Subscribe Now Subscribe Today
Research Article

Electrophoretic Analysis of Seed Proteins from Different Varieties of Rice Cultivated in Sindh

Ibtessam Tahir Ansari, Mumtaz Ali Sahito, A.B. Ghanghro, A.N. Memon and Shaista Khan
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

SDS-PAGE electrophoresis is the most effective as well as one of the simplest methods for separating proteins. The extraction and fractionation technique was applied to characterize rice seed proteins. Identification and screening of rice proteins carried out from 14 varieties of rice collected from Nuclear Institute of Agriculture (NIA) Tandojam. Rice seed proteins were extracted with phosphate buffer for total proteins. After quantification, proteins were separated by SDS-PAGE Electrophoresis. Analysis of rice seed proteins was performed on the results of SDS-PAGE using gel documentation system (Biorad) to find out the molecular weight of different varieties of rice followed by cluster analysis using SPSS to find out the diversity among different rice varieties.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Ibtessam Tahir Ansari, Mumtaz Ali Sahito, A.B. Ghanghro, A.N. Memon and Shaista Khan, 2011. Electrophoretic Analysis of Seed Proteins from Different Varieties of Rice Cultivated in Sindh. Pakistan Journal of Nutrition, 10: 663-666.

DOI: 10.3923/pjn.2011.663.666


Athwal, D.S., 1975. Present and future research on rice. Cereal Foods World, 20: 354-361.
Direct Link  |  

Bushuk, W. and R.R. Zillman, 1978. Wheat cultiver identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can. J. Plant Sci., 58: 505-515.

Furukawa, S., T. Mizuma, Y. Kiyokawa, T. Masumura, K. Tanaka and Y. Wakai, 2003. Distribution of storage proteins in low-glutelin rice seed determined using a fluorescent antibody. J. Biosci. Bioeng., 96: 467-473.
PubMed  |  

Houston, D.F. and A. Mohammad, 1970. Purification and partial characterization of a major globulin from rice endosperm. Cereal Chem., 47: 5-11.
Direct Link  |  

Julialo, B.O., E.L. Albano and G.B. Cagampang, 1964. Variability in protein content, amylose content and alkali digestibility of rice varieties in Asia. Philipp. Agric., 48: 234-241.

Juliano B.O., C.C. Ignacio, V.M. Panganiban and C.M. Perez, 1968. Screening for high protein varieties. Cereal Sci. Today, 13: 299-301.

Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
CrossRef  |  Direct Link  |  

Nei, M. and W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA., 76: 5269-5273.
PubMed  |  Direct Link  |  

Oppong-Konadu, E.Y.R., I.K. Akromah, Adu-Dapaah and E. Okai, 2005. Genetic diversity within ghanaian cowpea germplasm based on SDS-PAGE of seed proteins. Afr. Crop Sci. J., 13: 117-123.

Pollard, N.J., C.W. Wrigley, F. Bekes, A. Aumatell and F. MacRitchie, 1996. Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis. Electrophoresis, 17: 221-223.
PubMed  |  

Qu, L.Q., H. Satoh, M. Ogawa and X.L. Wei, 2001. Improved electrophoretical analysis for rice seed storage glutelin. Acta Genet. Sin., 28: 730-737.
PubMed  |  

Romesburg, H.C., 1990. Cluster Analysis for Researchers. Robert E. Krieger Publishing Co., Florida, USA.

Steenson, D.F. and S.K. Sathe, 1995. Characterization and digestibility of basmati rice (Oryza sativa L. var. Dehraduni) storage proteins. Cereal Chem., 72: 275-280.
Direct Link  |  

Wrigley, C.W., J.C. Autran and W. Bushuk, 1982. Identification of cereal varieties by gel electrophoresis of the grain proteins. Adv. Cereal Sci. Technol., 5: 211-259.
Direct Link  |  

©  2020 Science Alert. All Rights Reserved