Subscribe Now Subscribe Today
Research Article
 

Free Radical Scavenging and Antibacterial Activity of Crude Extracts from Selected Plants of Medicinal Value Used in Zululand



E. Muleya, A.S. Ahmed, A.M. Sipamla and F.M. Mtunzi
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

Ledebouria revoluta, Berkheya setifera and Carissa bispinosa are some of the medicinal plants used in South African traditional medicine. An investigation to evaluate antimicrobial, anti-inflammatory and free radical scavenging activities of the root crude extracts and fractions from the three plants was carried out. Reduction by 2, 2-azinobis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS.+) and 2, 2-di (4-tert-octylphenyl)-1-picrylhydrazyl (DPPH.) radicals were used to assess antioxidant capacities by change in absorbance in a UV-VIS spectrophotometer. Anti-inflammatory activity of the plant extracts against 15-soybean derived lipoxygenase (15-LOX) enzyme was evaluated as increase in absorbance at 234 nm after addition of soya bean derived 15-LOX, using linoleic acid (134 µM) as substrate. Anti-microbial activities were determined by assessing the plant samples’Minimum Inhibitory Concentrations (MICs) by a micro dilution method. Carissa bispinosa and Berkheya setifera have shown good to moderate antimicrobial activity. Ledebouria revoluta displayed good activities (20 µg/mL) for both acetone and methanol fractions against E. coli and good-moderate activity (160-320 µg/mL) against the rest of the bacterial strains used. Anti-fungal activity for Ledebouria revoluta methanol fraction (20 µg/mL) is high against Candida albicans. Percentage inhibition of 15-soybean lipoxygenase enzyme by the crude extracts at concentration of 25 µg/mL was for Berkheya setifera, 80%, Carissa bispinosa, 65% and Ledebouria revoluta, 40%.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

E. Muleya, A.S. Ahmed, A.M. Sipamla and F.M. Mtunzi, 2014. Free Radical Scavenging and Antibacterial Activity of Crude Extracts from Selected Plants of Medicinal Value Used in Zululand. Pakistan Journal of Nutrition, 13: 38-42.

DOI: 10.3923/pjn.2014.38.42

URL: https://scialert.net/abstract/?doi=pjn.2014.38.42

REFERENCES
1:  Abegaz, B.M., 2002. Novel phenylanthraquinoine, isofuranonaphthoquinones, homoisoflavonoids and biflavonoids from African plants in the genera. Bulbine Scilla Ledrebouria Rhus Phytochem. Rev., 1: 299-310.

2:  Dold, A.P. and M.L. Cocks, 2001. A succulent herbal: The medicinal and cultural use of some succulent plants traded in the Eastern Cape Province of South Africa. Cactus and Succulent J., 73: 141-145.

3:  Dold, A.P. and M.L. Cocks, 2002. The trade in medicinal plants in the Eastern Cape Province, South Africa. S. Afr. J. Sci., 98: 589-597.
Direct Link  |  

4:  Eloff, J.N., 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica, 64: 711-713.
CrossRef  |  PubMed  |  Direct Link  |  

5:  Hoareau, L. and E.J. DaSilva, 1999. Medicinal plants: A re-emerging health aid. Electron. J. Biotechnol., 2: 56-70.
Direct Link  |  

6:  Iwalewa, E.O., L.J. McGaw, V. Naidoo and J.N. Eloff, 2007. Inflammation: The foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. Afr. J. Biotechnol., 6: 2868-2885.
Direct Link  |  

7:  Malterud, K.E. and K.M. Rydland, 2000. Inhibitors of 15-lipoxygenase from orange peel. J. Agric. Food Chem., 48: 5576-5580.
CrossRef  |  

8:  McGaw, L.J., N. Lall, J.J. Meyer, J.N. Eloff, 2008. The potential of South African plants against Mycobacterium infections. J. Ethnopharmacol., 119: 482-500.
CrossRef  |  

9:  Moodley, N., N.R. Crouch, D.A. Mulholland, D. Slade and D. Ferreira, 2006. 3-Benzyl-4-chromanones (homoisoflavanones) from bulbs of the ethnomedicinal geophyte Ledebouria revoluta (Hyacinthaceae). S. Afr. J. Bot., 72: 517-520.
CrossRef  |  

10:  Ncube, N.S., A.J. Afolayan and A.I. Okoh, 2008. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. Afr. J. Biotechnol., 7: 1797-1806.
Direct Link  |  

11:  Osbourn, A., 1996. Saponins and plant defence: A soap story. Trends Plant Sci., 1: 4-9.
CrossRef  |  

12:  Rackova, L., M. Oblozinsky, D. Kostalova, V. Kettmann and L. Bezakova, 2007. Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. J. Inflamm., Vol. 4. 10.1186/1476-9255-4-15

13:  Taylor, J.L.S. and J. Van Staden, 2001. The effect of age, season and growth conditions on anti-inflammatory activity in Eucomis autumnalis (Mill.) Chitt. Plant extracts. Plant Growth Regul., 34: 39-47.
CrossRef  |  

14:  Watt, J.M. and M.G. Breyer-Brandwijk, 1962. The Medicinal and Poisonous Plants of Southern and Eastern Africa. 2nd Edn., E and S Liningstone Ltd., London, UK., Pages: 1457.

©  2020 Science Alert. All Rights Reserved