Subscribe Now Subscribe Today
Research Article
 

Effect of Dietary Vegetable Oil Supplementation on C18 Fatty Acids and Conjugated Linoleic Acid Production; An In vitro Fermentation Study



Julakorn Panatuk, Suthipong Uriyapongson, Chainarong Nawanukraw, Chirasak Phoemchalard and Pitukpol Pornanake
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

An in vitro rumen fermentation trial was conducted to investigate effect of dietary vegetable oil supplement on fatty acids metabolism in cattle. The incubation was carried out using rumen fluid obtained from Brahman x Native crossbred beef cattle. The diets were formulated by using basal diet with no vegetable oil (CON), basal diet with 4% of palm oil (PO), 4% of canola oil (CA), 4% of coconut oil (CO) and 4% of sunflower oil (SF). Fatty acid profiles were determined at 12, 24 and 48 h after incubation time. The results found that linoleic acid (LA) was decreased with diet CON, diet PO, diet CA and diet SF (p<0.05). Stearic acid (SA) resulted increased with diet CON, diet CA, diet CO and diet SF at 24 and 48 h. The SF diet contain high level of LA showed the highest concentration of cis9 trans11 conjugated linoleic acid (CLA) when compared with other diets. Vaccenic acid (VA) was increased with all the five diet, especially with diet SF and diet CA after 24 h. Base on this study, it suggests that supplementation of SF could improve the concentration of VA and cis9 trans11 CLA in the rumen.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Julakorn Panatuk, Suthipong Uriyapongson, Chainarong Nawanukraw, Chirasak Phoemchalard and Pitukpol Pornanake, 2013. Effect of Dietary Vegetable Oil Supplementation on C18 Fatty Acids and Conjugated Linoleic Acid Production; An In vitro Fermentation Study. Pakistan Journal of Nutrition, 12: 516-520.

DOI: 10.3923/pjn.2013.516.520

URL: https://scialert.net/abstract/?doi=pjn.2013.516.520

REFERENCES
1:  AOAC, 1995. Official Method of Analysis, Animal Feeds. 16th Edn., Association of Official Analytical Chemists, Virginia, USA., pp: 1-18.

2:  AbuGhazaleh, A.A., D.J. Schingoethe, A.R. Hippen and K.F. Kalscheur, 2003. Conjugated linoleic acid and vaccenic acid in rumen, plasma and milk of cows fed fish oil and fats differing in saturation of 18 carbon fatty acids. J. Dairy Sci., 86: 3648-3660.

3:  Buccioni, A., M. Antongiovanni, F. Petacchi, M. Mele, A. Serra, P. Secchiari and D. Benvenuti, 2006. Effect of dietary fat quality on C 18: 1 fatty acids and conjugated linoleic acid production: An in vitro rumen fermentation study. Anim. Feed Sci. Technol., 127: 268-282.
CrossRef  |  

4:  Bauman, D.E., B.A. Corl and D.J. Peterson, 2003. The Biology of Conjugated Linoleic Acids in Ruminants. In: Advances in Conjugated Linoleic Acid Research, Sebedio, J.L., W.W. Christie and R.O. Adlof (Eds.). AOCS Press, Champaign, IL. USA., pp: 146-173.

5:  Beam, T.M., T.C. Jenkins, P.J. Moate, R.A. Kohn and D.L. Palmquist, 2000. Effects of amount and source of fat on the rates of lipolysis and biohydrogenation of fatty acids in ruminal contents. J. Dairy Sci., 83: 2564-2573.
CrossRef  |  Direct Link  |  

6:  Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
PubMed  |  Direct Link  |  

7:  Griinari, J.M. and D.E. Bauman, 1999. Biosynthesis of Conjugated Linoleic Acid and its Incorporation into Meat and Milk in Ruminants. In: Advances in Conjugated Linoleic Acid Research, Yurawecz, M.P., M.M. Mossoba, J.K.G. Kramer, G. Nelson and M.W. Pariza (Eds.). Vol. 1, AOCS Press, Champaign, IL., pp: 180-199.

