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Research Article
 

Evaluation of Phytochemical Composition and Antimicrobial Activity of Sweet Potato (Ipomoea batatas) Leaf



I.E. Mbaeyi-Nwaoha and V.N. Emejulu
 
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ABSTRACT

Sweet Potato Leave (SPL) powder and its peptone, ethanol and water extracts were subjected to proximate, phytochemical, anti-nutrient and antimicrobial analysis. The results of proximate analysis revealed the presence of high carbohydrate (43.971±0.019-56.472±0.009%) and crude protein (16.497±0.003-28.163±0.003%). The sweet potato leaves powder and the extracts also contain moisture (3.603±0.004-16.921±0.008%), ash (5.563±0.004-10.414±0.007%), fat (0.208±0.007-14.264±0.002%) and fibre (0.244±0.003-2.764±0.003%), respectively. The phytochemical screening revealed the presence of bioactive compounds in the SPL powder and its extracts namely; tannins, alkaloids, steroids, glycosides, saponins, flavonoids and soluble carbohydrates. The SPL powder had the highest content of these bioactive compounds with alkaloid having the highest value of 3.784±0.007mg/100g, followed by flavonoid (3.357±0.006mg/100g) and steroid had the lowest value (0.393±0.003mg/100g). The extracts had highest content of flavonoid ranging between 2.762±0.008 and 3.355±0.004 mg/100g and least content of steroid ranging between 0.357±0.002 and 0.375±0.003mg/100g. The anti-nutrient analysis indicated the content of very high amount of oxalate ranging between 1.664±0.004 and 6.254±0.004% in the four samples. They contained lower amounts of phytate (3.897±0.003 to 5.933±0.003mg/100g), cyanide (0.353±0.003 to 1.444±0.004mg/100g) and tannin (5.527±0.002 to 9.010±0.002mg/100g). The antimicrobial activity of the peptone, water and ethanol extracts was tested against Escherichia coli, Salmonella typhi, Staphylococcus aureus, Aspergillus niger, Penicillium spp., Pseudomonas aeroginosa and Klebsiella pneumonia. The water extract showed the best antimicrobial activity by inhibiting the growth of all the organisms except E. coli and Penicillium spp at different concentrations of the extract. While the ethanol and peptone extracts only had a minimal activity against Salmonella typhi.

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  How to cite this article:

I.E. Mbaeyi-Nwaoha and V.N. Emejulu, 2013. Evaluation of Phytochemical Composition and Antimicrobial Activity of Sweet Potato (Ipomoea batatas) Leaf. Pakistan Journal of Nutrition, 12: 575-586.

DOI: 10.3923/pjn.2013.575.586

URL: https://scialert.net/abstract/?doi=pjn.2013.575.586

REFERENCES
1:  Aja, P.M., A.N.C. Okaka, P.N. Onu, U. Ibiam and A.J. Urako, 2010. Phytochemical composition of Talinum triangulare (water leaf) leaves. Pak. J. Nutr., 9: 527-530.
CrossRef  |  Direct Link  |  

2:  AOAC., 2010. Official Methods of Analysis. 15th Edn., Association of Official Analytical Chemists, Washington, DC., USA.

3:  Anon, 2011. A visual guide to yams and sweet potatoes (Plus how they fit into a primary eating plan). http://www.marksdailyapple.com/difference-yams-sweet-potatoes/#axzz2icvDPvYm

4:  Anowi, C.F., N.C. Cardinal, C.J. Mbah and T.C. Onyekaba, 2012. Antimicrobial properties of the methanolic extract of the leaves of Nauclea latifolia. Int. J. Drug Res. Technol., 2: 45-55.
Direct Link  |  

5:  Antia, B.S., E.J. Akpan, P.A. Okon and I.U. Umoren, 2006. Nutritive and anti-nutritive evaluation of sweet potatoes (Ipomoea batatas) leaves. Pak. J. Nutr., 5: 166-168.
CrossRef  |  Direct Link  |  

6:  Aregheore, E.M., 2012. Nutritive value and inherent anti-nutritive factors in four indigenous edible leafy vegetables in human nutrition in Nigeria: A review. J. Food Resour. Sci., 1: 1-14.
CrossRef  |  

7:  Bamishaiye, E.I., F.F. Olayemi, E.F. Awagu and O.M. Bamshaiye, 2011. Proximate and phytochemical composition of Moringa oleifera leaves at three stages of maturation. Adv. J. Food Sci. Technol., 3: 233-237.
Direct Link  |  

8:  CARD, 2010. Commercial and high quality cultivars of root and tuber crops for processing purpose in the northern and central vietnam. Ministry of Agriculture and Rural Development, Vietnam.

