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Research Article

Multiresidue Analysis of Pesticides by Gas Chromatography with Electron-capture in Rice Samples from Different Geographical Regions of Pakistan

Alia B. Munshi, Hina A. Siddiqi, Fayyaz A. Ansari, Razia Begum and Tanzil H. Usmani
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Since last few years Pakistani rice exporters have become very conscious about the residual level of pesticides in rice and they prefer to have a certificate for residues free rice prior to shipment. 1000 samples of rice were collected from different containers ready to export representing different geographical regions of Pakistan and were tested at the Pakistan Council of Scientific and Industrial Research. The residue levels of 37 chlorinated hydrocarbons including major groups like hexachlorocyclohexane (∑HCH) isomers (α-HCH, β-HCH, γ-HCH and δ-HCH), Dichloro-diphenyl-trichloroethane (∑DDT) congeners (p,p-DDE, o,p-DDT, p,p-DDD and p,p-DDT), heptachlor isomers and Chlordane isomers, aldrin, dieldrin and endrin and chloripyrifos in rice were determined by GC-ECD. Residues of ∑DDT and ∑HCH were detected in 30 and 45% of the samples and during 2005 their average values were found to be 0.001 mg/kg and 0.005 mg/kg repectively. In 2006 level of ∑DDT and ∑HCH was 0.071 mg/kg and 0.191 mg/kg showing increased contamination of these pesticides. Similarly residual level of ∑heptachlor and ∑Chloradane was 0.003 and 0.002 mg/kg in 2005 and these were at the level of 0.191 and -0.005 mg/kg during 2006 repectively. The maximum permissible limit for DDT and HCH metabolites were determined on the basis of an average factor to be 0.2-0.5 mg/kg which is just near to codex limit. Mean recoveries from three replicates ranged from 85.5-106.0%, with coefficients of variation from 0.3-9.9%. The limit of detection was in the range of 0.02-0.01 mg/kg for all organchlorines in average of 37 compounds.

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  How to cite this article:

Alia B. Munshi, Hina A. Siddiqi, Fayyaz A. Ansari, Razia Begum and Tanzil H. Usmani, 2011. Multiresidue Analysis of Pesticides by Gas Chromatography with Electron-capture in Rice Samples from Different Geographical Regions of Pakistan. Pakistan Journal of Nutrition, 10: 851-859.

DOI: 10.3923/pjn.2011.851.859


1:  Battu, B.S., Kang and B.S. Joia, 2005. Chlorine and phosphorus containing pesticides in cereals and legumes imported into Italy. Riv. Soc. Ital Sci. Aliment, 16: 465-470.

2:  Caldas, E.D. and L.C.K.R. Souza, 2004. Chronic dietary risk for pesticide residues in food in Brazil: An update. Food Additives Contaminants, 21: 1057-1064.
CrossRef  |  

3:  Economic Survey of Pakistan, 2007-08. Vegetables retailed in agricultural product markets of Economic Affairs Advisors Wing, Finance Division, Nanjing, China. Bulletin of Environ. Contamination Government of Pakistan, Islamabad.

4:  Darko, G. and O. Akoto, 2008. Dietary intake of organophosphorus pesticide residues through vegetables from Kumasi, Ghana. Food Chem. Toxicol., 46: 3703-3706.
CrossRef  |  Direct Link  |  

5:  Griffitt, K.R., 1983. Miniaturized florisil column cleanup of chlorinated and organophosphate eluates in total diet samples. LIB 2722, FDA, Rockville, MD.

6:  Hussain, M.M., 1992. Studies of the stability of pesticides, their chemical degradation and their residual toxicity under storage conditions of Pakistan. Ph.D. Thesis, University of Karachi, 1992.

7:  Jabbar, A., S.Z. Masud, P. Zahida and M. Ali, 1993. Pesticide residues in cropland soils and shallow groundwater in Punjab Pakistan. Bull. Environ. Contam. Toxicol., 51: 268-273.
PubMed  |  

8:  Jiawei, C., C. Liu, Z. Yang and J. Wang, 2008. Residues and characteristics of organochlorine pesticides in the surface water in the suburb of Beijing. Earth Sci. Frontiers, 15: 242-247.
CrossRef  |  

9:  Kalantzi, O.I., R.E. Alcock, P.A. Johnston, D. Santillo and R.L. Stringer et al., 2001. The global distribution of PCBs and organochlorine pesticides in butter. Environ. Sci. Technol., 35: 1013-1018.
CrossRef  |  

10:  Kathpal, T.S. and B. Kumari, 2009. Monitoring of pesticide residues in vegetarian diet. Environ. Monit. Assess., 151: 19-26.
CrossRef  |  

11:  Munshi, A.B., K. Jamil and R. Zuberi, 2001. Pesticides residues in a variety of food stuff from a Karachi City. J. Chem. Soc. Pak., 23: 95-96.

12:  PCCRARM (Presidential/Congressional Commission on Risk Assessment and Risk Management), 1997. Framework for Environmental Health Risk Management: Final Report. Washington, DC: The Commission.

13:  Phuong, P.K., C.P.N. Son, J.J. Sauvain and J. Tarradellas, 1998. Contamination by PCB's, DDT's and heavy metals in sediments of Ho Chi Minh City's Canals, Viet Nam. Bull. Environ. Contam. Toxicol., 60: 347-354.
CrossRef  |  Direct Link  |  

14:  Ramesh, A., S. Tanabe, H. Murase, A.N. Subramanian and R. Tatsukawa, 1991. Distribution and behaviour of persistent organochlorine insecticides in paddy soil and sediments in the tropical environment: A case study in South India. Environ. Pollut., 74: 293-307.
CrossRef  |  

15:  Rashid, B.M., 2006. Country report on international code of conduct on the distribution and use of pesticides by adviser and director general department of plant protection. Ministry of Food, Agriculture and Livestock, Government of Pakistan.

16:  Shukla, Y., A. Yadav and A. Arora, 2002. Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin. Cancer Lett., 182: 33-41.
CrossRef  |  Direct Link  |  

17:  Wu, W.Z., Y. Xu, K.W. Schramm and A. Kettrup, 1997. Study of sorption, biodegradation and isomerization of HCH in stimulated sediment/water system. Chemosphere, 35: 1887-1894.
CrossRef  |  

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