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Research Article

Production of Lactic Acid by a Local Isolate of Lactobacillus plantarum Using Cheap Starchy Material Hydrolysates

Amal Kadhim G. Al-Asady
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Some cheap starchy materials like sorghum grains and wheat bran were degraded by crude glucoamylase of a local isolate of Mucor sp., then Lactic Acid (LA) was produced by a local isolate of L. plantarum using sorghum flour hydrolysate, sorghum starch hydrolysate, soluble starch hydrolysate, wheat bran hydrolysate and date syrup with 10% reducing sugars. The yield of LA increased to 37.2 g/100 reducing sugars by using sorghum flour hydrolysate as a basal medium supplemented with (0.6+0.6)% yeast extract+(NH4)2HPO4 and 0.06% MgSO4.7H2O, reducing sugars was 5%. The fermentation temperature was 30°C/96 h. Results indicated that using hydrolysates mixtures of sorghum flour and wheat bran improved LA fermentation. The yield of LA was 92.5 g by using sorghum flour and 50% wheat bran. Paper chromatography indicated that LA was the unique organic acid in the fermented broth.

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  How to cite this article:

Amal Kadhim G. Al-Asady , 2012. Production of Lactic Acid by a Local Isolate of Lactobacillus plantarum Using Cheap Starchy Material Hydrolysates. Pakistan Journal of Nutrition, 11: 88-93.

DOI: 10.3923/pjn.2012.88.93



1:  Adsul, M.G., A.J. Varma and D.V. Gukhale, 2007. Lactic acid production from waste sugarcane bagasse derived cellulose. Green Chem., 9: 58-62.
CrossRef  |  Direct Link  |  

2:  Adthalungrong, C. and S. Temviriyanukul, 2010. Optimization of lactic acid production from tapioca starch hydrolysate by Lactobacillus casei TISTR 453. KKU. Res. J., 15: 436-445.
Direct Link  |  

3:  Aksu, Z. and T. Kutsal, 1986. Lactic acid production from molasses utilizing L. delbrueckii. Biotechnol. Lett., 8: 157-160.

4:  Altaf, M., B.J. Naveena, M. Venkateshwar, E.V. Kumar and G. Reddy, 2006. Single step fermentation of starch to L (+) lactic acid by Lactobacillus amylophilus GV6 in SSF using inexpensive nitrogen sources to replace peptone and yeast extract-optimization by RSM. Proc. Biochem., 41: 465-472.
Direct Link  |  

5:  Amrane, A. and Y.A. Prigent, 1996. A novel concept of bioreactor: Specialized function two-stage continuous reactor and its application to lactose conversion in to lactic acid. J. Biotechnol., 45: 195-203.
Direct Link  |  

6:  Dumbrepatil, A., M. Adsul, S. Chaudhari, J. Khire and D. Gokhale, 2008. Utilization of molasses sugar for lactic acid production by Lactobacillus delbrueckii subsp. Delbrueckii mutant Uc-3 in batch fermentation. Applied Environ. Microbiol., 74: 333-335.
CrossRef  |  Direct Link  |  

7:  Fitzpatrick, J.J., M. Ahrens and S. Smith, 2001. Effect of manganese on Lactobacillus casei fermentation to produce lactic acid from whey permeate. Proc. Biochem., 36: 671-675.
CrossRef  |  Direct Link  |  

8:  Hofvendahl, K. and B. Hahn-Hagerdal, 2000. Factors affecting the fermentative lactic acid production from renewable resources. Enzymes Microb. Technol., 26: 87-107.
CrossRef  |  PubMed  |  Direct Link  |  

9:  John, R.P., K.M. Nampoothiri, A.S. Nair and A. Pandey, 2005. L (+) lactic acid production using Lactobacillus casei in solid state fermentation. Biotechnol. Lett., 27: 1685-1688.
PubMed  |  

10:  Kleerebezem, M., J. Boekhorst, R. Kranenburg, D. Molenaar and O.P. Kuipers et al., 2003. Complete genome sequence of Lactobacillus plantarum. WCFS1: Proc. Natl. Acad. Sci. USA., 100: 1990-1995.
PubMed  |  Direct Link  |  

11:  Kwon, S., I.K. Yoo, W.G. Lee, H.N. Chang and Y.K. Chang, 2001. High rate continuous production of lactic acid by Lactobacillus bulgaricusin two-stage membrane cell-cycle bioreactor. Biotechnol. Bioeng., 73: 25-34.

