Oyewale Mojeed Oladapo
Department of Applied Sciences, Osun State Polytechnic, P.M.B. 301, Iree, Osun State, Nigeria
ABSTRACT
Aspergillus flavus exhibited high growth and amylase production. Various Polysaccharides, including soluble starch, sorghum, cassava peel and maize induces high amylase production with maximal amylase activity at 2% W/V. An initial linear increase in amylase activity with increase in substrate concentration was obtained up to 2% W/V. An antagonistic relationship was obtained between amylase activity and substrate concentrations higher than 2% W/V, in the culture filtrate of Aspergillus flavus grown in shake flasks. The maximum growth and amylase production was obtained on the sixth day of incubation at a pH of 7.0 temperature of 29±1°C and 80 rpm. It is concluded from this study that cassava peel as well as sorghum may be an alternative carbon substrate for large scale cultivation of A. flavus for amylase production.
PDF References
How to cite this article
Oyewale Mojeed Oladapo, 2013. Optimization of Growth and Amylase Production by Aspergillus flavus
Grown on Some Agricultural Raw Materials in Nigeria. Pakistan Journal of Nutrition, 12: 427-432.
DOI: 10.3923/pjn.2013.427.432
URL: https://scialert.net/abstract/?doi=pjn.2013.427.432
DOI: 10.3923/pjn.2013.427.432
URL: https://scialert.net/abstract/?doi=pjn.2013.427.432
REFERENCES
- Ali, S., S. Mahmood, R. Alam and Z. Hossain, 1989. Culture condition for production of glucoamylase from rice bran by Aspergillus terreus. MIRCEN J. Applied. Microbiol. Biotechnol., 5: 525-532.
CrossRef - Ettalibi, M. and J.C. Baratti, 1988. Isolation and characterization of an amylolytic yeast: Candida edax. MIRCEN. J. Applied Microbiol. Biotech., 6: 193-202.
CrossRef - Fergus, C.L., 1969. The production of amylase by some thermophilic fungi. Mycologia, 61: 1171-1175.
Direct Link - Garg, S.K. and H.H. Doelle, 1989. Optimization of cassava starch conversion to glucose by Rhizopus oligosporus. MIRCEN J. Applied Microbiol. Biotechnol., 5: 297-305.
CrossRef - Lagzouli, M., M. Zakaria, A. Ali, W. Senhaji and M. Ouhssine et al., 2007. Optimization of growth and extracellular glucoamylase production by Candida famata isolate. Afr. J. Biotechnol., 6: 2590-2595.
Direct Link - Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
CrossRefPubMedDirect Link - Mountfort, D.O. and R.A. Asher, 1988. Production of α-amylase by the ruminal anaerobic fungus (Neocaellimastix frontalis). Applied Environ. Microbiol., 54: 2293-2299.
PubMed - Ogundero, V.W., 1979. Amylase and cellulase activities of thermophilic fungi causing deterioration of Tobacco products in Nigeria. Mycopathologia, 69: 131-135.
CrossRef - Pestana, F. and F.J. Castillo, 1985. Glucoamylase production by Aspergillus awamori on rice flour medium and partial characterization of the enzyme. MIRECN J. Applied Microbiol. Biotechnol., 1: 225-237.
CrossRef