Submit Manuscript

Pakistan Journal of Nutrition

Year: 2013 | Volume: 12 | Issue: 4 | Page No.: 360-364
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Isolation, Characterization and Production of Mannanase from Thermophilic Bacteria to Increase the Feed Quality

Harnentis, Yetti Marlida, Yose Rizal and Maria Endo Mahata

ABSTRACT

Eleven isolates of thermophilic bacteria were isolated from hot spring and collected from West Sumatera of Indonesia. The bacteri isolated were grown on medium agar containing locust bean gum at 60°C for 48 hours to examine their mannanase production by using Congo red test. It was found that four isolates showed positive results with clear zone around the cultures. All isolates were evaluated their mannanase activity by growing in liquid medium supplemented with 0.5% locust bean gum as mannan source. It was found that isolate SM-1.4 displayed that highest enzyme activity of 119,44 U/ml and specific activity of 19.55 U/mg protein. The optimal growth conditions were at 60°C, pH 7 and 24 hours of incubation. According to the morphological studies, the isolate SM-1.4 was primarily identified as the genus Bacillus. The bacteria was gram negative and has endospore.
PDF References Citation

Search

REFERENCES

  1. Abdolvahab, M.H., J. Fooladi, S. Gharavi and M. Sadeghizadeh, 2010. Growth condition optimization of the Iranian Thermophilic Geobacillus sp MKK with the aim of characterizing the DNA polymerase I enzyme and its applications in PCR. World Applied Sci. J., 11: 354-361.
    Direct Link
  2. Ademark, P., A. Varga, J. Medve, V. Harjunpaa, T. Drakenberg, F. Tjerneld and H. Stalbrand, 1998. Softwood hemicellulose-degrading enzymes from Aspergillus niger: Purification and properties of β-mannanase. J. Biotechnol., 63: 199-210.
    PubMed
  3. Araki, K., K.O. Fujiyama and T. Yoshida, 1996. Charactristic of the oligosaccharide structure of jack bean alpha-manosidase with some lectins. J. Ferment Bioeng., 78: 225-258.
  4. Akhmaloka, A. Suharto, S. Nurbaiti, I.N. Tika and F.M. Warganegara, 2006. Ribotyping identification of thermophilic bacterium from papandayan crater. ITB J. Eng. Sci., 38: 1-10.
    Direct Link
  5. Bhat, M.K., 2000. Cellulases and related enzymes in biotechnology. Biotechnol. Adv., 18: 355-383.
    CrossRefPubMedDirect Link
  6. Bicho, P.A., T.A. Clark, K. Mackie, H.W. Morgan and R.M. Daniel, 1991. The characterisation of a thermostable endo-β-1,4-mannanase cloned from Caldocellum saccharolyticum. Applied Microbiol. Biotechnol., 36: 337-343.
    CrossRef
  7. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
    CrossRefPubMedDirect Link
  8. Buckeridge, M.S., H. Pessoa dos Santos and M.A.S. Tine, 2000. Mobilisation of storage cell wall polysaccharides in seeds. Plant Physiol. Biochem., 38: 141-156.
    CrossRef
  9. Capoe, P., M. Kubackova and J. Alfoldi, 2000. Galactomannan from the secondary cell wall of Picea abies L. karst. Carbohydr. Res., 329: 635-645.
  10. Chandra, M.R.S., Y.S. Lee, I.H. Park, Y. Zhou, K.K. Kim and Y.L. Choi, 2011. Isolation, purification and characterization of a thermostable β-mannanase from Paenibacillus sp. DZ3. J. Korean Soc. Applied Biol. Chem., 54: 325-331.
    CrossRef
  11. Chanzy, H., M. Dube, R.H. Marchessault and J.F. Revol, 2004. Single crystals and oriented crystallization of ivory nut mannan. Biopolymers, 18: 887-898.
