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Research Article
 

Effect of Cannabis sativa on Hematological Indices in Rats and Men



Amna H. Mukhtar and Nabiela M. Elbagir
 
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ABSTRACT

This study was designed to assess the effects of injection of different doses of petroleum ether extract of Cannabis sativa on important hematological indices in rats. Similar effects in adult men who had been smoking C. sativa for different periods were also assessed. Blood hemoglobin concentration was not affected in both addicted humans and treated rats, whereas blood erythrocyte count (RBC) and total leukocytes count (TWBC) revealed significant (p<0.05) decreases in the group of rats treated with 0.2 mg/g of the extract and 0.6 mg/g respectively but no changes in addict men groups were observed. Neutrophils and lymphocytes percentages decreased and eosinophils increased significantly (p<0.05) in all treated groups of rats compared to the control group. In the addicted men eosinophils and monocytes showed significantly (p<0.05) lower levels compared to the control group, but lymphocytes percentages were elevated in all addicted men compared to the control group with a significant (p<0.05) difference in the heavy addicted group. These findings showed that injection of C. sativa petroleum extract to the rats resulted in different effects in some parameters compared to those in addicted men smoked C. sativa for long periods. This was clear for the lymphocytes percentage which decreased significantly in the treated rats and showed significantly higher percentage in the addicted men when compared to their controls.

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  How to cite this article:

Amna H. Mukhtar and Nabiela M. Elbagir, 2011. Effect of Cannabis sativa on Hematological Indices in Rats and Men. Pakistan Journal of Nutrition, 10: 313-316.

DOI: 10.3923/pjn.2011.313.316

URL: https://scialert.net/abstract/?doi=pjn.2011.313.316

REFERENCES

1:  Archer, R.K., 1963. The Eosinophil Leukocyte. Blackwell, Oxford, pp: 71-80

2:  Blenkinsopp, E.C. and W.K. Blenkinsopp, 1967. Effect of glucocorticosteroids oneosinophils of rats. J. Endocrinol., 37: 463-469.

3:  Brady, J., R. Curtis and J. Nothstein, 2009. Medical Attributes of Cannabis sativa-Marijuana. Wilkes University, Wilkes-Barre, PA

4:  Cruickshank, E.K., 1976. Physical assessment of 30 chronic cannabis users and 30 matched controls. Ann. N.Y. Acad. Sci., 282: 162-167.
CrossRef  |  

5:  Yahya, M.D. and R.R. Watson, 1987. Immunomodulation by morphine and marijuana. Life Sci., 41: 2503-2510.
CrossRef  |  

6:  Hollister, L.E., H.K. Gillespier, A. Ohlsson, J.E. Lindgren, A. Wahlen and S. Agruell, 1981. Do plasma concentration of δ9THC cannabinol reflect the degree of intoxication. J. Clin. Pharmacol., 21: 171S-177S.

7:  Huber, G.L., V.E. Pochay, W. Pereira, J.W. Shea, W.C. Hinds, M.W. First and G.C. Sornberger, 1980. Marijuana, tetrahydrocannabinol and pulmonary antibacterial defenses. Chest, 77: 403-410.
PubMed  |  

8:  Mahfouz, M., A.B. Makar, M.T. Ghoneim and M.M. Mikhail, 1975. Effect of hashish on brain gamma aminobutyric acid system, blood fibrinolytic activity and glucose and some serum enzymes in the rat. Pharmazie, 30: 772-774.
PubMed  |  Direct Link  |  

9:  Mann, P.E., A.B. Cohen, T.N. Finley and A.J. Ladman, 1971. Alveolar macrophages. Structural and functional differences between nonsmokers and smokers of marijuana and tobacco. Lab. Invest., 25: 111-120.
PubMed  |  

10:  Murikinati, S., E. Juttler, T. Keinert, D.A. Ridder and S. Muhammad et al., 2010. Activation of cannabinoid 2 receptors protects against cerebral ischemia by inhibiting neutrophil recruitment. FASEB J., 24: 788-798.
PubMed  |  

11:  Obonga, W.O., P.O. Osadebe, C.O. Esimone and J. Ihedioha, 2005. Haematotoxic evaluation of crude cannabis resin in rats. J. Pharma. Allied Sci., 3: 278-282.

12:  Perez-Reyes, M., S. Di Guiseppi, K.H. Davis, V.H. Schindler and C.E. Cook, 1982. Comparison of effects of marihuana cigarettes to three different potencies. Clin. Pharmacol. Ther., 13: 617-624.
PubMed  |  

13:  Petersen, H., J. Graham and L. Lemberger, 1976. Marijuana tetrahydrocannabinol and T. cell function. Life Sci., 19: 395-400.

14:  Rosenkrantz, H. and H.J. Esber, 1980. Cannabinoid-induced hormone changes in monkeys and rats. J. Toxicol. Environ. Health, 6: 297-313.
PubMed  |  

15:  Rubin, V. and L. Comitas, 1975. Ganja in Jamica-Mouton and Co., Publishers, the Hague Paris-Salzman, Marijuana and hostility in small group setting. Am. J. Psychiat., 133: 1029-1033.

16:  Steel, R.G.D. and J.H. Torrie, 1960. Principles and Procedures of Statistics. 1st Edn., McGraw Hill, New York, USA., pp: 107-109

17:  Tashkin, D.P., B.J. Shapiro, Y.E. Lee and C.E. Harper, 1976. Subacute effects of heavy marihuana smoking on pulmonary function in healthy men. N. Engl. J. Med., 294: 125-129.
PubMed  |  

18:  UNDCP, 1997. World drug report. Oxford University Press, New York.

19:  Slikker, Jr. W., M.G. Paule, S.F. Ali, A.C. Scallet and J.R. Bailey, 1991. Chronic marijuana smoke exposure in the rhesus monkey: I. Plasma cannabinoid and blood carboxyhemoglobin concentrations and clinical chemistry parameters. Fundamental Applied Toxicol., 17: 321-334.
CrossRef  |  

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