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Research Article

Variation in the Levels of B-Vitamins and Protein Content in Wheat Flours

Madiha Rohi, Imran Pasha, Masood Sadiq Butt and Haq Nawaz
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Wheat is a vital crop having imperative nutrients for human diet e.g., protein and certain B-vitamins (thiamin, riboflavin, niacin and pyridoxine), well-intentioned for body metabolic processes. These vitamins are concentrated in grain outer layer thus milling process affects these vitamins to a larger extent and may reduce their level. The aim of the present study is to determine the level of protein and B-vitamins in whole wheat and straight grade flours of different spring wheats. Ten spring wheat varieties were procured from different parts of the country, milled and quantified. The protein content was determined by Kjeldhal’s method and B-vitamins were analyzed through High Performance Liquid Chromatography (HPLC) using UV-VIS detector. Results revealed that the level of protein as well as the B-vitamins was high in whole wheat flours of spring wheats as compared to straight grade flour. Only 3, 15, 6 and 6% of thiamin, riboflavin, niacin and pyridoxine of whole wheat flour was detected in straight grade flour. Simple Pearson’s correlation has been studied among wheat B-vitamins and its protein content which exhibited that in whole wheat flour a positive correlation for protein content with thiamin (r = 0.54), riboflavin (r = 0.07) and niacin (r = 0.22) whereas in straight grade flour protein concentration was examined to be positively correlated with riboflavin concentration (r = 0.19) only. Conclusively, the results of the current study are useful for different stake holders for their intended uses.

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  How to cite this article:

Madiha Rohi, Imran Pasha, Masood Sadiq Butt and Haq Nawaz, 2013. Variation in the Levels of B-Vitamins and Protein Content in Wheat Flours. Pakistan Journal of Nutrition, 12: 441-447.

DOI: 10.3923/pjn.2013.441.447



1:  AACC., 2000. Approved Methods of American Association of Cereal Chemists. 10th Edn., American Association of Cereal Chemists, Minnesota, USA

2:  Adachi, T., K. Watanabe and T. Mitsunaga, 2000. Characterization of thiamine binding protein from wheat germ. Cereal Chem., 77: 578-581.
CrossRef  |  

3:  Adrian, J. and L. Petit, 1970. Vitamins of cereals and their evolution during technologic treatment. Ann. de Nutr. Aliment, 24: 131-168.

4:  Ahmad, I., 2001. Varietal difference in amino acid composition, milling and baking properties of spring wheats. Ph.D. Thesis, National Institute of Food Science and Technology, University of Agriculture Faisalabad, Pakistan.

5:  Akhtar, S., F.M. Anjum and S.U. Rehman, 2005. Effect of iron and zinc fortification on chemical composition of whole wheat flour. J. Res. Sci., 16: 95-103.
Direct Link  |  

6:  Aslam, J., M.S. Mohajir, S.A. Khan and A.Q. Khan, 2008. HPLC analysis of water-soluble vitamins (B1, B2, B3, B5, B6) in in vitro and ex vitro germinated chickpea (Cicer arietinum L.). Afr. J. Biotechnol., 7: 2310-2314.

7:  Batifoulier, F., M.A. Verny, E. Chanliaud, C. Remesy and C. Demigne, 2005. Effect of different breadmaking methods on thiamine, riboflavin and pyridoxine contents of wheat bread. J. Cereal Sci., 42: 101-108.
CrossRef  |  

8:  Batifoulier, F., M.A. Verny, E. Chanliaud, C. Remesy and C. Demigne, 2006. Variability of B vitamin concentrations in wheat grain, milling fractions and bread products. Eur. J. Agronomy, 25: 163-169.
CrossRef  |  

9:  Beaugrand, J., D. Cronier, P. Thiebeau, L. Schreiber, P. Debeire and B. Chabbert, 2004. Structure, chemical composition and xylanase degradation of external layers isolated from developing wheat grains. J. Agric. Food Chem., 52: 7108-7117.
PubMed  |  

10:  Bertrais, S., M.L. Polo-Luque, P. Preziosi, B. Fieux, M. Torra-De-Flot, P. Galan and S. Hercberg, 2000. Contribution of ready-to-eat cereals to nutrition intakes in French adults and relations with corpulence. Ann. Nutr. Metab., 44: 249-255.
PubMed  |  

11:  Butt, M.S., F.M. Anjum, A. Rehman and A. Ali, 1997. Physico-chemical characteristics of spring wheats. J. Agric. Res., 35: 413-422.

12:  GOP, 2012. Economic survey of Pakistan. Economic Affairs Division, Ministry of Finance, Islamabad, Pakistan.

13:  Hercberg, S., P. Preziosi, P. Galan, M. Devanlay and H. Keller et al., 1994. Vitamin status of a healthy French population: Dietary intakes and biochemical markers. Int. J. Vitamin Nutr. Res., 64: 220-232.
PubMed  |  

14:  Hussain, A., H. Larsson, M.E. Olsson, R. Kuktaite, H. Grausgruber and E. Johansson, 2012. Is organically produced wheat a source of tocopherols and tocotrienols for health food? Food Chem., 132: 1789-1795.
CrossRef  |  

15:  Ikhtiar, K. and Z. Alam, 2007. Nutritional composition of Pakistani wheat varieties. J. Zhejiang Univ. Sci. B., 8: 555-559.
PubMed  |  

16:  Indrani, D., P. Swetha, C. Soumya, J. Rajiv and G.V. Rao, 2011. Effect of multigrains on rheological, microstructural and quality characteristics of north Indian parottas-An Indian flat bread. LWT-Food Sci. Technol., 44: 719-724.
CrossRef  |  

