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Research Article
 

Extraction and Isolation Phytochemical and Antimicrobial Activity of Limonoid Compounds from Orange Waste Juice



Ucop Haroen, Yetti Marlida, Mirzah and Agus Budianyah
 
ABSTRACT

Antibacterial activity limonoid compounds of three different solvent extracts (methanol, ethyl acetate and n-heksane) prepared by soxhlet extractor from orange waste juice were screened against two pathogenic bacteria Escherichia coli and Salmonella enteridis. The highest antibacterial potentiality was exhibited by the limonoid-ethyl acetate followed by the (limonoid-methanol) and (limonoid-n-hexane).The orange waste juice extract can be considered to be as equally potent as the antibiotics. The zone of inhibition, Minimum Inhibitory Concentration (MIC) of the orange waste juice extracts showed that limonoids extracted with ethyl acetate (limonoids-ethyl acetate) resulted in better inhibition of limonoids extracted with (limonoids-methanol) and (limonoids-n-hexane). The concentration 250 ppm of MIC were test showed inhibition of (limonoids-ethyl acetate) higher with a percentage minimum (44.15 for the bacterium Escherichia coli and 14.69 for the salmonella enteridis) than (limonoids-methanol) (10.67 and 3.84) and (limonoids-n-hexane) (35.58 and 14.10). In vitro antibacterial screening of crude (limonoid-ethyl acetate) (limonoid-methanol) and (limonoid-n-hexane) of methanol extract of orange juice waste with concentration 250 ppm were test showed the clear zone in millimeters (11.75 for the bacterium Escherichia coli and 10.25 for the salmonella enteridis) than limonoid-methanol (8.00 and 9.00) and limonoid-n-hexane (8.00 and 9.25). However, when compared to synthetic antibiotics (neomiditril and coleridin), the clear zone of limonoids lower (24%) but still showed his ability as an anti-microbial. (Limonoids-ethyl acetate) dose of 250 ppm showed better activity than doses of (limonoid-methanol) and (limonoid-n-heksana) for the bacterium Escherichia coli to salmonella enteridis bacterial at 11.918 and 9.158. From these results we can conclude (limonoids-ethyl acetate) extract better than (limonoids-methanol) and (limonoids-n-hexane) and can be used as a substitute for anti biotic synthetic antimicrobial. The phytochemical analysis of orange waste juice extracts showed presence of triter penes, alkaloid, flavonoids, steroid, phenolic, saponin.

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  How to cite this article:

Ucop Haroen, Yetti Marlida, Mirzah and Agus Budianyah, 2013. Extraction and Isolation Phytochemical and Antimicrobial Activity of Limonoid Compounds from Orange Waste Juice. Pakistan Journal of Nutrition, 12: 730-735.

DOI: 10.3923/pjn.2013.730.735

URL: https://scialert.net/abstract/?doi=pjn.2013.730.735

REFERENCES
Ahmad, M.M., Salim-ur-Rehman, Z. Iqbal, F.M. Iqbal-Anjum and J.I. Sultan, 2006. Genetic variability to essential oil composition in four citrus fruit species. Pak. J. Bot., 38: 319-324.
Direct Link  |  

Akinmoladun, A.C., E.O. Ibukun, E. Afor, E.M. Obuotor and E.O. Farombi, 2007. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Scient. Res. Essay, 2: 163-166.
Direct Link  |  

Arbain, D., 1995. Phytochemical survey one way approach. Heds-Usaid Project Unand.

Caccioni, D.R.L., M. Guizzardi, D.M. Biondi, A. Renda and G. Ruberto, 1998. Relationship between volatile components of citrus fruit essential oils and antimicrobial action on Penicillium digitatum and Penicillium italicum. Int. J. Food Microbiol., 43: 73-79.
CrossRef  |  Direct Link  |  

Di Majo, D., M. Giammanco, M. la Guardia, E. Tripoli, S. Giammanco and E. Finotti, 2005. Flavanones in Citrus fruit: Structure-antioxidant activity relationships. Food Res. Int., 38: 1161-1166.
CrossRef  |  

Ekwenye, U.N. and O.V. Edeha, 2010. The antibacterial activity of crude leaf extract of Citrus sinensis (Sweet orange). Int. J. Pharma Bio Sci., 1: 742-750.
Direct Link  |  

FAO, 2004. FAOSTAT Data 2005. Food and Agriculture Organization of the United Nation, Rome, Italy.

Ferguson, J.J., 2002. Medicinal use of citrus. Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. October, 2002.