8:  Jalc, D., A. Potkanski, M. Szumacher-Strabel, A. Cieslak and M. Certik, 2005. Effect of microbial oil, evening primrose oil and borage oil on rumen fermentation in vitro. Veterinarni Medicina Czech, 50: 480-483.
Direct Link  |  

9:  Jenkins, T.C., 1993. Lipid metabolism in the rumen. J. Dairy Sci., 76: 3851-3863.
CrossRef  |  Direct Link  |  

10:  Khanal, R.C. and T.R. Dhiman, 2004. Biosynthesis of conjugated linoleic acid (CLA): A review. Pak. J. Nutr., 3: 72-81.
CrossRef  |  Direct Link  |  

11:  Kramer, J.K.G., N. Sehat, J. Fritsche, M.M. Mossoba, K. Eulitz, M.P. Yurawecz and Y. Ku, 1999. Seperation of Conjugated Linoleic acid Isomers. In: Advances in Conjugated Linoleic Acid Research, Yurawecz, M.P., M.M. Mossoba, J.K.G. Kramer, M.W. Pariza and G.J. Nelson (Eds.). Vol. 1, AOCS Press, Champaign, IL., USA., pp: 81-109.

12:  Loor, J.J., A.B.P.A. Bandara and J.H. Herbein, 2002. Characterization of C18:1 and C18:2 isomers produced during microbial biohydrogenation of unsaturated fatty acid from canola and soya bean oil in the rumen of lactating cows. J. Anim. Physiol. Nutr., 86: 422-432.

13:  Martin, S.A. and T.C. Jenkins, 2002. Factors affecting conjugated linoleic acid and trans-C18:1 fatty acid production by mixed ruminal bacteria. J. Anim. Sci., 80: 3347-3352.
Direct Link  |  

14:  Matsushita, M., N.M. Tazinafo, R.G. Padre, C.C. Oliveira and N.E. Souza et al., 2007. Fatty acid profile of milk from Saanen goats fed a diet enriched with three vegetable oils. Small Rumin. Res., 72: 127-132.
CrossRef  |  

15:  Menke, K.H. and H. Steingass, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 28: 7-55.
Direct Link  |  

16:  Metcalfe, L.D., A.A. Schmitz and J.R. Pelka, 1966. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal. Chem., 38: 514-515.
CrossRef  |  Direct Link  |  

17:  Mosley, E.E., G.L.Powell, M.B. Riley and T.C. Jenkins, 2002. Microbial biohydrogenation of oleic acid to Trans isomers In vitro. J. Lipid Res., 43: 290-296.
Direct Link  |  

18:  NRC., 2001. Nutrient Requirements of Dairy Cattle. 7th Edn., National Academy of Sciences, Washington, DC., USA.

19:  Panyakaew, P., G. Goel, M. Lourenco, C. Yuangklang and V. Fievez, 2013. Medium-chain fatty acids from coconut or krabok oil inhibit in vitro rumen methanogenesis and conversion of non-conjugated dienoic biohydrogenation intermediates. Anim. Feed Sci. Technol., 180: 18-25.
CrossRef  |  

20:  Selner, D.R. and L.H. Schultz, 1980. Effects of feeding oleic acid or hydrogenated vegetable oils to lactating cows. J. Dairy Sci., 63: 1235-1241.
CrossRef  |  

21:  Steel, R.G.D., J.H. Torrie and D.A. Deekey, 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edn., Mc Graw Hill, New York, ISBN: 9780070610286, pp: 400-428.

22:  Szumacher-Strabel, M., A. Cieslak and A. Nowakowska, 2009. Effect of oils rich in linoleic acid on in vitro rumen fermentation parameters of sheep, goats and dairy cows. J. Anim. Feed Sci., 18: 440-452.
Direct Link  |  

23:  Troegeler-Meynadier, A., M.C. Nicot, C. Bayourthe, R. Moncoulon and F. Enjalbert, 2003. Effects of pH and concentrations of linoleic and linolenic acids on extent and intermediates of ruminal biohydrogenation in vitro. J. Dairy Sci., 86: 4054-4063.
CrossRef  |  

24:  Van Soest, P.J., J.B. Robertson and B.A. Lewis, 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.
CrossRef  |  PubMed  |  Direct Link  |  

25:  Wolff, R.L. and C.C. Bayard, 1995. Improvement in the resolution of individualtrans-18: 1 isomers by capillary gas-liquid chromatography: Use of a 100-m CP-Sil 88 column. J. Am. Oil Chemists Soc., 72: 1197-1201.
CrossRef  |  

©  2021 Science Alert. All Rights Reserved