17:  Jenkins, D.J., C.W. Kendall, T.H. Nguyen, A. Marchie and D.A. Faulkner et al., 2008. Effect of plant sterols in combination with other cholesterol-lowering foods. Metabolism-Clin. Exp., 57: 130-139.
CrossRef  |  

18:  Kaldy, M.S. and G.L. Rubenthaler, 1987. Milling, baking and physical-chemical properties of selected soft white winter and spring wheat. Cereal Chem., 64: 302-307.
Direct Link  |  

19:  Keagy, P.M., B. Borenstein, P. Ranum, M.A. Connor and K. Lorenz et al., 1980. Natural levels of nutrients in commercially milled wheat ours. II. Vitamin analysis. Cereal Chem., 57: 59-65.
Direct Link  |  

20:  Lebiedzinska, A. and P. Szefer, 2006. Vitamins B in grain and cereal-grain food, soy-products and seeds. Food Chem., 95: 116-122.
CrossRef  |  

21:  MacMasters, M.M., J.J.C. Hinton and D. Bradbyru, 1971. Microscopic Structure and Composition of Wheat Kernel. In: Wheat: Chemistry and Technology, Pomeranz, Y.E. (Ed.). American Association of Cereal Chemists, USA., pp: 51
CrossRef  |  

22:  OECD, 2003. Consensus document on compositional considerations for new varieties of bread wheat (ttriticum aestivum): key food and feed nutrients. Anti-Nutrients and Toxicants, Paris, Cedex 16 France.

23:  Orth, R.A. and W.A. Bushuk, 1972. A comparative study of the proteins of wheats of diverse baking qualities. Cereal Chem., 49: 268-275.

24:  Payne, P.I., M.A. Nightingale, A.F. Krttiger and L.M. Holt, 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food Agric., 40: 51-65.
CrossRef  |  Direct Link  |  

25:  Pederson, B., K.E.B. Knudsen and E.O. Eggum, 1989. Nutritional Value of Cereal Products, Beans and Starches. In: World Review of Nutrition and Dietetics, Bourne, G.H. (Ed.). Karger, Basel, pp: 2-76

26:  Piironen, V., A.M. Lampi and P. Ekholm, 2006. Micronutrients and Phytochemicals in Wheat Grain. In: Wheat Chemistry and Technology, Khan, K. and P.R. Shewry (Eds.). 4th Edn., Am. Assoc. Cereal Chemists, USA., pp: 179-185

27:  Pomeranz, Y., 1988. Chemical Composition of Kernel Structures. In: Wheat: Chemistry and Technology, Pomeranz, Y. (Ed.). Vol. 1, 3rd Edn., AACC International, St. Paul, MN., pp: 97- 158

28:  Rivlin, R.S. and J.T. Pinto, 2001. Riboflavin. In: The Handbook of Vitamins, Ruker, R.B. and J.W. Suttie (Eds.). 3rd Edn., Dekker, New York, pp: 255-273

29:  Roth-Maier, D.A., S.I. Wild, W. Erhardt, J. Henke and M. Kirchgessner, 1999. Investigations on the intestinal availability of native thiamin in selected foods and feedstuffs. Eur. J. Clin. Nutr., 38: 241-246.
PubMed  |  

30:  Saeed, F., 2012. Biochemical characterization of non starch polysaccharides in relation to end use quality of spring wheats. Ph.D Thesis, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.

31:  Serna-Saldiver, S.O., 2005. Chemical Composition of Cereals Grains. In: Cereal Grains Properties, Processing and Nutritional Distribution, Barbosa-Canovas, G.V. (Ed.). CRC Press, Boca Raton, London, pp: 81-90

32:  Singh, J., P.J. Sharp and J.H. Skerritt, 2000. A new candidate protein for high lysine content in wheat grain. J. Sci. Food Agric., 81: 216-226.
CrossRef  |  

33:  Slaughter, D.C., K.H. Norris and W.R. Hruschka, 1992. Quality and classification of hard red wheat. Cereal Chem., 69: 428-432.
Direct Link  |  

34:  Sliwinski, E.L., P. Kolster, P., A. Prins and T. Van Vliet, 2004. On the relationship between gluten protein composition of wheat flours and large-deformation properties of their doughs. J. Cereal Sci., 39: 247-264.
CrossRef  |  Direct Link  |  

35:  Steel, R.G.D., J.H. Torrie and D.A. Dickey, 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edn., McGraw-Hill Co., New York, USA., ISBN: 9780070610286, Pages: 666

36:  Tsukatani, T., H. Suenaga, M. Ishiyama, T. Ezoe and K. Matsumoto, 2011. Determination of water-soluble vitamins using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts. Food Chem., 127: 711-715.
CrossRef  |  

37:  Watanabe, K., M. Ampo, T. Ohtsuka, H. Takahashi and T. Mitsunaga, 2003. Synthesis of thiamine-binding protein during seed development and its accumulation in sesame seed. Plant Biotechnol., 20: 221-224.

38:  Watanabe, K., N. Nishida, T. Adachi, M. Ueda, T. Mitsunaga and Y. Kawamura, 2004. Accumulation and degradation of thiamine-binding protein and level of thiamine in wheat seeds during seed maturation and germination. Biosci. Biotechnol. Biochem., 68: 1243-1248.
PubMed  |  

39:  WHO, 2000. Human vitamins and mineral requirements: Report of a joint FAO/WHO expert consultation. Food and Nutrition Division, Bangkok, Thailand, pp: 27-32.

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