    CrossRef
  12. Dingle, J.G., 1995. The Use of Enzymes for Better Performance of Poultry. In: Queensland Poultry Science Symposium, Dingle, J.G. (Ed.). The University of Queensland, Queensland, Australia, pp: 1-14.
  13. Duffaud, G.D., C.M. McCutchen, P. Leduc, K.N. Parker and R.M. Kelly, 1997. Purification and characterization of extremely thermostable β-mannanase and α-galactosidase from the hyperthermophilic Eubacterium Thermatoga neopolitana 5068. Applied Environ. Microbiol., 63: 169-177.
    PubMed
  14. Eeckhout, M., M. DeSchrijver and E. Vanderbeke, 1995. The influence of process parameters on stability of feed enzymes during steam pelleting. Proceedings of the 2nd European Symposium on Feed Enzymes, October 25-27, 1995, Noordwijkerhout, Netherlands, pp: 163-169.
  15. Gubitz, G.M., M. Hayn, G. Urbanz and W. Steiner, 1996. Purification and properties of an acidic β-mannanase from Sclerotium rolfsii. J. Biotechnol., 45: 165-172.
    Direct Link
  16. Jiang, Z.Q., Y. Wei, D. Li, L. Li, P. Chai and I. Kusakake, 2006. High-level production, purification and characterization of a thermostable β-mannanase from the newly isolated Bacillus subtilis WY34. Carbohydr. Polym., 66: 88-96.
    CrossRefDirect Link
  17. Khanongnuch, C., K. Asada, H. Tsuruga, T. Ooi, S. Kinoshita and S. Lumyong, 1998. β-mannanase and xylanase of Bacillus subtulis 5H active for bleaching of crude pulp. J. Ferment. Boeing., 5: 461-466.
  18. Kumar, A., J.G. Dingle, K. Wiryawan and D.C. Creswell, 1997. Enzymes for improved nutritional value of layer diets. Proceedings of the Queensland Poultry Science Symposium, Volume 6, (QPSS'97), University of Queensland, Queensland, Australia.
  19. Kurakake, M. and T. Komaki, 2001. Production of beta-mannanase and beta-mannosidase from Aspergillus awamori K4 and their properties. Curr. Microbiol., 42: 377-380.
    PubMed
  20. McCleary, B.V., 1988. Synthesis of β-D-mannopyranosidases for the assay of β-D-mannosidase and exo-β-D-mannanase. Methods Enzymol., 160: 515-518.
  21. Meenakshi, G. Singh, A. Bhalla and G.S. Hoondal, 2010. Solid state fermentation and characterization of partially purified thermostable mannanase from Bacillus sp. MG-33. Bioresources, 5: 1689-1701.
    Direct Link
  22. Mendoza, N.S., M. Arai, T. Kawaguchi, F.S. Cubol, E.G. Panerio, T. Yoshida and L.M. Joson, 1994. Isolation of mannan-utilizing bacteria and the culture conditions for mannanase production. World J. Microbiol. Biotechnol., 10: 51-54.
    CrossRef
  23. Phothichitto, K., S. Nitisinprasert and S. Keawsompong, 2006. Isolation, screening and identification of mannanase producing microorganisms. Kasetsart J. Nat. Sci., 40: 26-38.
    Direct Link
  24. Smogyi, M., 1952. Notes on sugar determination. J. Biol. Chem., 195: 19-23.
    PubMedDirect Link
  25. Sumardi, A.S., T.S. Maggy and P. Tresnawati, 2006. Purification and characterization of the extracellular beta-mannanase from a thermophilic bacterium, Geobacillus stearothermophilus L-07. J. Microbiol. Indonesia, 11: 57-62.
  26. Tamaru, Y., T. Araki, H. Amagoi, H. Mori and T. Morishita, 1995. Purification and characterization of an extracellular β-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138. Applied Environ. Microbiol., 61: 4454-4458.
    Direct Link

Search

Related Articles

Leave a Comment

Your email address will not be published. Required fields are marked *