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Research Article

Total Phenolic Content and In vitro Antioxidant Activity of Winged Bean (Psophocarpus tetragonolobus)

R. Mohd Adzim Khalili, S. Emynur Shafekh, A.H. Norhayati, I. Mohd Fatahudin, R. Rahimah, H. Norkamaliah and A. Nor Azimah

Psophocarpus tetragonolobus also known as the Asparagus pea or winged pea (Family: Fabaceae) most widely grown in hot, humid equatorial countries like Malaysia. It provides a source of vitamin A, vitamin C, calcium and iron. The antioxidant potential of 75% methanol extract, ethyl acetate and chloroform soluble fractions of P. tetragonolobus was screened for in-vitro antioxidant activity using total phenolic content, ferric reducing power, 2, 2’-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay, Ferric Thiocyanate (FTC) and Thiobarbituric Acid (TBA) tests. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (1.69 mg/g GAE in concentration 0.5 mg/mL) higher than methanol and chloroform fractions. Antioxidant concentration was found highest in ethyl acetate extract (27.12 mM) using FRAP method, while chloroform extract has the highest antioxidant level (4.48 mM) using ABTS assay. Ethyl acetate extract of P. tetragonolobus was found to have highest antioxidant activity (7.27%) compared to methanol and chloroform using FTC method. While, TBA results shows no difference in antioxidant activity of all extract of P. tetragonolobus.

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  How to cite this article:

R. Mohd Adzim Khalili, S. Emynur Shafekh, A.H. Norhayati, I. Mohd Fatahudin, R. Rahimah, H. Norkamaliah and A. Nor Azimah, 2013. Total Phenolic Content and In vitro Antioxidant Activity of Winged Bean (Psophocarpus tetragonolobus). Pakistan Journal of Nutrition, 12: 416-422.

DOI: 10.3923/pjn.2013.416.422


Aris, S.R.S., S. Mustafa, N. Ahmat, F.M. Jaafar and R. Ahmad, 2009. Phenolic content and antioxidant activity of fruits of Ficus deltoidea var angustifolia sp. Malaysian J. Anal. Sci., 13: 146-150.
Direct Link  |  

Balasundram, N., K. Sundram and S. Samman, 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence and potential uses. Food Chem., 99: 191-203.
CrossRef  |  Direct Link  |  

Farag, R.S., A.Z.M.A. Badei, F.M. Hewedi and G.S.A. El-Baroty, 1989. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. J. Am. Oil Chem. Soc., 66: 792-799.
CrossRef  |  Direct Link  |  

Ganie, S.A., E. Haq, A. Masood, A. Hamid and M.A. Zargar, 2011. Antioxidant and protective effect of ethyl acetate extract of Podophyllum hexandrum rhizome on carbon tetrachloride induced rat liver injury. Evid. Based Complement Altern. Med. 10.1155/2011/238020

Gulcin, I., R. Elias, A. Gepdiremen, L. Boyer and E. Koksal, 2007. A comparative study on the antioxidant activity of fringe tree (Chionanthus virginicus L.) extracts. Afr. J. Biotechnol., 6: 410-418.
Direct Link  |  

Huda-Faujan, N., A. Noriham, A.S. Norrakiah and A.S. Babji, 2009. Antioxidant activity of plants methanolic extracts containing phenolic compounds. Afr. J. Biotechnol., 8: 484-489.
Direct Link  |  

Ismail, A., N.W. Tiong, S.T. Tan and A. Azlan, 2009. Antioxidant properties of selected non-leafy vegetables. J. Nutr. Food Sci., 39: 176-180.
CrossRef  |  

Khalili, R.M.A., A.B. Che Abdullah and A. Abdul Manaf, 2012. Total antioxidant activity, total phenolic content and radical scavenging activity both flesh and peel of red pitaya, white pitaya and papaya. Int. J. Pharmacy Pharm. Sci., 4: 113-122.
Direct Link  |  

Lee, K.H., A.M. Padzil, A. Syahida, N. Abdullah and S.W. Zuhainis et al., 2011. Evaluation of anti-inflammatory, antioxidant and antinociceptive activities of six Malaysian medicinal plants. J. Med. Plants Res., 5: 5555-5563.

Liyana-Pathirana, C. and F. Shahidi, 2005. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chem., 93: 47-56.
CrossRef  |  Direct Link  |  

Sannigrahi, S., U.K. Mazumder, D.K. Pal, S. Parida and S. Jain, 2010. Antioxidant potential of crude extract and different fractions of Enhydra fluctuans Lour. Iran. J. Pharm. Res., 9: 75-82.
Direct Link  |  

Shafekh, S.E., R.M.A. Khalili, C.C.W. Catherine, Z.A. Siti Syakiroh and A.U. Habibah et al., 2012. Total phenolic content and in vitro antioxidant activity of Vigna sinensis. Int. Food Res. J., 19: 1393-1400.
Direct Link  |  

Zin, Z.M., A. Abdul-Hamid and A. Osman, 2002. Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chem., 78: 227-231.
CrossRef  |  Direct Link  |  

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