. Biol., 46: 534-540.
PubMed  |  

23:  Khalifa, M.M.A., S.A. Mangoura, M.A. El-Moselhy and M.E. El-Daly, 2009. The effectiveness of β adrenoceptor antagonists on progression of insulin resistance and type 2 diabetes in high fat diet-fed rats. Saudi Pharmaceut. J., 17: 19-28.

24:  Kita, T., N. Kume, M. Minami, K. Hayashida and T. Murayama et al., 2001. Role of oxidized LDL in atherosclerosis. Ann. N. Y. Acad. Sci., 947: 199-206.
CrossRef  |  

25:  Kono, Y., 1978. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch. Biochem. Biophys., 186: 189-195.
CrossRef  |  PubMed  |  Direct Link  |  

26:  Lin, Y.G., A.B. Kunnumakkara, A. Nair, W.M. Merritt and L.Y. Han et al., 2007. Curcumin inhibits tumor growth and angiogenesis in ovarian carcinoma by targeting the nuclear factor-κB pathway. Clin. Cancer Res., 13: 3423-3430.
PubMed  |  

27:  Manikandan, P., M. Sumitra, S. Aishwarya, B.M. Manohar, B. Lokanadam and R. Puvanakrishnan, 2004. Curcumin modulates free radical quenching in myocardial ischaemia in rats. Int. J. Biochem. Cell Biol., 36: 1967-1980.
CrossRef  |  PubMed  |  

28:  Marinangeli, C.P. and P.J. Jones, 2010. Plant sterols, marine-derived omega-3 fatty acids and other functional ingredients: A new frontier for treating hyperlipidemia. Nutr. Metab., Vol. 7. 10.1186/1743-7075-7-76

29:  Meghana, K., G. Sanjeev and B. Ramesh, 2007. Curcumin prevents streptozotocin-induced islet damage by scavenging free radicals: A prophylactic and protective role. Eur. J. Pharm., 577: 183-191.
CrossRef  |  Direct Link  |  

30:  Mohanty, I., D.S. Arya, A. Dinda, S. Joshi, K.K. Talwar and S.K. Gupta, 2004. Protective effects of Curcuma longa on ischemia-reperfusion induced myocardial injuries and their mechanisms. Life Sci., 75: 1701-1711.
PubMed  |  

31:  Murugan, P. and L. Pari, 2005. Effect of tetrahydrocurcumin on erythromycin estolate-induced lipid peroxidation in rats. J. Basic Clin. Physiol. Pharmacol., 16: 1-16.
CrossRef  |  

32:  Murugan, P. and L. Pari, 2006. Effect of tetrahydrocurcumin on lipid peroxidation and lipids in streptozotocin-nicotinamide-induced diabetic rats. Basic Clin. Pharmacol. Toxicol., 99: 122-127.
PubMed  |  

33:  Nishiyama, T., T. Mae, H. Kishida, M. Tsukagawa and Y. Mimaki et al., 2005. Curcuminoids and sesquiterpenoids in turmeric (Curcuma longa L.) suppress and increase in blood glucose level in type 2 diabetic KK-Ay mice. J. Agric. Food Chem., 53: 959-963.
CrossRef  |  PubMed  |  

34:  Noori, S., K. Nasir and T. Mahboob, 2009. Effects of cocoa powder on oxidant/ antioxidant status in liver, heart and kidney tissues of rats. J. Anim. Plant Sci., 19: 174-178.
Direct Link  |  

35:  Ohkawa, H., N. Ohishi and K. Yagi, 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351-358.
CrossRef  |  PubMed  |  Direct Link  |  

36:  Quiles, J.L., C. Aguilera, M.D. Mesa, M.C. Ramirez-Tortosa, L. Baro and A. Gil, 1998. An ethanolic-aqueous extract of Curcuma longa decreases the susceptibility of liver microsomes and mitochondria to lipid peroxidation in atherosclerotic rabbits. Biofactors, 8: 79-85.
Direct Link  |  

37:  Quiles, J.C., M.D. Mesa, C.L. Ramirez-Tortosa, C.M. Auiglera, M. Battino, A. Gil and M.C. Ramirez-Tortosa, 2002. Curcuma longa extract supplementation reduces oxidative stress and attenuates aortic fatty streak development in rabbits. Arterioscler. Thromb. Vasc. Biol., 22: 1225-1231.
CrossRef  |  Direct Link  |  

38:  Ramirez-Tortosa, M.C., M.D. Mesa, M.C. Aguilera, J.L. Quiles and L. Baro et al., 1999. Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atherosclerosis, 147: 371-378.
CrossRef  |  PubMed  |  Direct Link  |  

39:  Seigler, L. and W.T. Wu, 1981. Separation of serum high-density lipoprotein for cholesterol determination: Ultracentrifugation vs precipitation with sodium phosphotungstate and magnesium chloride. Clin. Chem., 27: 838-841.
PubMed  |  Direct Link  |  

40:  Sinha, A.K., 1972. Colorimetric assay of catalase. Anal. Biochem., 47: 389-394.
CrossRef  |  PubMed  |  Direct Link  |  

41:  Skommer, J., D. Wlodkowic and J. Pelkonen, 2007. Gene-expression profiling during curcumin-induced apoptosis reveals downregulation of CXCR4. Exp. Hematol., 35: 84-95.
CrossRef  |  

42:  Tietz, N.W., A.D. Rinker and L.M. Shaw, 1983. IFCC methods for the measurement of catalytic concentration of enzymes Part 5. IFCC method for alkaline phosphatase (orthophosphoric-monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1). J. Clin. Chem. Clin. Biochem., 21: 731-748.
PubMed  |  Direct Link  |  

43:  Tomkin, G.H. and D. Owens, 2012. LDL as a cause of atherosclerosis. Open Atheroscler. Thromb. J., 5: 13-21.
Direct Link  |  

44:  Umeshchandra, S., D.G. Umeshchandra and S.M. Awanti, 2012. Atherogenic Index of plasma (AIP) in post menopausal women. Res. J. Pharm. Biol. Chem. Sci., 3: 519-523.

45:  Weisberg, S.P., R. Leibel and D.V. Tortoriello, 2008. Dietary curcumin significantly improves obesity-associated inflammation and diabetes in mouse models of diabesity. Endocrinology, 149: 3549-3558.
CrossRef  |  PubMed  |  Direct Link  |  

46:  Yang, X., D.P. Thomas, X. Zhang, B.W. Culver and B.M. Alexander et al., 2006. Curcumin inhibits platelet-derived growth factor-stimulated vascular smooth muscle cell function and injury-induced neointima formation. Arterioscler. Thromb. Vasc. Biol., 26: 85-90.
CrossRef  |  Direct Link  |  

47:  Yeh, C.H., T.P. Chen, Y.C. Wu, Y.M. Lin and P.J. Lin, 2005. Inhibition of NFκB activation with curcumin attenuates plasma inflammatory cytokines surge and cardiomyocytic apoptosis following cardiac ischemia/reperfusion. J. Surg. Res., 125: 109-116.
CrossRef  |  PubMed  |  Direct Link  |  

©  2020 Science Alert. All Rights Reserved