Subscribe Now Subscribe Today
Research Article

Analysis of Protein from Pea (Pisum sativum) and Gram (Cicer arietinum) by Electrophoresis and Paper Chromatography

Afsheen Mushtaque Shah, Muhammad Saleh Memon, Allah Nawaz Memon, Abdul Wahab Ansari, Basir Ahmed Arain, Shaista Khan and Ibtassam Tahir
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Protein is not only essential compound of animal but it is also important constitute of plants. Plants are good source of proteins. These proteins are mostly analyzed and identified from the fruits, seeds or any other parts of plants which are mostly used in diet but there are many other types of protein which are synthesized by other parts also, if one can know about synthesis of those proteins at different growth periods of different parts of plant then one may get interesting results. In this study stem of two plants were used for the analysis of protein i.e Pea (Pisum sativum) and Gram (Cicer arietinum). Molecular weight of protein was analyzed by SDS/Polyacrylamide Gel Electrophoresis and protein was stained with Lawsone dye by paper chromatography. Lawsone dye is protein dye which is mostly used for the staining of hair, skin and nails protein but in this research same dye was used for the staining of plant protein through paper chromatography.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Afsheen Mushtaque Shah, Muhammad Saleh Memon, Allah Nawaz Memon, Abdul Wahab Ansari, Basir Ahmed Arain, Shaista Khan and Ibtassam Tahir, 2011. Analysis of Protein from Pea (Pisum sativum) and Gram (Cicer arietinum) by Electrophoresis and Paper Chromatography. Pakistan Journal of Nutrition, 10: 860-863.

DOI: 10.3923/pjn.2011.860.863


1:  Ali, S.I., 1977. Flora of West Pakistan, Papilionaceae. Vol. 100, Stewart Herbarium, Karachi, pp: 260-264.

2:  Atta, S., S. Maltoese, P. Marget and R. Cousin, 2004. 15NO3 assimilation by the field pea Pisum sativum L. Agronomie, 24: 85-92.
CrossRef  |  

3:  Brancaccio, R.R., L.H. Brown, Y.T. Chang, J.P. Fogelman, E.A. Mafong and D.E. Cohen, 2002. Identification and quantification of para-phenylenediamin in a temporary black henna tattoo. Am. J. Contact Dermat., 13: 15-18.
Direct Link  |  

4:  Catherine, C.J., 2005. The Henna Page, How to Mix Henna. Henna Publication, Ohio, USA.

5:  Groth, D.S.F., R.G. Wbster and A. Datyner, 1963. Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. J. Biochem. Biophys Acta., 14: 377-391.
PubMed  |  

6:  Dhumal, N.R., A.V. Todkary, S.Y. Rane and S.P. Gjji, 2005. Hydrogen bonding motif in 2-hydroxy-1,4 naphthoquinone. J. Theor. Chem A C., 113: 161-166.
CrossRef  |  

7:  Hazra, A., 2002. Adverse reaction to Henna. J. Pharmco., 34: 436-437.
Direct Link  |  

8:  Jain, J.L., J. Sunjay and J. Nitin, 2006. Fundamental of Biochemistry. S. Chand & Co. Ltd., India, pp: 111-229.

9:  Kang, J.I. and M.H. Lee, 2006. Quantification of para-phenylenediamine and heavy metals in Henna dye. J. Contact Dermat., 55: 26-29.
PubMed  |  

10:  Muilerman, H.G., H.G.T. Hart and W.V. Dijk, 1982. Specific detection of inactive enzyme protein after polyacrylamide gel electrophoresis by a new enzyme-immunoassay method using unspecific antiserum and partially purified active enzyme: Application to rat liver phosphodiesterase. Anal. Biochem., 120: 46-51.
CrossRef  |  

11:  Nikkel, A.F., F. Henry and G.E. Pierard, 2001. Allergic reactions to decorative skin painting. J. Europ. Acad. Dermat. Venereo, 15: 140-142.
PubMed  |  

12:  Osman, A.M. and P.C.V. Noort, 2003. Evidence in redox cycling of Lawsone (2-Hydroxy-1,4-naphthoqinone) in the presence of the hypoxanthine/xanthine oxidase system. J. Appl. Toxic. Pub. Med., 23: 209-212.

13:  Osset, M., M. Pinol, M.J.M. Falllon, R. de Llorens and C.M. Cuchillo, 1989. Inference of the carbohydrate moiety in coomassie brilliant blue R-250 protein staining. J. Electrophoresis, 10: 271-273.

14:  Pandey, A., M.K. Choudhary, D. Bushan, A. Chattopadhyay, S. Chakraborty, A. Datta and N. Chakraborty, 2006. The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins. J. Proteome Res., 5: 3301-3311.
PubMed  |  

15:  Petkewich, R., 2006. Henna dye derived from green leaves is used to decorate the body with intricate designs. J. Am. Chem. Soc., 84: 15-20.

16:  Righetti, P.G. and J.W. Drysdale, 1974. Isoelectric focusing in gels. J. Chromatogr., 98: 271-321.

17:  Rund, D., T. Schaap, N. Da'as, D.B. Yehuda and J. Kalish, 2007. Plasma exchange as treatment of Lawsone (Henna) intoxication. J. Clin. Apheresis, 22: 243-245.
PubMed  |  

18:  Sawhney, S.K. and R. Singh, 2005. Introductory Practical Biochemistry. 2nd Edn., Narosa Publishing House Pvt. Ltd., New Delhi, India, ISBN-13: 9781842652459, Pages: 452.

19:  Schiltz, S., N. Munier-Jolain, C. Jeudy, J. Burstin and C. Salon, 2005. Dynamics of exogenous nitrogen partitioning and nitrogen remobilization from vegetative organs in pea revealed by 15N in vivo labeling throughout seed filling. J. Plant Physio., 137: 1463-1473.
CrossRef  |  

20:  Stayanarayana, U. and U. Chakrapati, 2007. Biochemistry. 3rd Edn., Books and Allied. Ltd.,. pp: 42-43..

21:  Syed, R.B., 1989. Medicinal plant. Medical and Poisonous Plant of Pakistan. 1st (Edn.).,. Printes Karachi, Pakistan, pages: 259.

22:  Vasudevan, D.M. and S. SreeKumari, 2005. T/B of Biochemistry. 4th (Edn.)., Japee Brothers medical Pub, New Delhi, India, pp: 23-48.

23:  Wallis, T.E., 2005. Henna and raspberry leaves. T/B Pharmacognosy. 5th Edn., CBS, India, pp: 144-145.

24:  Willaim, C.E., 2002. Phenol and Phenolic Glycoside: Trease and Evans Pharmacognosy. 15th Edn., WB Saunders, UK, pp: 214-252.

25:  Zor, T. and Z. Selinger, 1996. Linearization of the Bradford protein assay increases its sensitivity: Theoretical and experimental studies. Anal. Biochem., 236: 302-308.
CrossRef  |  Direct Link  |  

©  2020 Science Alert. All Rights Reserved