Beitullah Alipour
Department of Nutrition, Faculty of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
Aida Ghaffari
Department of Nutrition, Faculty of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
Alireza Ostadrahimi
Department of Nutrition, Faculty of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
Abdolrasoul Safaiyan
Department of Vital Statistics and Epidemiology, Faculty of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
Jabiz Modaresi
Department of Nutrition, Faculty of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
Elnaz Vaghef Mehrabany
Department of Nutrition, Faculty of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
ABSTRACT
Gastric cancer is the fourth most common malignancy and the second leading cause of mortality among cancer patients in the world. Nutritional and epidemiological studies have indicated that zinc, iron and copper status modulates the risk of developing cancer and on the other hand, apoptosis has been reported to play a decisive role in precancerous changes. The aim of this cross-sectional study was to investigate whether there is any relationship between serum zinc, iron and copper levels and apoptosis (as an early indicator of gastric cancer changes) in human gastric mucosa. This cross-sectional study was conducted on 62 subjects with over 18 years of age, referred to 2 hospitals (Shahid Madani and Imam Reza hospitals) in Tabriz, Iran between October and December 2008 to undergo an upper gastrointestinal endoscopy. Serum levels of zinc, iron and copper were measured by atomic absorption spectroscopy and apoptosis was detected by TUNEL technique. Stepwise regression was exploited to access the relationship between apoptosis rate and serum zinc, iron and copper levels. Mean number of apoptotic (TUNEL positive) cells, serum zinc, iron and copper levels were 2.15±0.22, 111.47±2.18 (μg/dl), 145.20±5.24 (μg/dl) and 137.52±3.04 (μg/dl) respectively. The present study found no relationship between the rate of apoptosis and baseline serum levels of neither of zinc, iron and copper. Our study showed that serum zinc, iron and copper levels didnt affect apoptosis in human gastric mucosa. It is suggested that more interventional and controlled studies be done.
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How to cite this article
Beitullah Alipour, Aida Ghaffari, Alireza Ostadrahimi, Abdolrasoul Safaiyan, Jabiz Modaresi and Elnaz Vaghef Mehrabany, 2011. Relationship Between Serum Zinc, Iron and Copper Level and Apoptosis in Human Gastric Mucosa: A Cross-Sectional Study. Pakistan Journal of Nutrition, 10: 919-924.
DOI: 10.3923/pjn.2011.919.924
URL: https://scialert.net/abstract/?doi=pjn.2011.919.924
DOI: 10.3923/pjn.2011.919.924
URL: https://scialert.net/abstract/?doi=pjn.2011.919.924
REFERENCES
- Akiba, S., K. Neriishi, W.T. Blot, M. Kabuto, R.G. Stevens, H. Kato and E.C. Land, 1991. Serum ferritin and stomach cancer risk among a Japanese population. Cancer, 67: 1707-1712.
PubMed - Bir, F., N. Calli-Demirkan, A.C. Tufan, M. Akbulut and N.L. Satiroglu-Tufan, 2007. Apoptotic cell death and its relationship to gastric carcinogenesis. World J. Gastroenterol, 13: 3183-3188.
PubMed - Brenner, H., D. Rothenbacher and V. Arndt, 2009. Epidemiology of stomach cancer. Methods Mol. Biol., 472: 467-477.
CrossRefDirect Link - Clogg, M.S., C.L. Keen and I.S. Hurley, 1989. Biochemical pathologies of zinc deficiencies. In: Zinc in Human Biology, In: Mills, CF. (Eds.), International Life Science Institute, London.
Direct Link - Duchateau, J., G. Delespesse, R. Vrijens and H. Collet, 1981. Beneficial effects of oral zinc supplementation on the immune response of old people. Am. J. Med., 70: 1001-1004.
Direct Link - Haines, A.P., S.G. Thompson, T.K. Basu and R. Hunt, 1982. Cancer, retinal binding protein, zinc and copper. Lancet, 1: 52-53.
CrossRef - Halsted, J.A. and J.C. Smith, 1970. Plasma-zinc in health and disease. Lancet, 295: 322-324.
CrossRef - Hann, H.W.L., C.Y. Kim, W.T. London and B.S. Blumberg, 1989. Increased serum ferritin in chronic liver disease: A risk factor for primary hepatocellular carcinoma. Int. J. Cancer, 43: 376-379.
CrossRef - Herbay, A. and J. Rudi, 2000. Role of apoptosis in gastric epithelial turnover. Microsc. Res. Tech., 48: 303-311.
PubMed - Herrinton, L.J., G.D. Friedman, D. Baer and J.V. Selby, 1995. Transferrin saturation and risk of cancer. Am. J. Epidemiol., 142: 692-698.
Direct Link - Huang, Y.L., J.Y. Sheu and T.H. Lin, 1999. Association between oxidative stress and changes of trace elements in patients with breast cancer. Clin. Biochem., 32: 131-136.
CrossRefPubMedDirect Link - Jayadeep, A., P.K. Raveendran, S. Kannan, K.R. Nalinakumari, B. Mathew, N.M. Krishnan and V.P. Menum, 1997. Serum levels of copper, zinc, iron and ceruplasmin in oral leukoplakia and squamous cell carcinoma. J. Exp. Clin. Cancer Res., 16: 295-300.