Friedman, M., P.R. Henika and R.E. Mandrell, 2002. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica. J. Food Prot., 65: 1545-1560.
PubMed  |  Direct Link  |  

Guthrie, N., S. Hasegawa, G. Manners and T.A. Vandenberg, 2000. Inhibition of Human Breast Cancer Cells by Citrus Limonoids. In: Citrus Limonoids: Functional Chemicals in Agriculture and Foods, Berhow, M., S. Hasegawa and G. Manners (Eds.). American Chemical Society, United States, pp: 164-174.

Ibekwe, V.I., N.F. Nnayere and C.O. Akujobi, 2001. Studies on antibacterial activity and phytochemical qualities of extracts of orange peels. Int. J. Environ. Health Hum. Dev., 2: 41-46.
Direct Link  |  

Kirbaslar, F.G., A. Tavman, B. Dulger and G. Turker, 2009. Antimicrobial activity of Turkish Citrus peel oils. Pak. J. Bot., 41: 3207-3212.
Direct Link  |  

Ko, H.C., Y.H. Wang, K.T. Liou, C.M. Chen and C.H. Chen et al., 2007. Anti-inflammatory effects and mechanisms of the ethanol extract of Evodia rutaecarpa and its bioactive components on neutrophils and microglial cells. Eur. J. Pharmacol., 555: 211-217.
CrossRef  |  

Kumamoto, H., Y. Matsubara, Y. Iizuka, K. Okamoto and K. Yokoi, 1986. Structure and hypotensive effect of flavonoid glycosides in orange (Citrus sinensis Osbeck) peelings. Agric. Biol. Chem., 50: 781-783.
Direct Link  |  

Kumar, K.A., M. Narayani, A. Subanthini and M. Jayakumar, 2011. Antimicrobial activity and phytochemical analysis of citrus fruit peels-utilization of fruit waste. Int. J. Eng. Sci. Tech., 3: 5414-5421.
Direct Link  |  

Lindsey, K., A.K. Jager, D.M. Raidoo and J. van Staden, 1999. Screening of plants used by Southern African traditional healers in the treatment of dysmenorrhoea for prostaglandin-synthesis inhibitors and uterine relaxing activity. J. Ethnopharmacol., 64: 9-14.
CrossRef  |  PubMed  |  

Manthey, J.A. and K. Grohmann, 2001. Phenols in citrus peel byproducts. Concentrations of hydroxycinnamates and polymethoxylated flavones in citrus peel molasses. J. Agric. Food Chem., 49: 3268-3273.
CrossRef  |  

Miller, E.G., J.L. Porter, W.H. Binnie, I.Y. Guo and S. Hasegawa, 2004. Correction to profile of plasma and urine metabolites after the intake of almond [Prunus dulcis (Mill.) D.A. Webb] polyphenols in humans. J. Agric. Food Chem., 52: 4908-4912.
CrossRef  |  

Nand, K., 1998. Recent advances in the treatment of liquid and solid wastes of food processing industries for biogas production and pollution abatenront. Proceedings of the 4th International Food Convention, November 23-27, 1998, Mysore, India, pp: 35-.

Nychas, G.J.E. and C.C. Tassou, 2000. Traditional Preservative Oils and Spices. In: Encyclopedia of Food Microbiology, Robinson, R.K., C.A. Batt and P.D. Patel (Eds.). Vol. 2, Academy Press, London.

Poulose, S.M., E.D. Harris and B.S. Patil, 2005. Citrus limonoids induce apoptosis in human neuroblastoma cells and have radical scavenging activity. J. Nutr., 135: 870-877.
Direct Link  |  

Roy, A. and S. Saraf, 2006. Limonoids: Overview of significant bioactive triterpenes distributed in plants kingdom. Biol. Pharmaceut. Bull., 29: 191-201.
Direct Link  |  

Singh, A., A.R. Bilal and A. Devgun, 2009. In vitro antibiotic activity of isolated volatile oil of Citrus sinensis. Mater. Methods, 7: 1-4.

Sokovic, M., P.D. Marin, D. Brkic and L.J.L.D. van Griensven, 2007. Chemical composition and antibacterial activity of essential oils of ten aromatic plants against human pathogenic bacteria. http://www.baltikjunior.com/origano_doc/sinisa_stankovic_1.pdf.

Voogd, C.E., 1981. On the mutagenicity of nitroimidazoles. Mutation Res./Rev. Genet. Toxicol., 86: 243-277.
CrossRef  |  Direct Link  |  

Wilson, C.L. and G.G. Droby, 2000. Microbial Food Contamination. CRC Press, Boca Raton, FL., pp: 149-171.

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