12:  Montet, D., G. Loiseau and N. Kakhia-Rozis, 2006. Microbial Technology of Fermented Vegetables. In: Microbial Biotechnology in Horticulture, Ray, R.C. and O.P. Ward (Eds.). Science Publishers, Inc., New Hampshire, USA, pp: 309-343

13:  Narayanan, N., P.K. Roychoudhury and A. Srivastava, 2004. L (+) Lactic acid fermentation and its product polymerization. Electron. J. Biotechnol., 7: 167-179.
CrossRef  |  Direct Link  |  

14:  Ohkouchi, Y. and Y. Inoue, 2006. Direct production of L (+) lactic acid from starch and food wastes using Lactobacillus manivotivorans LMG18011. Bioresour. Technol., 97: 1554-1562.
Direct Link  |  

15:  Panda, S.H. and R.C. Ray, 2008. Direct conversion of raw starch to lactic acid by Lactobacillus plantarum MTCC 1407 in semi-solid fermentation using sweet potato (Ipomoea batatas L.) flour. J. Sci. Industrial Res., 67: 531-537.

16:  Qi, B.K., R. Yao, M. Lai and S. Deng, 2009. Effect of tween 80 on lactic acid by Lactobacillus casei. Songklanakarin J. Sci. Technol., 42: 85-89.

17:  Rogosa, M., 1974. Lactobacillus. In: Bergys Manual of determinative Bacteriology, Buchanan, R.E. and N.E. Gibbons (Eds.). The Williams and Wlkins: Co., Baltimore, pp: 576-593

18:  Shamala, T.R. and K.R. Sreekantiah, 1987. Degradation of starchy substrates by a crude enzyme preparation and utilization of the hydrolysates for lactic acid fermentation. Enzyme Microb. Technol., 9: 726-729.
Direct Link  |  

19:  Shamala, T.R. and K.R. Sreekantiah, 1988. Fermentation of starch hydrolysates by Lactobacillus plantarum. J. Ind. Microbiol., 3: 175-178.
CrossRef  |  Direct Link  |  

20:  Venkatesh, K.V., 1997. Simultaneous saccharification and fermentation of cellulose to lactic acid. Bioresour. Technol., 62: 91-98.
CrossRef  |  Direct Link  |  

21:  Wee, Y.J., J.N. Kim and H.W. Ryu, 2006. Biotechnological production of lactic acid and its recent applications. Food Technol. Biotechnol., 44: 163-172.
Direct Link  |  

22:  Yang, P. and P. Seib, 1995. Low-input wet milling of grain sorghum for readily accessible starch and animal feed. Cereal Chem., 72: 498-503.
Direct Link  |  

23:  Zhou, S.K., K.T. Shanmugan, L.P. Yomano, T.B. Grabar and L.O. Ingram, 2006. Fermentation of 12% (w/v) glucose to 1.2M lactate by Escherichia coli strain SZ194 using mineral salts medium. Biotechnol. Lett., 28: 663-670.
PubMed  |  

24:  Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31: 426-428.
CrossRef  |  Direct Link  |  

25:  John, R.P., K.M. Nampoothiri and A. Pandey, 2006. Solid-state fermentation for l-lactic acid production from agro wastes using Lactobacillus delbrueckii. Process Biochem., 41: 759-763.
CrossRef  |  

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