Direct Link - Johnson, M.A., J.G. Fischer and S.E. Kays, 1992. Is copper an antioxidant nutrient? Crit. Rev. Food Sci. Nutr., 32: 1-31.
CrossRef - Knekt, P., A. Reunanen, H. Takkunen, A. Aroma, M. Heliovara and T. Hakulinen, 1994. Body iron stores and risk of cancer. Int. J. Cancer, 56: 379-382.
PubMed - Kok, F.J., C.M.V. Duijn, A. Hofman, G.B. Van Det Voet, F.A. Wolff, C.H. Paays and H.A. Valkenburg, 1988. Serum copper and zinc and the risk of death from cancer and cardiovascular disease. Am. J. Epidemiol., 128: 352-359.
Direct Link - Linder, M.C. and M. Hazegh-Azam, 1996. Copper biochemistry and molecular biology. Am. J. Clin. Nutr., 63: 7975-8115.
Direct Link - Liu, H., D.S. Merrell, C. Semino-Mora, M. Goldman, A. Rahman, S. Mogs and A. Dubois, 2009. Diet synergistically affects Helicobacter pylori-induced gastric carcinogenesis in nonhuman primates. Gastroenterology, 137: 1367-1379.
CrossRef - Magalora, T., V. Bella, A. Brtkova, I. Beno, M. Kudlackova and K. Volkovova, 1999. Copper, zinc, superoxide dismutase in precancerous, benign disease and gastric, colorectal and breast cancer. Neoplasma, 46: 100-104.
Direct Link - Malekzadeh, R., M.H. Derakhshan and Z. Malekzadeh, 2009. Gastric cancer in Iran: Epidemiology and risk factors. Arch. Iran. Med., 12: 576-583.
PubMedDirect Link - Margalioth, E.J., J.G. Schenker and M. Chevion, 1983. Copper and zinc levels in normal and malignant tissues. Cancer, 52: 868-872.
CrossRefPubMedDirect Link - Merk, K., B. Mattsson, A. Mattsson, G. Hold, B. Gullbring and M. Bjorkholm, 1990. The incidence of cancer among blood donors. Int. J. Epidemiol., 19: 505-509.
PubMed - Nardone, G. and D. Compare, 2008. Epigenetic alterations due to diet and Helicobacter pylori infection in gastric carcinogenesis. Expert Rev. Gastroenterol. Hepatol., 2: 243-248.
PubMed - Nelson, R.L., F.G. Davis, E. Sutter, L.H. Sobin, J.W. Kikenhall and P. Bowen, 1994. Body iron stores and risk of colonic neoplasia. J. Nat. Cancer Insti., 86: 455-460.
PubMed - Pelucchi, C., I. Tramacere, P. Bertuccio, A. Tavani, E. Negri and C.L. Vecchia, 2009. Dietary intake of selected micronutrients and gastric cancer risk: An Italian case-control study. Ann. Oncol., 20: 160-165.
CrossRef - Prasad, A.S., 1983. Clinical, biochemical and nutritional spectrum of zinc deficiency in human subjects: An update. Nutr. Rev., 41: 197-208.
CrossRef - Schrauzer, G.N., D.A. White and C.J. Schneider, 1977. Cancer mortality correlation studies. IV: Associations with dietary intakes and blood levels of certain trace elements, notably se-antagonists. Bioinorg. Chem., 7: 35-56.
Direct Link - Selby, J.V. and G.D. Friedman, 1988. Epidemiologic evidence of an association between body iron stores and risk of cancer. Int. J. Cancer, 41: 677-682.
PubMed - Stevens, R.G., B.I. Graubard, M.S. Micozzi, K. Neriishi and B.S. Blumberg, 1994. Moderate elevation of body iron level and increased risk of cancer occurrence and death. Int. J. Cancer, 56: 364-369.
CrossRefDirect Link - Stevens, R.G. and D.R. Kalkwarf, 1990. Iron, radiation and cancer. Environ. Health Persp., 87: 291-300.
Direct Link - Sunderman, F.W. Jr., 1995. The influence of zinc on apoptosis. Ann. Clin. Lab. Sci., 25: 134-142.
Direct Link - Swauger, J.E., P.M. Dolan, J.L. Zweier, P. Kuppusamy and T.W. Kensler, 1991. Role of the benzoyloxyl radical in DNA damage mediated by benzoyl peroxide. Chem. Res. Toxicol., 4: 223-228.
CrossRef - Toyokuni, S., 1996. Iron-induced carcinogenesis: The role of redox regulation. Free Radic. Biol. Med., 20: 553-566.
Direct Link - Truong-Tran, A.Q., L.H. Ho, F. Chai and P.D. Zalewski, 2000. Cellular zinc fluxes and the regulation of apoptosis/gene-directed cell death. J. Nutr., 130: 1459-1466.
Direct Link - Xu, A.G., S.G. Li, J.H. Liu and A.H. Gan, 2001. Function of apoptosis and expression of the proteins Bcl-2, P53 and C-myc in the development of gastric cancer. World J. Gastroenterol., 7: 403-406.
PubMed