R. Mohd Adzim Khalili
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
S. Emynur Shafekh
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
A.H. Norhayati
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
I. Mohd Fatahudin
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
R. Rahimah
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
H. Norkamaliah
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
A. Nor Azimah
Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
ABSTRACT
Psophocarpus tetragonolobus also known as the Asparagus pea or winged pea (Family: Fabaceae) most widely grown in hot, humid equatorial countries like Malaysia. It provides a source of vitamin A, vitamin C, calcium and iron. The antioxidant potential of 75% methanol extract, ethyl acetate and chloroform soluble fractions of P. tetragonolobus was screened for in-vitro antioxidant activity using total phenolic content, ferric reducing power, 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay, Ferric Thiocyanate (FTC) and Thiobarbituric Acid (TBA) tests. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (1.69 mg/g GAE in concentration 0.5 mg/mL) higher than methanol and chloroform fractions. Antioxidant concentration was found highest in ethyl acetate extract (27.12 mM) using FRAP method, while chloroform extract has the highest antioxidant level (4.48 mM) using ABTS assay. Ethyl acetate extract of P. tetragonolobus was found to have highest antioxidant activity (7.27%) compared to methanol and chloroform using FTC method. While, TBA results shows no difference in antioxidant activity of all extract of P. tetragonolobus.
PDF References Citation
How to cite this article
R. Mohd Adzim Khalili, S. Emynur Shafekh, A.H. Norhayati, I. Mohd Fatahudin, R. Rahimah, H. Norkamaliah and A. Nor Azimah, 2013. Total Phenolic Content and In vitro Antioxidant Activity of
Winged Bean (Psophocarpus tetragonolobus). Pakistan Journal of Nutrition, 12: 416-422.
DOI: 10.3923/pjn.2013.416.422
URL: https://scialert.net/abstract/?doi=pjn.2013.416.422
DOI: 10.3923/pjn.2013.416.422
URL: https://scialert.net/abstract/?doi=pjn.2013.416.422
REFERENCES
- Ismail, A., N.W. Tiong, S.T. Tan and A. Azlan, 2009. Antioxidant properties of selected non-leafy vegetables. J. Nutr. Food Sci., 39: 176-180.
CrossRef - Balasundram, N., K. Sundram and S. Samman, 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem., 99: 191-203.
CrossRefDirect Link - Shafekh, S.E., R.M.A. Khalili, C.C.W. Catherine, Z.A. Siti Syakiroh and A.U. Habibah et al., 2012. Total phenolic content and in vitro antioxidant activity of Vigna sinensis. Int. Food Res. J., 19: 1393-1400.
Direct Link - Farag, R.S., A.Z.M.A. Badei, F.M. Hewedi and G.S.A. El-Baroty, 1989. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. J. Am. Oil Chem. Soc., 66: 792-799.
CrossRefDirect Link - Gulcin, I., R. Elias, A. Gepdiremen, L. Boyer and E. Koksal, 2007. A comparative study on the antioxidant activity of fringe tree (Chionanthus virginicus L.) extracts. Afr. J. Biotechnol., 6: 410-418.
CrossRefDirect Link - Huda-Faujan, N., A. Noriham, A.S. Norrakiah and A.S. Babji, 2009. Antioxidant activity of plants methanolic extracts containing phenolic compounds. Afr. J. Biotechnol., 8: 484-489.
Direct Link - Liyana-Pathirana, C. and F. Shahidi, 2005. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chem., 93: 47-56.
CrossRefDirect Link - Khalili, R.M.A., A.B. Che Abdullah and A. Abdul Manaf, 2012. Total antioxidant activity, total phenolic content and radical scavenging activity both flesh and peel of red pitaya, white pitaya and papaya. Int. J. Pharmacy Pharm. Sci., 4: 113-122.
Direct Link - Zin, Z.M., A. Abdul-Hamid and A. Osman, 2002. Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chem., 78: 227-231.
CrossRefDirect Link - Sannigrahi, S., U.K. Mazumder, D.K. Pal, S. Parida and S. Jain, 2010. Antioxidant potential of crude extract and different fractions of Enhydra fluctuans Lour. Iran. J. Pharm. Res., 9: 75-82.
Direct Link - Aris, S.R.S., S. Mustafa, N. Ahmat, F.M. Jaafar and R. Ahmad, 2009. Phenolic content and antioxidant activity of fruits of Ficus deltoidea var angustifolia sp. Malaysian J. Anal. Sci., 13: 146-150.
Direct Link - Ganie, S.A., E. Haq, A. Masood, A. Hamid and M.A. Zargar, 2011. Antioxidant and protective effect of ethyl acetate extract of Podophyllum hexandrum rhizome on carbon tetrachloride induced rat liver injury. Evid. Based Complement Altern. Med.
CrossRef