9:  Cowan, M.M., 1999. Plant products and antimicrobial agents. Clin. Microbiol. Rev., 12: 564-582.
PubMed  |  Direct Link  |  

10:  Duke, J.A., 1998. Ipomoea batatas (L.) lam. Purdue University.

11:  Etuk, E.U., M.N. Bassey, U.O. Umoh and E.G. Inyang, 1998. Comparative nutritional studies on three Local varieties of Heinsia crinita. Plant Varieties Seeds, 11: 151-158.
Direct Link  |  

12:  Fasuyi, A.O., 2006. Nutritional potentials of some tropical vegetable leaf meals: Chemical characterization and functional properties. Afr. J. Biotechnol., 5: 49-53.
Direct Link  |  

13:  Harborne, J.B., 1973. Phytochemical Method of Analysis. Chapman and Hall Ltd., London.

14:  Ishiguro, K., J. Toyama, S. Islam, M. Yoshimoto and T. Kumagai et al., 2004. Suioh, a new sweetpotato cultivar for utilization in vegetable greens. Acta Horticult., 637: 339-345.

15:  Islam, S., M. Jalaluddin, M. Yoshimoto and O. Yamakawa, 2004. Growth inhibition of pathogenic bacteria by artificially grown Ipomoea batatas (L.) leaf. Hort. Sci., 39: 778-778.

16:  Kemper Center for Home Gardening, 2009.. Ipomoea batatas. Missouri Botanical Garden. http://www.mobot.org/gardeninghelp/plantfinder/plant.asp?code=A587#lbl_culture.

17:  Low, J., R. Kapinga, D. Cole, C. Loechl, J. Lynam and M. Andrade, 2009. Unleashing potential of sweet potato in sub-saharan Africa: Nutritional impact with orange-fleshed sweet potato (OFSP). Social Sci. Working Paper, 1: 73-104.

18:  Oboh, G., 2006. Nutritive value and haemolytic properties In vitro of the leaves of Vernonia amygdalina on human erythrocyte. Nutr. Health, 18: 151-160.
PubMed  |  

19:  Ogbe, A.O. and J.P. Affiku, 2011. Proximate study, mineral and anti-nutrient composition of Moringa oleifera leaves harvested from Lafia, Nigeria: Potential benefits in poultry nutrition and health. J. Microbiol. Biotechnol. Food Sci., 1: 296-308.
Direct Link  |  

20:  Oloyede, O.I., 2005. Chemical profile of unripe pulp of Carica papaya. Pak. J. Nutr., 4: 379-381.
CrossRef  |  Direct Link  |  

21:  Onwordi, C.T., A.M. Ogungbade and A.D. Wusu, 2009. The proximate and mineral composition of three leafy vegetables commonly consumed in Lagos, Nigeria. Afr. J. Pure Applied Chem., 3: 102-107.
Direct Link  |  

22:  Onwuka, G.I., 2005. Food Analysis: Theory and Practice. Naphthali Prints, Lagos, Nigeria, pp: 133-199.

23:  Onyeka, E.U. and I.O. Nwambekwe, 2007. Phytochemical profile of some green leafy vegetables in South East, Nigeria. Niger. Food J., 25: 67-76.
Direct Link  |  

24:  Oyenuga, V.A., 1968. Nigerian Foods and Feeding Stuffs, their Chemistry and Nutritve Value. Ibadan University Press, Ibadan, pp: 23-27.

25:  Pearson, D., 1978. The Chemical Analysis of Foods. 7th Edn., Churchill Livingstone, Edinburgh, pp: 494-497.

26:  Pochapski, M.T., E.C. Fosquiera, L.A. Esmerino, E.B. Dos Santos, P.V. Farago, F.A. Santos and F.C. Groppo, 2011. Phytochemical screening, antioxidant and antimicrobial activities of the crude leaves' extract from Ipomoea batatas (L.) lam. Pharmacogn. Mag., 7: 165-170.
CrossRef  |  PubMed  |  Direct Link  |  

27:  Schronk, M.J., 2010. The sweet potato: An important part of human health. Department of Horticulture, Texas A and M University, College Station, TX 77843.

28:  Tijjani, M.B., I.J. Bello, A.B. Aliyu, T. Olurishe, S.M. Maidawa, J.D. Habila and E.O. Balogun, 2009. Phytochemical and antimicrobial studies of root extract of Cochlospermum tinctorium A. rich. (Cochlospermaceae). Res. J. Med. Plants, 3: 16-22.
CrossRef  |  Direct Link  |  

29:  Wan, J., A. Wilcock and M.J. Conventry, 1998. Comparative evaluation of methods commonly used to determine antimicrobial susceptibility to plant extracts and phenolic compounds. J. Applied Microbiol., 84: 152-158.

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